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Öğe Alpha-glutathione-s-transferase can be a biomarker for both drug-related toxicity as well as individual susceptibility(Edizioni Minerva Medica, 2016) Ciba, Melda; Mehmet, A. K.; Karahalil, BensuBACKGROUND: Psychiatric disorders are health concern. The important issue is that the response of treatment of patients is different. Some patients are in remission, some suffer from adverse drug reactions (ADRs). Hepatic function is monitored by liver enzymes. It is important to detect liver injury and its evaluation according to the genetic polymorphisms in early phase of treatment. Alpha-glutathione-s-transfcrase (alpha-CiST) is a sensitive biomarker compared to the liver enzymes. GSTs are phase II conjugation enzymes that detoxify xenobiotic and protect cells from the oxidative stress. GSTMI, GSTT1 and GSTPI are polymorphic. Null genotypes cause lack of activity. Our aim was to investigate whether alpha-GST might be earlier biomarker than liver enzymes for liver injury and impact of individual susceptibility. METHODS: Blood samples before treatment, 10=3 days and 31 months of treatments were taken from 132 psychotic disorder patients in treatment. Serum alpha-GST was measured by Enzyme Linked Immunosorbent Assay (ELISA) and GSTs gene polymorphisms were genotyped, by Pcilymerase Chain Reaction. RESULTS: Our data indicated that alpha-GST might be a btomarket lbr liver injury and GSTs gene polymorphisms had a contribution to the risk of psychotic disorders. CONCLUSIONS: Our results encourage making research on finding more specific biomarker and individual susceptibility with larger sample size.Öğe Metabolomics mapping changed after olanzapine therapy in drug-naive schizophrenia patients-the significant impact of gene polymorphisms(Oxford Univ Press, 2022) Karahalil, Bensu; Elkama, Aylin; Ak, Mehmet; Nemutlu, EmirhanOxidative stress may contribute to the development of schizophrenia and antipsychotics used in schizophrenia treatment may also cause oxidative stress. Gene polymorphisms on antioxidant and repair enzymes are responsible for individual variations and may change the efficacy of olanzapine treatment among schizophrenia patients. In our study, we assessed oxidative stress-related metabolite changes due to genetic polymorphisms on first diagnosed-schizophrenia patients treated with olanzapine. Blood samples (n = 30 patients) were taken before treatment (T1), after 10 +/- 1 days (T2), and after 3 +/- 1 months (T3). T1 served as control for T2 and T3, since it is advantageous to perform on same patient to evaluate the impact of olanzapine only. GSTs (GSTM1, GSTT1, and GSTP1) and OGG1 gene polymorphisms were analyzed by polymerase chain reaction. Changes in metabolites were detected with metabolomics profiling by gas chromatography-mass spectrometry according to each genotype before and after treatment. Multivariate analysis showed that metabolomics profiles differed after olanzapine treatment regardless gene polymorphisms. Tryptophan could be a biomarker in response to olanzapine treatment since its levels were increased after treatment. GSTM1 gene polymorphism caused significant changes in some metabolites after treatment. Urea, palmitic acid, and caprylic acid levels increased and alanine levels decreased in patients with GSTM1 null genotypes after olanzapine. In future, targeted metabolomics with these prominent metabolites and assessing gene expressions of GSTs will be beneficial to understand the mechanism of action.Öğe Serum alpha-GST activity as an alternative biomarker in olanzapine induced hepatotoxicity under the impact of genetic polymorphism of GSTs(Elsevier Ireland Ltd, 2017) Elkama, Aylin; Ak, Mehmet; Nemutlu, Emirhan; Ilik, Nazlican; Karahalil, Bensu[Abstract Not Availabe]
