The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Makerere Univ, Coll Health Sciences,Sch Med
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: Pancreatic cancer does not show any symptoms in the early period and metastatic process is already passed when the diagnosis is done. Therefore, in the battle with pancreatic cancer, novel treatment strategies, particularly antiinva-sive and antimetastatic strategies, are needed. The cytotoxic and anticancer effects of juglone and sodium selenite (NaSe) have been showed in various cancer cells. Objectives: In this study, it is aimed to investigate the synergistic effects of juglone and selenium on PANC-1 and BxPC-3 pancreatic cancer cells. Methods: Antimetastatic effects of juglone-NaSe were carried out by adhesion and invasion assays and the genes and pro-tein expressions. Expression analysis of the CDH1, ITGB3 and COL4A3 genes and their proteins E-cadherin, beta 3 integrin and tumstatin which play role in metastasis and angiogenesis processes, were done by qPCR and immunohistochemical analysis, respectively. Results: Study findings have provided evidences that the juglone-selenium has a cytotoxic and dose dependent suppressive effect on invasion and metastasis in PANC-1 and BxPC-3 cells. Conclusion: The juglone-NaSe has the potential to be a promising agent especially to inhibit invasion and metastasis in pan-creatic cancer treatment. However, more in depth studies are needed to more clearly demonstrate the effects of juglone-se-lenium.
Açıklama
Anahtar Kelimeler
Pancreatic Cancer Cell Lines, Juglone-Selenium, Invasion, Metastasis
Kaynak
African Health Sciences
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
22
Sayı
2
