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Öğe Bacterial contamination of coins obtained from school canteen and green market(Taylor & Francis Ltd, 2019) Yontem, Mustafa; Doyuk, Sabire A.; Erci, Fatih; Erdogdu, Behic S.[Abstract Not Availabe]Öğe The Evaluation of the Prevalence of Metabolic Syndrome and Lipid Profile According to Body Mass Index in Obese Women(Duzce Univ, 2015) Korkut, Yasemin; Kocak, Fatma Emel; Kilit, Turkan Pasali; Arikan, Inci; Teksen, Yasemin; Yontem, Mustafa; Birgul, MehmetObjective: We aimed to evaluate the prevalence of metabolic syndrome (MS) and lipid profile according to body mass index (BMI) in obese women. Methods: BMI and blood parameters were compared in 102 patients who applied to the clinic owing to the failure to lose weight complaint between December 2013 and January 2014. They were classified according to BMI; 30,0-34,9 kg/m(2) as 1st degree obese, 35,0-39,9 kg/m(2) as 2nd degree obese, and >= 40,0 kg/m(2) as 3rd degree (morbid) obese. Results: All the patients were women. Their age average was 45,01 +/- 11,0 yrs. 36,3% of these patients were at 1.st degree, 5% of them were at 2.st degree and 39,2% of them were at 3.st degree of obesity. We found the prevalence of MS as 56,9%. According to their degree of obesity increased prevalence of MS, the MS was increased with increasing age (p<0,05). With the evaluation of patients blood parameters according to obesity degrees we found a meaningful increase in blood glucose levels avarage (p=0,048), HOMA-IR (p=0,009) and in insulin levels (p=0,031) in upper levels of obesity. Whereas, while the obesity degrees were increasing there were in increase in triglycerides, total cholesterol, HDL-cholesterol, LDL-cholesterol and HbA1c levels but the rise was statically meaningless. Conclusion: In present study we found that as BMI was increasing the MS was increasing also. Although we didn't find a significant relationship between BMI increment and lipid profile in the women with MS but there was a direct proportion. Besides we found that as the BMI was increasing, blood glucose, HOMA-IR, insulin levels were increasing also. As a conclusion if the women and their family change their eating habits and life styles, the whole family will be affected positively and it is clear that the development of obesity, dsylipidemia and MS will decrease.Öğe Plant extract mediated silver nanoparticles by concentrated sunlight and their antibacterial and cytotoxic activities(Taylor & Francis Inc, 2022) Kutuk, Yagmur; Yontem, Mustafa; Erci, Fatih; Esirgenler, Busra; Isildak, Ibrahim; Totu, Eugenia EftimieHere we report the synthesis of silver nanoparticles (AgNPs) under concentrated sunlight by using a newly designed solar system. In the study, AgNPs synthesized by using different plant extracts of Ginko biloba (Gb), Alpinia officinarum (Ao), Foeniculum vulgare (Fv) and Cassia angustifolia (Ca) were characterized by different techniques. The antibacterial activity of the AgNPs was determined by agar diffusion assay. TEM analysis revealed the spherical nanoparticles with average size of 26, 23,44 and 32 nm in diameter for Gb, Ao, Fv and Ca AgNPs, respectively. Also, we found that the AgNPs were not cytotoxic at the concentration of 2.5 mu g/mL. We also revealed that Ca AgNPs showed the highest antibacterial activity against S. aureus with inhibition zone of 15.27 mm. All in all, the results indicated that the formation of AgNPs was achieved in less than 1 h without any energy consumption in the solar system with concentrated sunlight.Öğe Rapid determination of adulteration of clove essential oil with benzyl alcohol and ethyl acetate: Towards quality control analysis by FTIR with chemometrics(Elsevier, 2022) Tarhan, Ismail; Bakir, Muhammed Rasit; Kalkan, Oktay; Yontem, Mustafa; Kara, HuseyinIn this study, Fourier transform infrared (FTIR) spectroscopy in tandem with chemometrics was used for the discrimination and quantification of the adulterants, such as benzyl alcohol (B) and ethyl acetate (E) in clove essential oil (CO). Different multivariate models with various spectral derivatization methods were developed and their analysis abilities the adulterants were compared using statistical quality parameters. To discriminate the adulterations thanks to the FTIR data, 130 chemometric models were built by principal component analysis (PCA) algorithm. The statistical performances of the PCA models developed were evaluated by the number of samples outside of explained variance (95 %) and eigen value. To quantify the adulterant concentrations in CO samples, 117 partial least squares (PLS) regression models employing the FTIR data were developed. To find out the best PLS model, the root mean square error of prediction (RMSEP), and root mean square error of cross-validation (RMSECV) were mainly used. Root mean square error of calibration (RMSEC) and R-square were also evaluated. The best discrimination results were achieved using 1st derivative spectra in the region 3500-3100 cm(-1) (SRB1) and 2nd derivative spectra in the region 1077-1008 cm(-1) (SRE3) for the adulterants of B and E, respectively. The best PLS calibration results were obtained from the combinations of the normal spectra in the regions 3500-3100 cm(-1), 1027-993 cm(-1), and 756-569 cm(-1) (SRBC) and SRE3 for the quantification of B and E, respectively. The results of the study indicated that FTIR with chemometrics could be used for simultaneously discrimination and quantification of the adulterants of B and E in COs without using any toxic chemicals or pretreatments.