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Öğe Ameliorating the effects of Adalimumab on rabbits with experimental cerebral vasospasm after subarachnoid hemorrhage(Turkish Assoc Trauma Emergency Surgery, 2020) Toguslu, Gokhan; Erdi, Mehmet Fatih; Arac, Densel; Keskin, Fatih; Kilinc, Ibrahim; Cuce, GokhanBACKGROUND: Adalimumab (ADA), which is a new-generation recombinant human monoclonal antibody for tumor necrosis factor a (TNF alpha), has strong anti-inflammatory effects. The role of enhanced inflammation is well established for the development and progression of cerebral vasospasm. Investigated in the present study is the probable ameliorating and neuroprotective effects of ADA in rabbits using a cerebral vasospasm model with biochemical and histopathological methods. METHODS: Thirty male New-Zealand white rabbits were randomly divided into control, subarachnoid hemorrhage (SAH) only and SAH plus ADA treatment groups. SAH was established as a single cisterna magna autologous arterial blood injection. ADA treatment was started just after intracisternal blood injection and continued for 72 hours once a day. The animals were sacrificed 72 hours after the induction of SAH, serum and brainstem tissue obtained for investigations. RESULTS: Brainstem tissue and plasma levels of tumor necrosis factor-alpha and Interleukin-1 beta, brainstem tissue Matrix metalloproteinase-9 levels increased after SAH and partly decreased after treatment. Plasma levels of brain-derived neurotrophic factor decreased after SAH and partly restored after treatment. ADA treatment significantly increased the mean cross-sectional area of the vasospastic basilar arteries, reduced the basilar artery wall thickness and also ameliorates enhanced endothelial apoptosis. CONCLUSION: Findings obtained in this study suggest that ADA is an effective neuroprotective agent for ameliorating cerebral vasospasm in experimental rabbit vasospasm.Öğe Ameliorating the effects of Adalimumab on rabbits with experimental cerebral vasospasm after subarachnoid hemorrhage(Turkish Assoc Trauma Emergency Surgery, 2020) Toguslu, Gokhan; Erdi, Mehmet Fatih; Arac, Densel; Keskin, Fatih; Kilinc, Ibrahim; Cuce, GokhanBACKGROUND: Adalimumab (ADA), which is a new-generation recombinant human monoclonal antibody for tumor necrosis factor a (TNF alpha), has strong anti-inflammatory effects. The role of enhanced inflammation is well established for the development and progression of cerebral vasospasm. Investigated in the present study is the probable ameliorating and neuroprotective effects of ADA in rabbits using a cerebral vasospasm model with biochemical and histopathological methods. METHODS: Thirty male New-Zealand white rabbits were randomly divided into control, subarachnoid hemorrhage (SAH) only and SAH plus ADA treatment groups. SAH was established as a single cisterna magna autologous arterial blood injection. ADA treatment was started just after intracisternal blood injection and continued for 72 hours once a day. The animals were sacrificed 72 hours after the induction of SAH, serum and brainstem tissue obtained for investigations. RESULTS: Brainstem tissue and plasma levels of tumor necrosis factor-alpha and Interleukin-1 beta, brainstem tissue Matrix metalloproteinase-9 levels increased after SAH and partly decreased after treatment. Plasma levels of brain-derived neurotrophic factor decreased after SAH and partly restored after treatment. ADA treatment significantly increased the mean cross-sectional area of the vasospastic basilar arteries, reduced the basilar artery wall thickness and also ameliorates enhanced endothelial apoptosis. CONCLUSION: Findings obtained in this study suggest that ADA is an effective neuroprotective agent for ameliorating cerebral vasospasm in experimental rabbit vasospasm.