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Öğe Clinical Evaluation of Acute Pancreatitis Caused by SARS-CoV-2 Virus Infection(Hindawi Ltd, 2021) Vatansev, Hulya; Yildirim, Mehmet Aykut; Kuccukturk, Serkan; Karaselek, Mehmet Ali; Kadiyoran, CengizIntroduction. Coronavirus 2019 disease (COVID-19), caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has spread to more than 200 countries worldwide. We aimed to present acute pancreatitis (AP) cases caused by SARS-CoV-2 viral infection. Methods. The study was conducted retrospectively between April 2020 and June 2020 in Necmettin Erbakan University Meram, Medical Faculty Hospital, and 150 hospitalized patients diagnosed with COVID-19 were included. The degree of acute pancreatitis was determined according to the Atlanta classification. Organ failures of the patients were evaluated in terms of respiratory, cardiovascular, and nephrology according to the modified Marshall scoring (MMS) system, and CTSI (Balthazar score) and Imrie score were determined. Modified Marshall score >= 2 was considered organ failure. Results. A total of 29 patients were diagnosed with acute pancreatitis. All 29 patients with pancreatitis had respiratory failure during hospitalization. After the diagnosis of pancreatitis, there was no change in respiratory failure. According to the Atlanta classification, 19 patients had mild AP and 10 patients had moderate AP. Patients with acute pancreatitis were scored according to the CTSI (Balthazar score), and there were no patients with >= 6 severe pancreatitis. The CTSI score of 4 patients was 3. In addition, the Imrie score of the patients was determined and 8 patients with Imrie score >= 3 were identified. Conclusion. The rate of pancreatic damage in SARS-CoV-2 infection was found to be 19% (n=29) in our study. In our study, we highlight acute pancreatitis as a complication associated with COVID-19 and the importance of pancreatic evaluation in patients with COVID-19 and abdominal pain is demonstrated.Öğe Doğal öldürücü (NK) hücre düşüklüğü olan hastalarda, NK hücre sitotoksitelerinin ve FcγRIIIA gen mutasyonlarının araştırılması(Necmettin Erbakan Üniversitesi Sağlık Bilimleri Enstitüsü, 2020) Karaselek, Mehmet Ali; Kurar, ErcanDoğal öldürücü hücreler (natural killer, NK), organizmayı enfeksiyonlara ve kansere karşı savunmada önemli rol oynayan hücrelerdir. NK hücrelerini organizmada iki önemli görevi bulunmaktadır. Bunlardan birincisi, enfeksiyona neden olan mikroorganizmalara karşı direkt sitotoksik etki, ikincisi ise FcγRIIIA geni aracılığıyla gerçekleşen antikor bağımlı hücresel sitotoksisite (ADCC)'dir. NK hücrelerinin sayı veya fonksiyonunda bozukluk(lar), NK hücre eksikliği/bozukluğu (NKD) olarak adlandırılmaktadır. Bu çalışmada, klinik olarak NKD düşünülen hastalar NK hücre alt grupları, NK hücre sitotoksisitesi ve FcγRIIIA gen mutasyon(lar)u açısından araştırılması ve klinik, laboratuvar ve fonksiyonel analiz sonuçları ile FcγRIIIA gen mutasyon(lar)u arasındaki ilişkinin değerlendirilmesi amaçlandı. Çalışmaya Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi Çocuk Allerji ve İmmünoloji polikliniğine ilk defa başvuran hastalar ile daha önceden takipli 10 hasta ve 7 sağlıklı bireyden oluşan kontrol grubu dahil edildi. Tüm hasta ve kontrol kan örneklerinden, periferik kan lenfosit oranları ve NK hücre alt grupları oranları akım sitometri yöntemiyle, NK hücrelerinin fonksiyonları ise sitotoksisite testi ile değerlendirildikten sonra yeni nesil dizileme yöntemi ile FcγRIIIA gen mutasyonları araştırıldı. Periferik kan akım sitometri yöntemi ile değerlendirilen NK hücre alt grup analizinde CD56brightCD16neg, CD56brightCD16int ve CD56dimCD16hi olmak üzere 3 farklı NK hücre alt grubu belirlendi. Hastaların NK hücre alt grupları kontrol grubu ile karşılaştırıldığında CD56brightCD16neg hücre oranında statistiksel olarak anlamlı bir farklılık tespit edilmedi. CD56brightCD16int ve CD56dimCD16hi hücre oranları kontrol grubu ile karşılaştırıldığında hastalarda anlamlı derecede düşük olduğu tespit edildi. NK hücre sitotoksisitesi, K562 lizisine bağlı yöntem ile değerlendirildi. NK hücre sitotoksisite analizi sonucunda hasta ile kontroller arasında K562 miktarındaki oransal olarak azalmanın istatistiksel olarak anlamlı