Electrochemical monitoring of biointeraction by graphene-based material modified pencil graphite electrode
Küçük Resim Yok
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Advanced Technology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Recently, the low-cost effective biosensing systems based on advanced nanomaterials have received a key attention for development of novel assays for rapid and sequence-specific nucleic acid detection. The electrochemical biosensor based on reduced graphene oxide (rGO) modified disposable pencil graphite electrodes (PGEs) were developed herein for electrochemical monitoring of DNA, and also for monitoring of biointeraction occurred between anticancer drug, Daunorubicin (DNR), and DNA. First, rGO was synthesized chemically and characterized by using UV-Vis, TGA, FT-IR, Raman Spectroscopy and SEM techniques. Then, the quantity of rGO assembling onto the surface of PGE by passive adsorption was optimized. The electrochemical behavior of rGO PGEs was examined by cyclic voltammetry (CV). rGO-PGEs were then utilized for electrochemical monitoring of surface-confined interaction between DNR and DNA using differential pulse voltammetry (DPV) technique. Additionally, voltammetric results were complemented with electrochemical impedance spectroscopy (EIS) technique. Electrochemical monitoring of DNR and DNA was resulted with satisfying detection limits 0.55 mu M and 2.71 mu g/mL, respectively.
Açıklama
Anahtar Kelimeler
Reduced Graphene Oxide, Pencil Graphite Electrodes, Electrochemical Biosensors, Drug-Dna Interaction, Electrochemical Impedance Spectroscopy, Differential Pulse Voltammetry
Kaynak
Biosensors & Bioelectronics
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
92