Öğe A Case of Upper Thoracic Intradural-Extramedullary Multiple Meningiomas(Lippincott Williams & Wilkins, 2013) Keskin, Fatih; Kalkan, Erdal; Karatas, YasarIntradural spinal tumors are classified into extramedullary and intramedullary tumors. The most frequent intradural-extramedullary tumors are meningiomas and neurinomas. Meningiomas mostly occur in thoracic region and are generally located at the dorsal aspect. Multiplicity is a rare condition for spinal meningiomas, and multiple spinal meningiomas are usually associated with neurofibromatosis. In this report, we present a case of thoracic spinal multiple meningiomas which was located at the ventral aspect and not associated with neurofibromatosis.Öğe Clinical evaluation of 34 cases treated with sequestrectomy: Minimum two year follow up(Elsevier Sci Ltd, 2017) Aydin, Murat; Sasani, Mehdi; Oktenoglu, Tunc; Durmaz, Mehmet Ozan; Bozkus, Hakan; Keskin, Fatih; Ozer, Ali Fahir[Abstract Not Availabe]Öğe Clinical Results of Anterior Odontoid Screw Fixation for Type II Odontoid Fractures(Turkish Neurosurgical Soc, 2014) Keskin, Fatih; Gomleksiz, Cengiz; Sasani, Mehdi; Oktenoglu, Tunc; Suzer, Tuncer; Ozer, Ali FahirAIM: In this study, 31 patients with a diagnosis of Type II odontoid fractures were reported. All patients were treated with anterior transodontoid screw fixation and clinical outcomes were reported. MATERIAL and METHODS: In this study, the retrospective clinical analysis of 31 patients with traumatic type II odontoid fractures who were treated through anterior transodontoid fixation in Neurosurgery Department at VKV American Hospital between 1998 and 2012 was performed. The age, sex, cause of injury, diagnosis time, neurological examination before and after surgery, follow-up period were evaluated. The neurological status of patients was classified according to the Frankel scale. RESULTS: In 4 patients, 2 transodontoid screws were inserted. The mean hospital stay was 3.35 days. Posterior occipito-cervical fusion was done in 1 patient due to the lack of fusion in the first operation. No vascular injury, screw malposition, infection, neurologic deterioration, or complications were observed during the peroperative and postoperative stage. The mean postoperative follow-up period was 36 months after surgery. Radiological imaging of patients were performed at the early and late postoperative stage. CONCLUSION: We found satisfying fusion rates and better patient comfort during the postoperative period. We think that stabilization and fusion through a transodontoid screw is a minimal invasive method.Öğe Coccygectomy for coccygodynia: A single-center experience(Lippincott Williams & Wilkins, 2023) Izci, Emir Kaan; Keskin, FatihSpine fractures are most commonly observed among older people due to weak bones and irregular postures. These fractures of 10 lead to coccygodynia. Women are 5 times more prone to developing coccygodynia as compared to men. Initially, different painkillers, physiotherapy, and other non-surgical treatments are recommended to reduce the pain. However, in case of the failure of these treatments, a surgical procedure (named coccygectomy) is considered to remove the tailbone to achieve effective outcomes. The main aim of this study is to determine the effectiveness of coccygectomy in treating coccygodynia (after the failure of different non-surgical treatments) in the context of patients who were treated at Konya City Hospital neurosurgery department, Turkey. In this study, a total of 14 cases of coccygodynia treated with coccygectomy at Konya City Hospital were selected from the period of August 2020 and January 2022. These patients were given different treatments for 6 to 7 months before the operation. Therefore, due to the lack of positive outcomes, a coccygectomy was performed by a senior surgeon. Visual analogue scale was used to assess the pain of the patients after the surgery. Statistical Package for the Social Sciences was used for statistical analysis. The level of significance was set at P < .05. The results of this study showed that more women suffered from coccygodynia as compared to men. Most of the patients had Type I (35.7%) and Type IV (35.7%) coccyx. The visual analogue scale