olduğu tespit edildi (p<0,001). Hastalardaki K562 hücre azalma oranı kontrollere kıyasla istatistiksel olarak daha az azaldığı tespit edildi. Yeni nesil dizileme yöntemi ile FcγRIIIA geninin tüm exom analizinde, ekzon 4 (rs396991) ve ekzon 3 (rs10127939)'de polimorfizmler olduğu belirlendi. Ayrıca dizileme sonucunda FcγRIIIA ile FcγRIIIB ile benzerlik gösteren olası kimerik bir bölge (ekzon 1 ile ekzon 2 ile ekzon 1 arasındaki intron bölgesi) tespit edildi. Sonuç olarak bu tez çalışmasında FNKD düşünülen hastalar ileri fonksiyonel ve genetik analizler ile değerlendirildi. Hastalarda NK sayı ve fonksiyonları yönünden farklılıklar tespit edildi. FcγRIIIA geni ile ilgili tespit edilen polimorfizmler Türkiye popülasyonunda yapılmış ilk değerlendirme niteliğinde olup, NK hücre araştırmaları alanında yapılacak yeni çalışmalara ışık tutması beklenmektedir.Öğe Effect of intensive training on immune system cells in elite female weightlifters(Assoc Medica Brasileira, 2024) Karaselek, Mehmet Ali; Kuccukturk, Serkan; Duran, TugceOBJECTIVE: This study aimed to investigate the effects of intense weightlifting training on lymphocyte and natural killer cell subgroups, which are the major cells of the immune system, in elite female weightlifters.METHODS: A total of 20 elite female weightlifters were evaluated using flow cytometry before training (pre-T), immediately after training (post-T), and after a 120-min rest period (rest-T).RESULTS: Post-T and rest-T showed significant decreases in helper T (Th) and cytotoxic T compared with pre-T (p=0.045, p<0.001 and p=0.05, p<0.001, respectively). B and natural killer cells were higher in post-T and rest-T than in pre-T. The increase in B cells was significant in pre-T/rest-T (p<0.001) but not in pre-T/post-T (p=0.122). Intense training significantly increased natural killer cells in both post-T and rest-T (p<0.001). CD56brightand CD56dim natural killer cell subgroups were significantly lower in post-T and rest-T than in pre-T (p=0.005, p=0.006 and p<0.001, p=0.004, respectively).CONCLUSION: This study shows that intense weightlifting alters peripheral lymphocyte and natural killer subgroup ratios, being the first investigation in this field.Öğe Effect of intensive training on immune system cells in elite female weightlifters(Assoc Medica Brasileira, 2024) Karaselek, Mehmet Ali; Kuccukturk, Serkan; Duran, TugceOBJECTIVE: This study aimed to investigate the effects of intense weightlifting training on lymphocyte and natural killer cell subgroups, which are the major cells of the immune system, in elite female weightlifters.METHODS: A total of 20 elite female weightlifters were evaluated using flow cytometry before training (pre-T), immediately after training (post-T), and after a 120-min rest period (rest-T).RESULTS: Post-T and rest-T showed significant decreases in helper T (Th) and cytotoxic T compared with pre-T (p=0.045, p<0.001 and p=0.05, p<0.001, respectively). B and natural killer cells were higher in post-T and rest-T than in pre-T. The increase in B cells was significant in pre-T/rest-T (p<0.001) but not in pre-T/post-T (p=0.122). Intense training significantly increased natural killer cells in both post-T and rest-T (p<0.001). CD56brightand CD56dim natural killer cell subgroups were significantly lower in post-T and rest-T than in pre-T (p=0.005, p=0.006 and p<0.001, p=0.004, respectively).CONCLUSION: This study shows that intense weightlifting alters peripheral lymphocyte and natural killer subgroup ratios, being the first investigation in this field.Öğe Effective anticancer agents based-on two Pillar[5]arene derivatives for pancreas cancer cell lines: synthesis, apoptotic effect, caspase pathway(Springer, 2023) Karaselek, Mehmet Ali; Kuccukturk, Serkan; Duran, Tugce; Kursunlu, Ahmed Nuri; Ozmen, Mustafa; Bozdag, Ceren; Alkan, SelmanThis study aimed to evaluate the possible anticancer effects of two different pillar[5]arene derivatives (5Q-[P5] and 10Q-P[5]) on two different pancreatic cancer cell lines in vitro. For this purpose, changes in the expression of major genes that play a role in apoptosis and caspase pathways were investigated. Panc-1 and BxPC-3 cell lines were used in the study and the cytotoxic dose of pillar[5]arenes was determined