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Öğe Agomelatine has relaxant effect on isolated rat thoracic aorta through nitric oxide dependent mechanism(Wiley-Blackwell, 2015) Solak, H.; Koca, Ozen R.; Nurullahoglu-Atalik, K. E.; Kutlu, S.[Abstract Not Availabe]Öğe Cilostazol enhances atorvastatin-induced vasodilation of female rat aorta during aging(Akademiai Kiado Zrt, 2017) Nurullahoglu-Atalik, K. E.; Kutlu, S.; Solak, H.; Koca, R. OzenStatins have cholesterol-independent effects including an increased vascular nitric oxide activity and are commonly used by patients with cardiovascular disease. Such patients frequently have cardiovascular diseases, which may be treated with cilostazol, a platelet aggregation inhibitor. This study was designed to investigate whether combined use of cilostazol would increase the inhibitory effect of statin on vascular smooth muscle and how maturation would affect these responses. Female Wistar rats, aged 3-4 months (young) and 14-15 months (adult), were sacrificed by cervical dislocation and the thoracic aorta was dissected and cut into 3- to 4-mm-long rings. The rings were mounted under a resting tension of 1 g in a 20-ml organ bath filled with Krebs-Henseleit solution. Rings were precontracted with phenylephrine (10(-6) M), and the presence of endothelium was confirmed with acetylcholine (10(-6) M). Then, the concentration-response curves were obtained for atorvastatin alone (10(-10) to 3 x 10(-4) M; control) and in the presence of cilostazol (10(-6) M) in young and adult rat aortas. This experimental protocol was also carried out in aorta rings, which had been pretreated with NG-nitro-L-arginine methyl ester (L-NAME, 10(-4) M). Atorvastatin induced concentration-dependent relaxations in young and adult rat thoracic aorta rings precontracted with phenylephrine. The pIC(50) value of atorvastatin was significantly decreased in adult rat aortas. In addition, pretreatment of aortas with cilostazol enhanced the potency of atorvastatin in both young and adult aortas. Incubation with L-NAME did not completely eliminate the relaxations to atorvastatin in the presence of cilostazol. These results suggest that combined application of cilostazol with atorvastatin was significantly more potent than atorvastatin alone. Combined drug therapy may be efficacious in delaying the occurrence of cardiovascular events.Öğe Cilostazol enhances atorvastatin-induced vasodilation of female rat aorta during aging(Akademiai Kiado Zrt, 2017) Nurullahoglu-Atalik, K. E.; Kutlu, S.; Solak, H.; Koca, R. OzenStatins have cholesterol-independent effects including an increased vascular nitric oxide activity and are commonly used by patients with cardiovascular disease. Such patients frequently have cardiovascular diseases, which may be treated with cilostazol, a platelet aggregation inhibitor. This study was designed to investigate whether combined use of cilostazol would increase the inhibitory effect of statin on vascular smooth muscle and how maturation would affect these responses. Female Wistar rats, aged 3-4 months (young) and 14-15 months (adult), were sacrificed by cervical dislocation and the thoracic aorta was dissected and cut into 3- to 4-mm-long rings. The rings were mounted under a resting tension of 1 g in a 20-ml organ bath filled with Krebs-Henseleit solution. Rings were precontracted with phenylephrine (10(-6) M), and the presence of endothelium was confirmed with acetylcholine (10(-6) M). Then, the concentration-response curves were obtained for atorvastatin alone (10(-10) to 3 x 10(-4) M; control) and in the presence of cilostazol (10(-6) M) in young and adult rat aortas. This experimental protocol was also carried out in aorta rings, which had been pretreated with NG-nitro-L-arginine methyl ester (L-NAME, 10(-4) M). Atorvastatin induced concentration-dependent relaxations in young and adult rat thoracic aorta rings precontracted with phenylephrine. The pIC(50) value of atorvastatin was significantly decreased in adult rat aortas. In addition, pretreatment of aortas with cilostazol enhanced the potency of atorvastatin in both young and adult aortas. Incubation with L-NAME did not completely eliminate the relaxations to atorvastatin in the presence of cilostazol. These results suggest that combined application of cilostazol with atorvastatin was significantly more potent than atorvastatin alone. Combined drug therapy may be efficacious in delaying the occurrence of cardiovascular events.