was reduced after the surgery (P < .0011), showing positive outcomes. Ten (71.4%) cases showed excellent outcomes, whereas only one (7.1%) case showed poor outcomes after the surgery, and wound infections were observed in two (14.2%) cases post-operatively. The results obtained from this study concluded that in case of failure of conservative non-surgical treatments for coccygodynia, coccygectomy has emerged as an effective surgical method which is recommended by various surgeons to reduce the pain by removing the tailbone. Additionally, only a few complications such as infection as a relevant concern after this type of surgery were observed, and most of the patients were satisfied with the outcomes and also recommended it to others.Öğe Congenital Carotid-Jugular Fistula in a Child A Case Report(Sage Publications Inc, 2014) Erdi, Fatih; Kaya, Bulent; Keskin, Fatih; Koc, Osman; Karatas, Yasar; Kalkan, ErdalCongenital carotid-jugular (CJ) fistula of the neck is a very rare clinical entity that has various causes. The CJ fistulas are particularly prone to complications unlike other peripheral arteriovenous fistulas. The aim of this report is to present a case of a CJ fistula between the external carotid and the external jugular vein, which was successfully closed with detachable balloon by an endovascular approach. A 14-year-old child was admitted to our clinic with a pulsatile neck swelling. There was no previous history of trauma. A high-flow fistula between the external carotid and the external jugular vein was determined. The fistula was closed with detachable balloon by an endovascular approach. The postoperative angiogram demonstrated complete resolution of the fistula. Endovascular treatment of CJ fistulas with detachable balloons is a safe and less traumatic technique and may be an effective alternative to the open surgery in selected patients.Öğe Deneysel epidural fibroziste adezyon bariyerleri ile chıtın'in etkisinin karşılaştırılması(Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi, 2008) Keskin, Fatih; Baysefer, AlperTavşanlarda oluşturulan deneysel epidural fibrozis sonrası adezyon bariyerleri ile chitin'in etkisini karşılaştırmak için yapılan deneysel çalışmada 4 farklı grupta toplam 28 adet tavşan kullanıldı. Deneklere ketamin ve ksilazin anestezisi altında L5 total laminektomi uygulandı. I. grup kontrol grubu olup laminektomi sahasına herhangi bir madde konulmadı. II. grup laminektomi sahasına duragen plus, III. grup laminektomi sahasına Adcon-L, IV. grup laminektomi sahasına chitin koyulan grup olarak belirlendi. Bütün denekler 6 hafta sonra sakrifiye edildi. Alınan kesitler ışık mikroskobu altında histopatolojik olarak incelendi ve gruplar arası karşılaştırma yapıldı. Çalışmamızda kontrol grubunda ortalama grade 3 fibrozis bulundu ve diğer gruplar arasında istatiksel olarak anlamlı fark tespit ettik (p<0,05). Grup 2,3,4 kendi arasında epidural fibrozisi önlemek açısından istatisksel olarak anlamlı fark tespit edilmedi.Sonuç olarak kullanılan tüm maddeler postoperatif epidural fibrozisi azaltmaktadır. Yeni bir ürün olan chitin'in adezyon bariyerleri arasında bir seçenek olabileceği kanaatine varıldı. Ancak bu sonuçların kliniğe uygulanabilirliği açısından daha ayrıntılı ve ileri çalışmalara ihtiyaç vardır.Öğe Distal posterior inferior serebellar arter anevrizması: Olgu sunumu(2013) Keskin, Fatih; Kaya, Bülent; Üstün, Mehmet Erkan; Uygun, Mehmet AliKırk bir yaşında bayan hasta spontan subaraknoid hemoraji tanısı ile servisimize yatırıldı. Bilgisayarlı beyin tomografisinde bazal sisternlerde subaraknoid kanama ile uyumlu hiperdens alanlar görüldü. Çekilen serebral anjiografide sol PICA'da distal yerleşimli sakküler anevrizma tespit edildi. Hastaya median vertikal insizyon ile sol suboksipital kraniyektomi uygulandı. Distal yerleşimli PICA anevrizması görülerek klipe edildi. Distal PICA anevrizmaları nadir görülen anevrizmalardır ve distal PICA anevrizmalarında en iyi tedavi yöntemi anevrizmanın klipe edilerek dolaşımdan uzaklaştırılmasıdır.