by the MTT method. Changes in gene expression after pillar[5]arenes treatment were evaluated by real-time polymerase chain reaction (qPCR). Apoptosis was studied by flow cytometry. As a result of analysis, it was determined that proapoptotic genes and genes involved in major caspase activation were upregulated and antiapoptotic genes were down-regulated in Panc-1 cell line treated with pillar[5]arenes. Flow cytometric apoptosis analysis also showed an increased apoptosis rate in this cell line. On the contrary, although MTT analysis showed cytotoxic effect in BxPC-3 cell line treated with two pillar[5]arene derivatives, the apoptosis pathway was not active. This suggested that it may activate different death pathways for BxPC-3 cell line. Thus, it was first determined that the pillar[5]arene derivatives reduced cancer cell proliferation on pancreatic cancer cells.Öğe Evaluation of coagulation with TEG in patients diagnosed COVID-19(Tubitak Scientific & Technological Research Council Turkey, 2022) Vatansev, Hulya; Karaselek, Mehmet Ali; Yilmaz, Resul; Kuccukturk, Serkan; Topal, Ahmet; Yosunkaya, Sebnem; Kucuk, AdemBackground and aim: A high D-dimer level may indicate the risk of coagulopathy and mortality in COVID-19 patients. Thromboelastography (TEG) is a test that evaluates clot formation and fibrinolysis in real-time, unlike routine coagulation tests. The study aimed to investigate the coagulation process with TEG in patients diagnosed with COVID-19. Materials and Methods: The study was performed at our university hospital, chest diseases outpatient clinic as a cross-section study. A total of 51 patients with 23 high D-dimer levels group (HDG) and 28 low D-dimers group (LDG) were included in the study. TEG analysis was performed at the pretreatment evaluation in these two groups. Results: D-dimer and fibrinogen levels of the HDG were higher than those of the LDG (550 vs. 90 ng/mL, p < 0.001; 521 vs. 269 mg/ dL, p < 0.001, respectively). In TEG analysis, HDG's R and K values were lower than LDG, and HDG's Angle, MA, and CI values were higher than LDG (p = 0.037; p < 0.001; p < 0.001; p < 0.001; p < 0.001, respectively). ROC curve analysis suggested that the optimum TEG parameters cut-off points for thrombosis risk were as below: for K was <_2.1 min, for R was <_6.1 min, for Angle was >62 degrees, MA was 60.4 mm. Conclusion: Our study showed that the risk of thrombosis might increase in COVID-19 patients who are not hospitalized in the intensive care unit. Thrombosis risk should be investigated with TEG analysis and laboratory tests in every patient diagnosed with COVID-19, and treatment should be started for risky patients.Öğe Evaluation of transcription factors and cytokine expressions of T-cell subsets in CD19 deficiency and their possible relationship with autoimmune disease(Wiley, 2024) Kueccuektuerk, Serkan; Karaselek, Mehmet Ali; Duran, Tugce; Reisli, IsmailCD19 deficiency is a rare, predominantly antibody deficiency, and there are few studies showing that it can be seen in autoimmune diseases. The aim of study was evaluated to transcription factor and cytokine expressions of helper T (Th)-cell subsets in CD19 deficiency and the possible mechanism role of this factor expression in autoimmune disease. Transcription factor and cytokine expressions of Th1, Th2, Th17, and regulatory T (Treg) cells were investigated by real-time polymerase chain reaction (qPCR) method. In the study, in the patient/control comparison, transcription factor and cytokine expressions of Th1 (T-bet, STAT1, and STAT4) were found to be significantly downregulated, but IFN-gamma was significantly upregulated in patients. Th2 factor GATA3, STAT6, IL-4, and IL-5 were significantly downregulated. For Th17, ROR gamma t was downregulated while IL-22 was upregulated. In the heterozygous/control comparison, there was no significant change in gene expressions other than IL-5. T-bet, STAT1, GATA3, IL-4, ROR gamma t, FoxP3, and TGF-beta were significantly downregulated in the patient/heterozygous comparison. It was revealed for the first time that the expression of the transcription factors and cytokines in CD19 deficiency. These findings might be showing the predominance of Th1 factors and suppressed Treg factors which could be related with autoimmunity in CD19 deficiency.