Öğe Cilostazol-induced relaxation of calf cardiac vein and coronary artery during cooling(Comenius Univ, 2013) Nurullahoglu-Atalik, K. E.; Nurullahoglu, Z. U.; Kilic, M.; Aribas, A.Objective: At present very little is known about the role of endothelial nitric oxide (NO) in the effects of temperature on vascular reactivity. The aim of the present study is to evaluate the influence of cooling (to 28 degrees C) on the vasodilatation induced by cilostazol(10(-9)-3x10(-4)M) on carbachol (10(-6))-precontracted calf cardiac vein and coronary artery and the role of NO in these effects. Materials and methods: Ring preparations of great cardiac vein and the anterior interventricular branch of left coronary artery were used. Results: Cilostazol produced concentration-dependent relaxation of calf cardiac vein and coronary artery rings precontracted with carbachol. During cooling, the pIC(50) values, but not the maximal responses to cilostazol were significantly lower than at 37 degrees C in both preparations. Cooling to 28 degrees C in the presence of N-G-nitro-L-arginine methyl ester (L-NAME, 10(-4) M) did not modify the effect of temperature both in cardiac vein and coronary artery. These results demonstrate for the first time that cooling-induced changes of cilostazol in calf cardiac vein and coronary artery are independent of NO (Tab. 2, Fig. 3, Ref. 32). Full Text in PDF www.elis.sk.Öğe Donepezil-induced response of Spirulina supplemented rat urinary bladder(Comenius Univ, 2013) Nurullahoglu-Atalik, K. E.; Okudan, N.; Gokbel, H.; Nurullahoglu, Z. U.Objective: At present, very little is known about the effects of donepezil on vascular reactivity. The aim of the present study was to evaluate the responses of rat urinary bladder to donepezil (10(-10)-3x10(-4) M) and the role of Spirulina supplementation in these effects. Material and methods: Animals were divided into the two groups of six animals in each group. The first group received only distilled water daily as vehicle for six weeks and served as the control. The second group received Spirulina 750 mg kg (-1) orally, daily for six weeks and served as the spirulina group. Preparations of rat urinary bladder were used from both groups. Results: Donepezil produced concentration dependent relaxation of rat urinary bladder preparations pre-contracted with KCl. The pIC(50) value, but not the maximal response of donepezil, was significantly lower (p<0.05) in the Spirulina supplemented group. Conclusions: These results demonstrated for the first time that spirulina treatment can affect urinary bladder activity (Fig. 1, Ref. 20). Full Text in PDF www.elis.sk.Öğe Moderate hypothermia attenuates ?1-adrenoceptor-mediated contraction in human varicose spermatic vein: The role of nitric oxide (Short communication)(Akademiai Kiado Rt, 2016) Nurullahoglu-Atalik, K. E.; Cenker, A.The effects of moderate hypothermia (28 degrees C) on the response of human varicose spermatic vein to alpha(1)-adrenoceptor agonist phenylephrine and the role of endothelial nitric oxide (NO) in these effects were studied. Concentration-response curves for phenylephrine (10(-9) to 3 x 10(-4) M) were recorded in rings with and without endothelium at 37 and 28 degrees C. To further analyze the role of NO, in the response to phenylephrine during hypothermia, the effects of this agonist in the presence of N-G-nitro-L-arginine methyl ester (10(-4) M) were also determined. Under every condition tested, phenylephrine produced a marked, concentration-dependent contraction. Sensitivity of intact veins to the agonist was consistently lower at 28 degrees C than at 37 degrees C. There was no significant difference in phenylephrine response at 28 and 37 degrees C in vessels without endothelium but at 28 degrees C veins without endothelium showed a higher sensitivity than intact veins to phenylephrine. The sensitivity of veins with and without endothelium to nitroprusside (10(-9) to 3 x 10(-3) M) was significantly decreased during hypothermia, and endothelium removal did not affect the relaxation to this nitrovasodilator. These results suggest that moderate hypothermia decreases the sensitivity of human varicose spermatic vein to phenylephrine probably by increasing the availability of endothelial NO.