Öğe The effects of steroids in traumatic thoracolumbar junction patients on neurological outcome(Turkish Assoc Trauma Emergency Surgery, 2019) Ilik, Mustafa Kemal; Keskin, Fatih; Erdi, Fatih; Kaya, Bulent; Karatas, Yasar; Kalkan, ErdalBACKGROUND: In this study, we aim to evaluate the potential effects of methylprednisolone on the neurological outcome of spinal cord injury (SCI) patients with thoracolumbar junction (T10-L1) spine fractures. METHODS: The data from 182 SCI patients who sustained a thoracolumbar junction spine fracture were operated by us between September 2008 to January 2015 were analysed retrospectively. The patients were divided into two groups: Group I underwent methylprednisolone treatment in conjunction with early surgical intervention, while group 2 underwent only early surgical intervention without methylprednisolone treatment. American Spinal Injury Association (ASIA) motor index scores of the patients were evaluated and compared with statistical methods at admission and at the first-year follow-up. RESULTS:The main follow-up period was 14.4 +/- 1.4 months in group 1 and 13.6 +/- 1.7 months in group 2. Initial and last follow-up ASIA scores of the patients were similar between groups (p>0.05), but the complication rate was significantly high in group 1 (p<0.05). CONCULSION: The findings showed that steroids have no significant beneficial effects on the neurological outcome but have significant side effects and leads to increased complication rate in SCI patients.Öğe Endovascular management of a collateral network aneurysm in a patient with spontaneous internal carotid artery occlusion(Sage Publications Inc, 2015) Kaya, Bulent; Erdi, Fatih; Keskin, Fatih; Kalkan, Erdal; Koc, OsmanSpontaneous non-moyamoya'' arterial occlusion of the intracranial arteries is very unusual. Progressive occlusion of a major intracranial artery, independently from the etiology, can lead to the development of collateral arterial networks that supply blood flow to distal territories beyond the occlusion. These collateral arteries are typically small and conduct low flows, but the hemodynamic stress within them can lead to aneurysm formation within the collateral network. In this report we present a case of spontaneous internal carotid artery occlusion and collateral network aneurysm for the first time in the literature and discuss the main features of the etiology and endovascular treatment of this rare, challenging aneurysm.Öğe Endovascular treatment of complex intracranial aneurysms by pipeline flow-diverter embolization device: a single-center experience(Maney Publishing, 2015) Keskin, Fatih; Erdi, Fatih; Kaya, Bulent; Poyraz, Necdet; Keskin, Suat; Kalkan, Erdal; Ozbek, OrhanObjective: Endovascular coil embolization has become an effective treatment modality for most intracranial aneurysms. However, complex aneurysms including large and giant aneurysms, fusiform shaped aneurysms, wide necked aneurysm, or small aneurysm that are unsuitable for coil embolization are still deterrent to be treated. Flow diversion is a novel concept that is applied in the treatment of these complex intracranial aneurysms. Method: We review the results and important features of 25 aneurysms in 24 patients who underwent endovascular treatment by using the pipeline flow-diverter embolization device. Result: At 6 month follow-up, all aneurysms (100%) showed total occlusion in our series. Only one patient who had giant vertebrobasilar aneurysm experienced major complication related to endovascular treatment. Discussion: We suggest that parent artery reconstruction via flow diversion with the PED is a valid and safe treatment modality.