Impacts of potential anticancer agents based on pillar[5]arene for head and neck squamous cell carcinoma cells
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
PurposeThis study was conducted to investigate impacts of potential anticancer (associated with apoptosis and caspase pathways) of two newly synthesized derivatives of pillar[5]arene, named as d-Q-P5 and p-Q-P5, on Squamous cell carcinomas of the head and neck (HNSCC) cells.Materials and methodsThe MTT method was used to determine the IC50 doses of the derivatives on HNSCC cells, and the changes in gene expression were analyzed by real-time polymerase chain reaction (qPCR). The apoptosis change was confirmed by flow cytometry analysis.ResultsThe results showed that the d-Q-P5 and p-Q-P5 effectively inhibited the proliferation of the cells by upregulating proapoptotic genes (Bax, Bad, p53, Bak, and Apaf-1) and genes involved in the caspase pathway (Casp2, Casp3, and Casp9), while downregulating the antiapoptotic gene (Bcl-2).ConclusionsThis study is the first to demonstrate the potential anticancer effects of these two agents on HNSCC cells by positively regulating apoptosis gene expression.
Açıklama
Anahtar Kelimeler
Head And Neck Squamous Cell Carcinoma, Pillar[5]Arene, Apoptosis, Caspase
Kaynak
Journal Of Cancer Research And Clinical Oncology
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
149
Sayı
11