Öğe Impacts of potential anticancer agents based on pillar[5]arene for head and neck squamous cell carcinoma cells(Springer, 2023) Kuccukturk, Serkan; Karaselek, Mehmet Ali; Duran, Tugce; Kursunlu, Ahmed Nuri; Ozmen, MustafaPurposeThis study was conducted to investigate impacts of potential anticancer (associated with apoptosis and caspase pathways) of two newly synthesized derivatives of pillar[5]arene, named as d-Q-P5 and p-Q-P5, on Squamous cell carcinomas of the head and neck (HNSCC) cells.Materials and methodsThe MTT method was used to determine the IC50 doses of the derivatives on HNSCC cells, and the changes in gene expression were analyzed by real-time polymerase chain reaction (qPCR). The apoptosis change was confirmed by flow cytometry analysis.ResultsThe results showed that the d-Q-P5 and p-Q-P5 effectively inhibited the proliferation of the cells by upregulating proapoptotic genes (Bax, Bad, p53, Bak, and Apaf-1) and genes involved in the caspase pathway (Casp2, Casp3, and Casp9), while downregulating the antiapoptotic gene (Bcl-2).ConclusionsThis study is the first to demonstrate the potential anticancer effects of these two agents on HNSCC cells by positively regulating apoptosis gene expression.Öğe THE INFILTRATION OF T AND B LYMPHOCYTES AND NK CELLS IN KIDNEY BIOPSIES OF PATIENTS WITH FABRY DISESE UNDER ENZYME REPLACEMENT THERAPHY(Oxford Univ Press, 2020) Turkmen, Kultigin; Esen, Hasan; Karaselek, Mehmet Ali; Celik, Seyma Celikbilek; Baloglu, Ismail; Guner, Sukru; Reisli, Ismail[Abstract Not Availabe]Öğe Intrauterine detection of DCLRE1C (Artemis) mutation by restriction fragment length polymorphism(Wiley, 2019) Karaselek, Mehmet Ali; Kapakli, Hasan; Keles, Sevgi; Guner, Sukru Nail; Celik, Seyma Celikbilek; Kurar, Ercan; Reisli, Ismail[Abstract Not Availabe]Öğe Outcome of Thrombotic Thrombocytopenic Purpura Patients: A Single-Center Experience(Galenos Yayincilik, 2019) Ceneli, Ozcan; Yilmaz, Seda; Karaselek, Mehmet Ali; Camli, Kazim[Abstract Not Availabe]Öğe Retrospective analysis of patients with severe combined immunodeficiency and alternative diagnostic criteria: A 20-year single centre experience(Wiley, 2023) Korkmaz, Sevim Busra; Karaselek, Mehmet Ali; Aytekin, Selma Erol; Tokgoz, Huseyin; Reisli, Ismail; Guner, Sukru; Keles, SevgiSevere combined immunodeficiency (SCID) is an inborn errors of immunity (IEI) disorder characterized by impairment in the development and function of lymphocytes and could be fatal if not treated with hematopoietic stem cell transplant in the first 2 years of life. There are various diagnostic criteria for SCID among different primary immunodeficiency societies. We retrospectively evaluated clinical and laboratory findings of 59 patients followed up with the diagnosis of SCID at our clinic over the past 20 years in order to develop an algorithm that would help diagnosis of SCID for the countries where a high ratio of consanguineous marriage is present because these countries have not launched TREC assay in their newborn screening programs. The mean age at diagnosis was 5.80 +/- 4.90 months, and the delay was 3.29 +/- 3.99 months. The most common complaint and physical examination findings were cough (29.05%), eczematous rash (63%) and organomegaly (61%). ADA (17%), Artemis (14%), RAG1/2 (15%), MHC Class II (12%) and IL-2R (12%) deficiencies were the most common genetic defects. Lymphopenia (87.5%) was the most frequent abnormal laboratory finding and below 3000/mm(3) in 95% of the patients. The CD3(+) T cell count was 300/mm(3) and below in 83% of the patients. As a result, a combination of low lymphocyte count and CD3 lymphopenia for SCID diagnosis would be more reliable for countries with high rate of consanguineous marriage. Physicians should consider diagnosis of SCID in a patient presenting with severe infections and lymphocyte counts below 3000/mm(3) under 2 years of age.