Öğe Endovascular treatment of intracranial aneurysms using the Woven EndoBridge (WEB) device: retrospective analysis of a single center experience(Elsevier Science Inc, 2020) Kaya, Hasan E.; Bakdik, Suleyman; Keskin, Fatih; Erdi, Mehmet F.; Koc, OsmanIntroduction: Woven EndoBridge (WEB) is an innovative device for the treatment of intracranial aneurysms especially wide-necked bifurcation aneurysms. Here we present our experience with the WEB device. Material and methods: Patients treated using only the WEB device between September 2014 and November 2018 were included in the study. Follow up imaging studies and medical records of the patients were retrospectively reviewed. Results: Forty-two aneurysm of 42 patients (27 female, 15 male; median age: 56, range: 32-76) were treated using the WEB device. The mean diameter of the aneurysms was 6.6 mm (range: 3-12 mm). The neck diameter was >= 4 mm in 57% of the aneurysms. The locations of the aneurysms were the middle cerebral artery bifurcation in 29 (69%), basilar tip in 5 (12%), anterior communicating artery in 5 (12%), internal carotid artery tip in 2 (5%), and M1 segment of the middle cerebral artery in 1 (2%) of the patients. Five patients had subarachnoid hemorrhage due to aneurysm rupture. The device could be successfully deployed in all of the cases. There were control imaging studies available for 36 patients who were followed up for a median of 7 months (range: 1-33 months). Adequate occlusion was observed in 35 of these 36 patients (97%). There was no treatment related morbidity or mortality. Conclusion: Although long term follow-up data are not available, WEB intrasaccular flow disruptor seems to be effective and safe for intracranial bifurcation aneurysm treatment in the mid-term follow up.Öğe Evaluation of accuracy, reliability, quality, and readability of online patient information materials on coccyx injury(Lippincott Williams & Wilkins, 2023) İzci, Emir Kaan; Keskin, FatihThe aim of this research is to evaluate the websites containing “coccydynia, coccyx trauma or fracture” in terms of readability, reliability, accuracy, and quality. Searches for “coccydynia, coccyx trauma, coccyx fracture” were carried out in the 3 most used search engines in the USA: Google, Yahoo, and Bing in February 2022. A total of 141 websites were rated by 2 different neurosurgeons for the “Global Quality Score” and “Alexa Popularity Rank.” 97.2% of the sites examined include the definition of the disease, 66% include the importance of the disease, 92.9% include the symptoms of the disease, 88.7% include the treatment of the disease, 78% include the signs of the disease, 77.3% include the mechanism of the disease It has been determined that the websites examined within the scope of the research have high global quality score (GQS) and APR and are enriched with images to a large extent. Abbreviations: APR = Alexa popularity rank, ARI = automated readability index, CLI = Coleman-Liau Index, FKGL = FleschKincaid Grade Level, FRES = Flesch reading ease, GQS = global quality score, Gunning FOG = Gunning Fog Index, LWF = Linsear Write Formula, SMOG = simple measure of gobbledygookÖğe Evaluation of nosocomial infections and related risk factors in a neurosurgery intensive care unit(E-Century Publishing Corp, 2016) Erayman, Ibrahim; Erdi, Fatih; Kalkan, Erdal; Karatas, Yasar; Kaya, Bulent; Keskin, Fatih; Izci, Emir K.Nosocomial infections (NIs) cause increased morbidity, mortality, and hospital costs, particularly in intensive care units. Neurosurgery intensive care units (NICUs) differ from other intensive care units in several respects. The aim of this study was to examine NIs and related risk factors in our NICU. NICU records and the database of our hospital's infection control team from January 2010 to December 2014 were reviewed retrospectively. Type of NI and associated risk factors were subjected to statistical analysis. Demographic data, risk factors for NI development, presentation, length of NICU stay, and Glasgow Coma Scale (GCS) score were recorded and reviewed. One hundred and eighteen NI episodes were detected in 115 of 820 patients. The mean age of the patient population was 42.4 years (range 16-74 years). Of the infected patients, 55 were female and 60 were male. The mean length of hospital stay of these patients was 38.4 +/- 21.8 days. The most common type of NI was ventilator-associated pneumonia, and the most frequently detected pathogen was Acinetobacter spp. GCS score, prior usage of broad-spectrum antibiotics, reoperation, mechanical ventilation, external lumbar or ventricular drainage, tracheostomy, and duration of NICU stay were significantly higher among infected patients. Univariate analysis identified low GCS score, longer NICU stay, use of mechanical ventilation, prior usage of broad-spectrum antibiotics, and reoperation as risk factors for NI.