Öğe Moderate hypothermia attenuates ?1-adrenoceptor-mediated contraction in human varicose spermatic vein: The role of nitric oxide (Short communication)(Akademiai Kiado Rt, 2016) Nurullahoglu-Atalik, K. E.; Cenker, A.The effects of moderate hypothermia (28 degrees C) on the response of human varicose spermatic vein to alpha(1)-adrenoceptor agonist phenylephrine and the role of endothelial nitric oxide (NO) in these effects were studied. Concentration-response curves for phenylephrine (10(-9) to 3 x 10(-4) M) were recorded in rings with and without endothelium at 37 and 28 degrees C. To further analyze the role of NO, in the response to phenylephrine during hypothermia, the effects of this agonist in the presence of N-G-nitro-L-arginine methyl ester (10(-4) M) were also determined. Under every condition tested, phenylephrine produced a marked, concentration-dependent contraction. Sensitivity of intact veins to the agonist was consistently lower at 28 degrees C than at 37 degrees C. There was no significant difference in phenylephrine response at 28 and 37 degrees C in vessels without endothelium but at 28 degrees C veins without endothelium showed a higher sensitivity than intact veins to phenylephrine. The sensitivity of veins with and without endothelium to nitroprusside (10(-9) to 3 x 10(-3) M) was significantly decreased during hypothermia, and endothelium removal did not affect the relaxation to this nitrovasodilator. These results suggest that moderate hypothermia decreases the sensitivity of human varicose spermatic vein to phenylephrine probably by increasing the availability of endothelial NO.Öğe The role of nitric oxide on the responses of curcumin-treated rat aorta(Comenius Univ, 2012) Nurullahoglu-Atalik, K. E.; Gokbel, H.; Oz, M.; Okudan, N.; Belviranli, M.; Esen, H.Objectives: The aim of the present study was to evaluate the influence of endothelial nitric oxide (NO) on the vascular responses of curcumin-treated rats. Methods: The experimental groups included the control and curcumin-treated (200 mg/kg/day, p.o., for 4 weeks) group. The concentration response curves to receptor-dependent agent 5-hydroxytryptamine (5-HT; 10(-9)-3x10(-4) M) and receptor-independent agent potassium chloride (KCl; 5-100 mM) were observed. Results: The concentration response curves to 5-HT and KCl shifted to the right and the maximal response was significantly decreased in the curcumin-treated rat aortas. A pretreatment of rings with L-NAME (a NOS inhibitor, 10(-4) M) increased both the sensitivity and maximal response to only 5-HT. No apparent histological changes were demonstrated in smooth muscle and connective tissue layers in the aortas of the control and curcumin-treated rat preparations. Conclusion: The results of the present study suggest that NO release from endothelial cells modulates curcumin-treated rat aorta responses to 5-HT, but not to KCl (Tab. 2, Fig. 4, Ref. 25). Full Text in PDF www.elis.sk.Öğe Rosuvastatin-induced responses in calf cardiac vein(Comenius Univ, 2015) Nurullahoglu-Atalik, K. E.; Oz, M.; Shafiyi, A.OBJECTIVE: The effects of Rho-kinase inhibitors on vasodilatation induced by 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor rosuvastatin (10(-9)-10(-4)M) on 5-HT-precontracted calf cardiac vein and the role of endothelium in these effects were analyzed. MATERIAL AND METHODS: Cardiac vein ring preparations were suspended in organ baths containing 25 ml of Krebs Henseleit solution, maintained at 37 degrees C and continuously gassed with 95 % O-2-5 % CO2. At the end of the resting period, the cardiac vein preparations were contracted with 10(-6) M 5-HT. After the contraction had reached a steady state, rosuvastatin was added to the organ bath cumulatively (10(-9)-10(-4)M). RESULTS: Rosuvastatin relaxed the cardiac vein rings in general while the degree of relaxation was greater in those with endothelium and lower in those without it. HA1077 [1-(5-isoquinolinesulfonyl)-homopiperazine] (Fasudil, 10(-6) M) and Y-27632 [(+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl) cyclohexane carboxamide dihydrochloride] (10(-6) M) incubation increased the rosuvastatin-induced relaxation only in the presence of endothelium. CONCLUSIONS: The results demonstrate for the first time that in calf cardiac vein, rosuvastatin induced endothelium-dependent relaxations while Rho-kinase inhibition increased these relaxations in the presence of endothelium layer (Fig. 3, Ref. 44). Text in PDF www.elis.sk.