Öğe Successful management of refractory immune thrombocytopenia associated with massive alveolar hemorrhage(Pergamon-Elsevier Science Ltd, 2015) Bilgin, Aynur Ugur; Karaselek, Mehmet Ali; Bilgin, RasimImmune thrombocytopenia (ITP) is an acquired immune-mediated disease characterized by persistant thrombocytopenia. Onset of the disease and the clinical course is highly variable, but the disease typically has a benign course. Patients rarely develop serious life-threatening bleeding. Herein, a case with refractory ITP and massive alveolar hemorrhage who was successfully treated with a fast and sequential approach is presented for the first time. (C) 2015 Elsevier Ltd. All rights reserved.Öğe Successful management of thrombotic thrombocytopenic purpura associated with pregnancy(Pergamon-Elsevier Science Ltd, 2014) Bilgin, Aynur Ugur; Karaselek, Mehmet Ali; Camli, KazimThrombotic thrombocytopenic purpura (TTP) is an uncommon, severe, potentially life-threatening disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, altered mental status, fever, and renal abnormalities. It can be seen at any age or sex but affects women of childbearing age more commonly. Pregnancy is known as one of the most common precipitating events for the onset of TTP and occurs mostly in the late third trimester or during the puerperium. Because of relatively low prevalence of pregnancy-related TTP, here we report the clinical characteristics and successful outcomes of 7 women with pregnancy-related TTP. Median age of patients was 25 (19-32). While 4 out of 7 women were primiparous, others were multiparous. Total plasma exchange (TPE) procedure was started within 24 h after admission to our hospital. All patients got into complete remission without any maternal mortality. Fetal mortality was found to be 28%. Pregnancy-related TTP is still associated with high maternal and fetal mortality rates. However, the prognosis of TTP has improved dramatically with early diagnosis and plasma-based therapies. (C) 2014 Elsevier Ltd. All rights reserved.Öğe Synthesis and evaluation of anticancer effect of a novel molecule based-on pillar[5]arene including multi quinoline units(Springer Birkhauser, 2020) Gunes, Canan Eroglu; Karaselek, Mehmet Ali; Kursunlu, Ahmed Nuri; Ozmen, Mustafa; Kurar, ErcanIn this study, pillar[5]arene containing ten terminal alkynyl-functional group was prepared and carried out an optimization procedure for target molecule with using 5-hydroxyquinoline molecule known with biological activity. The novel molecule was named as iso-QP[5] and characterized by FT-IR, melting point, NMR, elemental analysis, and mass spectroscopy. The aim was to evaluate the anticancer effects of iso-QP[5] on MCF7 human breast cancer cells. The IC50 dose of iso-QP[5] in MCF7 cells was found to be 25 mu M for 24 h using XTT assay. Expressions of eight genes for apoptosis were determined by qPCR. The results showed a significant increase in the expression of BAX, CASP3, CASP9, CYCS, and P53 genes after treatment with iso-QP[5] in MCF7 cells. However, iso-QP[5] treatment significantly decreased expression of BCL2 and CASP8 genes. It is concluded that novel molecule iso-QP[5] caused apoptosis by regulating genes that are important in apoptosis in human breast cancer cells.Öğe T Hücrelerin Eş Uyaran ve İnhibitörleri: CD28 Ailesi(2016) Karaselek, Mehmet Ali; Reisli, İsmailHücresel bağışıklıkta rol oynayan T lenfositlerin, immün yanıt oluşturabilmesi için aktive olmaları gerekmektedir. T lenfosit aktivasyonu için iki sinyal gereklidir. Bu sinyallerden birincisi antijen tarafından sağlanır. İkinci sinyal ise, eş uyaran moleküller tarafından sağlanmaktadır. Yıllar önce T hücrelerin aktivasyonu için herhangi bir eş uyarana ihtiyaç duymadığına inanılmaktaydı. Ancak yapılan in vitro ve in vivo çalışmalar T hücrelerin aktivasyonu ve büyümesi için eş uyaranlara ihtiyaç duyduklarını göstermişlerdir. T hücre aktivasyonu ve büyümesi gerekli eş uyarılar, CD28 ailesi ve tümör nekrosis faktör (TNF) ailesi üyelerine ait olan moleküller tarafından sağlanır. CD28 ailesine ait 4 adet eş uyaran molekül tanımlanmıştır. Bunlar CD28, indüklenebilir T hücre kostimulatör sistemi (ICOS), sitotoksik T lenfosit ilişkili protein 4 (CTLA-4, CD152) ve programlanmış hücre ölümü proteini 1 (PD-1) molekülleridir. CD28 ve ICOS T hücre aktivasyonunda pozitif etkili iken, CTLA-4 ve PD-1 molekülleri negatif etki göstermektedir. Bu derlemede CD28 ailesine ait eş uyaran moleküller, bu moleküllere ait ligandlar ve eş uyaran moleküllerin ligandlarla etkileşimleri tartışılmaktadır.