Öğe An Experimental Study: Does the Neuroprotective Effect Increase When Hypothermia Deepens After Traumatic Brain Injury?(Kowsar Publ, 2015) Girisgin, Abdullah Sadik; Kalkan, Erdal; Ergin, Mehmet; Keskin, Fatih; Dundar, Zerrin Defne; Kebapcioglu, Sedat; Kocak, SedatBackground: Experimental approaches have been promising with the use of therapeutic hypothermia after Traumatic Brain Injury (TBI) whereas clinical data have not supported its efficacy. Objectives: This study aimed to investigate whether using selective deeper brain cooling correlates with a more neuroprotective effect on Intracranial Pressure (ICP) increments following TBI in rats. Materials and Methods: Adult male Sprague-Dawley rats (mean weight = 300 g; n = 25) were subjected to brain injury using a modified Marmarou method. Immediately after the onset of TBI, rats were randomized into three groups. Selective brain cooling was applied around the head using ice packages. Intracranial Temperature (ICT) and ICP were continuously measured at 0, 30, 60, 120, and 180 minutes and recorded for all groups. Group 1 (n = 5) was normothermia and was assigned as the control group. Group 2 (n = 10) received moderate hypothermia with a target ICT of between 32 degrees C-33 degrees C and Group 3 (n = 10) was given a deeper hypothermia with a target ICT of below 32 degrees C. Results: All subjects reached the target ICT by the 30th minute of hypothermia induction. The ICT was significantly different in Group 2 compared to Group 1 only at the 120th minute (P = 0.017), while ICP was significantly lower starting from the 30th minute (P = 0.015). The ICT was significantly lower in Group 3 compared to Groups 1 and 2 starting from the 30th minute (P = 0.001 and P = 0.003, respectively). The ICP was significantly lower in Group 3 compared to Group 1 starting from 30th minute (P = 0.001); however, a significant difference in ICP between Group 3 and Group 2 was observed only at the 180th minute (P = 0.047). Conclusions: Results of this study indicate that selective brain cooling is an effective method of decreasing ICP in rats; however, the deeper hypothermia caused a greater decrease in ICP three hours after hypothermia induction.Öğe Extradural spinal chondromyxoid fibroma mimicking intradural mass lesion: MR and MR myelographic images(Elsevier Science Inc, 2015) Keskin, Fatih; Erdi, M. Fatih; Kalkan, Havva; Karatas, Yasar; Koc, Osman[Abstract Not Availabe]Öğe Girişimsel İşlemler İçin Sakral Kanal ve Hiatusun Çok Kesitli Bilgisayarlı Tomografi ile Morfometrik Analizi(2015) Kılıçaslan, Alper; Keskin, Fatih; Babaoğlu, Ozan; Gök, Funda; Erdi, Mehmet Fatih; Kaya, Bülent; Özbiner, Hüseyin; Özbek, Orhan; Koç, Osman; Kaçıra, Burkay KutluhanAmAÇ: Yakın zamanda sakral kanal, omurga hastalıklarının minimal invaziv tanı ve tedavi işlemleri için "bir koridor olarak" sıkça kullanılmaya başlanmıştır. Çalışmanın amacı sakral kanalın, hiatusun ve çevre yapıların farklı yaş gurupları ve cinsiyete göre morfometrik analizlerinin çok kesitli bilgisayarlı tomografi yöntemiyle incelenmesidir. yÖNTem ve GeReÇLeR: Üç farklı yaş grubuna (20-80 yaş arasında) ayrılan 300 yetişkin (150 kadın ve 150 erkek; 20-80 yaş) hastanın multiplanar rekonstrüksiyon görüntüleri kaydedildi ve geriye dönük olarak incelendi. Sakral hiatus ve çevre yapılar ile sakral kanala ait çeşitli anatomik ölçümler yapıldı. Sakral kurvatür açısı ve lumbosakral lordotik açı kaydedildi. BuLGuLAR: Bazı olgularda hiatus yokluğu (%0,3), komplet agenezis (%1) ve kemik septum (%2,6) gibi kemik