Öğe SERUM LEVELS OF OXIDATIVE STRESS MARKERS IN SUBCLINICAL AND OVERT HYPOTHYROIDISM VERSUS CONTROL GROUP IN POPULATION OF KUTAHYA CITY, TURKEY(Gomal Medical Coll, 2021) Yontem, Mustafa; Arslan, Serap; Erdogdu, Behic Selman; Kocak, Fatma EmelBackground: Overproduction of oxygen-related free radicals and inadequate antioxidant defense are critical in hypothyroidism, as they might lead to future life-threatening diseases. The objectives of this study were to compare serum levels of oxidative stress markers; total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), paraoxonase-1 (PON-1), and arylesterase (ARYL) in patients with subclinical hypothyroidism (SH) and overt hypothyroidism (OH) versus healthy controls in population of Kutahya city, Turkey. Materials & Methods: This cross-sectional study was conducted at Department of Biochemistry, Faculty of Medicine, Kutahya University of Health Sciences, Kutahya, Turkey, from July to December, 2016. Three study groups of 45 each were formed as healthy controls, patients with SH and OH. Serum levels of TOS, TAS, OSI, PON-1, and ARYL were five research variables with ratio scale, but skewed, so described by median and IQR with 95% Cl. Kruskal-Wallis and multiple comparison tests were used for hypotheses testing. Results: Serum levels of TOS and OSI were higher in SH group (p-values 0.032; <.0001; respectively) and OH group (p-values <.0001; <.0001, respectively) than healthy controls, while serum levels of TAS, PON-1 and ARYL were lower in SH group (p-values <.0001; <.0001; <.0001, respectively) and OH group (p-values <.0001; <.0001; <.0001, respectively) than control group. Conclusion: Our study showed that serum levels of TOS and OSI were higher in subclinical and overt hypothyroidism than in healthy controls; while serum levels of TAS, PON-1, and ARYL were lower in subclinical and overt hypothyroidism than in healthy controls.Öğe SERUM LEVELS OF OXIDATIVE STRESS PARAMETERS IN POSTMENOPAUSAL VERSUS FERTILE WOMEN OF KUTAHYA CITY, TURKEY(Gomal Medical Coll, 2021) Erdogdu, Behic Selman; Yontem, Mustafa; Kocak, Fatma Emel; Yazar, HayrullahBackground: Decreased concentration of both oestrogen hormone and vitamin D in postmenopausal period may lead to oxidative stress, lipid peroxidation, dyslipidaemia, osteoporosis, and cardiovascular diseases. The objectives of this study were to determine and compare the serum levels of oxidative stress parameters; TOS, TAS, OSI, PON-1, ARYL in postmenopausal and fertile women of Kutahya city, Turkey. Materials & Methods: This cross-sectional study was conducted at Department of Biochemistry, Faculty of Medicine, Kutahya University of Health Sciences, Kutahya, Turkey from July, 2016 to December, 2016. Kutahya is a city in western Turkey, with population of 237,804 as per 2011 estimates. Two samples were drawn from this population with consecutive technique. Study group included 40 menopausal women, while control group included 40 healthy fertile women. After overnight fasting, venous blood samples were collected, centrifuged, aliquoted into a polystyrene tube, and aliquots were stored at -80 degrees C until measurement for total oxidant status (TOS), total antioxidant status (TAS), paraoxonase-1 (PON-1), and arylesterase (ARYL) measurements were made. Levels of TOS, TAS, OSI, PON-1, ARYL were five research variables on ratio scale. `Normal' data were described by mean, range and SD and skewed data by median, Q1, Q3 and IQR with 95% confidence intervals. For normal data, independent-samples t-test and for skewed data Mann-Whitney U test was used for hypotheses testing. Results: Serum total oxidant status (TOS) and oxidative stress index (OSI) levels were significantly higher (p-value.015 &.003 respectively), and serum paraoxonase-1 (PON-1) and arylesterase (ARYL) levels were significantly lower in postmenopausal than fertile women (p-value <.0001 &.0005 respectively), with no statistical difference for serum total antioxidant status (TAS) levels between the two groups (p-value.186). Conclusion: Postmenopausal period is associated with oxidative stress and decreased antioxidant defence. HDL-c-dependent PON-1 and ARYL activities are also reducing due to a decrease of HDL-c with menopause. Elevated levels of TOS and OSI, decreased levels of PON-1 and ARYL may lead to various life-threatening diseases such as cardiovascular disorders or cancer.Öğe Synthesis of biologically active copper oxide nanoparticles as promising novel antibacterial-antibiofilm agents(Taylor & Francis Inc, 2020) Erci, Fatih; Cakir-Koc, Rabia; Yontem, Mustafa; Torlak, EmrahIn this study, we aimed to synthesize copper oxide nanoparticles (CuONPs) mediated by plant extract in an environmentally friendly way and to reveal their potential biological activities. Here we synthesized CuONPs by using different concentrations of aqueous leaf extract of Thymbra spicata at 80 degrees C to obtain Ts1CuONPs and Ts2CuONPs. Biosynthesized nanoparticles were characterized by using UV-Vis, AFM, FTIR, SEM-EDS, TEM, DLS and zeta potential analysis. The antibacterial activity of the nanoparticles was determined by calculation of the inhibition zone and minimum inhibitory concentration against selected bacterial strains. Moreover, the antioxidant activity of the as-synthesized nanoparticles was evaluated based on DPPH radical scavenging activity. The results indicate that the as-synthesized NPs have an average size of 26.8 and 21 nm for Ts1CuONPs and Ts2CuONPs, respectively. The formed CuONPs have more antibacterial action on gram-positive bacteria compared to gram-negative bacteria. In addition, CuONPs demonstrated good inhibition activity against biofilm formation of Staphylococcus aureus (S. aureus). Furthermore, the results showed that the smaller size of the CuONPs caused the higher cytotoxicity on L929 mouse fibroblast cells. The as-synthesized CuONPs exhibit antibacterial and antibiofilm potential against S. aureus, indicating that they may be attractive candidates to use in future therapeutic applications.