anomalilerine rastlandı. Anteroposterior (AP) Hiatus çapı olguların %5'sinde 2 mm nin altındaydı. Tüm yaş gruplarında, hiatus AP çapı ve hiatus alan ve "sakral kanal AP çapının en kısa mesafesinin" ortalaması, 60-80 yaş grubunda, 20-40 yaş grubuna göre daha kısaydı (p0,01). Sakral kanal AP çapın en küçük olduğu lokalizasyon, en çok olguların %59,2'unda S2 ve %33,9'unda S3 seviyesinde idi. Maksimum kurvatür seviyesi olguların %63,3'ün de S3 ve %26,7'sin de S2 seviyesinde idi. Sakral kürvatur açı ve lumbosakral lordotik açı sırayla 164 and 134 olarak ölçüldü. soNuÇ: Sakral yapılarda anatomik varyasyonlar sık görünür. Anatominin ayrıntılı analizi, girişimsel işlemlerin başarısını ve güvenilirliğini artırabilir.Öğe High thioredoxin reductase 1 expression in meningiomas undergoing malignant progression(Springer Japan Kk, 2015) Esen, Hasan; Feyzioglu, Bahadir; Erdi, Fatih; Keskin, Fatih; Kaya, Bulent; Demir, Lutfi SaltukThioredoxin (Trx) is a redox active protein that regulates several physiological and biochemical functions, such as growth, apoptosis and cellular defense. The function of Trx itself is regulated by thioredoxin reductase (TrxR). This study was designed to determine the expression of TrxR1 in meningioma tissues of different World Health Organization grades (grade I-III). Meningioma tissues were extracted from the histopathological specimens of 29 patients. These samples included seven histologically normal meningeal tissues that served as a control group and 12 grade I, 12 grade II and 5 grade III meningioma samples. TrxR1 expression was evaluated using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunostaining. The proliferative and apoptotic indices of the specimens were investigated by Ki-67 immunostaining and TUNEL assay, respectively. TrxR1 expression, as assessed by qRT-PCR, increased significantly with meningioma grade (p < 0.001). The immunostaining intensity of TrxR1 increased significantly with meningioma grade (p < 0.001). Ki-67 index values increased significantly in accordance with grade progression (p < 0.001). The apoptotic index values were not significantly different in any group (p > 0.05). Trx system seems to be involved in the malignant progression of meningiomas. Further, large studies are required to elucidate the exact role of this system.Öğe Increased expression of ubiquitin C-terminal hydrolase L1 in astrocytomas of ascending grades(Lippincott Williams & Wilkins, 2023) Izci, Emir Kaan; Keskin, Fatih; Erdi, Fatih; Kaya, Bulent; Karatas, Yasar; Feyzioglu, Bahadir; Findik, SiddikaBackground:The ubiquitin-proteasome pathway controls the monitoring and degradation of important proteins and is involved in several cellular processes, such as development, differentiation, and transcriptional regulation. Recent evidence has shown that ubiquitin carboxy-terminal hydrolase-L1 (UCH-L1), a member of the deubiquitinating enzyme family that removes ubiquitin from protein substrates, is overexpressed in many types of cancer. Aim:This study thus examined the expression of UCH-L1 in human astrocytoma tissues. Material and methods:Formalin-fixed, paraffin-embedded astrocytoma samples were obtained from 40 patients, after which histopathological examination, typing, and grading were performed. The study group included 10 histologically normal brain tissues, which served as the control group, and 10 WHO grade II, 10 WHO grade III, and 10 WHO grade IV (glioblastoma) samples. Normal brain tissue samples were obtained from the histologically normal, non-tumoral portion of the pathology specimens. UCH-L1 expression was evaluated using quantitative reverse transcription-polymerase chain reaction and immunohistochemistry. Results:Astrocytoma tissues exhibited higher UCH-L1 expression compared to the control group. UCH-L1 overexpression increased significantly together with the increase in astrocytoma grades (from II to IV). Conclusion:UCH-L1 could be a good diagnostic and therapeutic marker for determining astrocytoma development and progression.
