Pulmoner ve ekstrapulmoner tüberküloz hastalarında CCL1 rs159294 T/A gen polimorfizminin araştırılması
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Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Giriş: Bu çalışmada amaç, pulmoner ve ekstrapulmoner tüberküloz hastalarındaki, CCL1 rs159294 T/A polimorfizminin tüberküloza yakalanma riski oluşturup oluşturmadığını ortaya koymaktır.
Materyal ve Metod: Çalışmamızda Elazığ ili yöresinde Fırat Üniversitesi Tıp Fakültesi Göğüs Hastalıkları Polikliniğine başvuran, tüberküloz teşhisi konulmuş 160 hasta ve 160 sağlıklı bireyden oluşan kontrol grubuna ait kişilerden periferal kan
örnekleri alınarak EDTA içeren tüplere 2 cc olacak şekilde konulmuştur. Bu kan örneklerinden DNA izolasyonu gerçekleştirilerek CCL1 rs159294 T/A polimorfizmi PCR-RFLP analizi yapılarak belirlenmiştir.
Bulgular: CCL1 rs159294 T/A polimorfizmi için 160 tüberkülozlu hastanın 98 (%61.3)’inde TT genotipi, 58 (%36.3)’inde TA
genotipi, 4 (%2.5)’ünde de AA genotipi, 71 pulmoner tüberkülozlu hastanın 50 (%70.4)’sinde TT genotipi, 20 (%28.2)’sinde
TA genotipi, 1 (%1.4)’inde de AA genotipi, 89 ekstrapulmoner tüberkülozlu hastanın 48 (%53.9)’inde TT genotipi, 38
(%42.7)’inde TA genotipi, 3 (%3.4)’ünde de AA genotipi tespit edilmiştir. Kontrol grubunda ise 160 sağlıklı bireyin 100
(%62.5)’ünde TT genotipi, 58 (%36.3)’inde TA genotipi, 2 (%1.3)’sinde de AA genotipi belirlenmiş olup, istatistiksel olarak
anlamlı bir farklılık saptanamamıştır.
Sonuç: CCL1 rs159294 T/A polimorfizmi, popülasyonumuz da tüberküloz hastalığına yatkınlık oluşturmamaktadır
Introduction: The purpose of this study is to reveal whether CCL1 rs159294 T/A polymorphism in pulmonary and extrapulmonary tuberculosis patients pose a risk to catch tuberculosis or not. Materials and Methods: In the study, peripheral blood samples from the control group, which includes 160 patients, who consulted to Fırat University Faculty of Medicine, Pulmonology Policlinic in Elazığ province and who were diagnosed with tuberculosis; and 160 healthy individuals, were taken and put into tubes containing EDTA. Each tube contained 2 cc blood samples. DNA isolation was made from these blood samples and CCL1 rs159294 T/A polymorphism was defined with PCR-RFLP analysis. Results: For CCL1 rs159294 T/A polymorphism, TT genotype was found in 98 (61.3%) patients, TA genotype was found in 58 (36.3%) patients, AA genotype was found in 4 (2.5%) patients among 160 patients with tuberculosis; and TT genotype was found in 50 (70.4%) patients, TA genotype in 20 (28.2%) patients, AA genotype was found in 1 (1.4%) patient among 71 patients with pulmonary tuberculosis; TT genotype was found in 48 (53.9%) patients TA genotype was found in 38 (42.7%) patients and AA genotype was found in 3 (3.4%) patients among 89 extrapulmonary tuberculosis patients. And in control group, among 160 healthy individuals, TT genotype was found in 100 (62.5%) individuals, TA genotype was found in 58 (36.3%) individuals, AA genotype was found in 2 (1.3%) individuals and no statistically significant difference was found. Conclusion: CCL1 rs159294 T/A polymorphism do not form an inclination to tuberculosis in our population
Introduction: The purpose of this study is to reveal whether CCL1 rs159294 T/A polymorphism in pulmonary and extrapulmonary tuberculosis patients pose a risk to catch tuberculosis or not. Materials and Methods: In the study, peripheral blood samples from the control group, which includes 160 patients, who consulted to Fırat University Faculty of Medicine, Pulmonology Policlinic in Elazığ province and who were diagnosed with tuberculosis; and 160 healthy individuals, were taken and put into tubes containing EDTA. Each tube contained 2 cc blood samples. DNA isolation was made from these blood samples and CCL1 rs159294 T/A polymorphism was defined with PCR-RFLP analysis. Results: For CCL1 rs159294 T/A polymorphism, TT genotype was found in 98 (61.3%) patients, TA genotype was found in 58 (36.3%) patients, AA genotype was found in 4 (2.5%) patients among 160 patients with tuberculosis; and TT genotype was found in 50 (70.4%) patients, TA genotype in 20 (28.2%) patients, AA genotype was found in 1 (1.4%) patient among 71 patients with pulmonary tuberculosis; TT genotype was found in 48 (53.9%) patients TA genotype was found in 38 (42.7%) patients and AA genotype was found in 3 (3.4%) patients among 89 extrapulmonary tuberculosis patients. And in control group, among 160 healthy individuals, TT genotype was found in 100 (62.5%) individuals, TA genotype was found in 58 (36.3%) individuals, AA genotype was found in 2 (1.3%) individuals and no statistically significant difference was found. Conclusion: CCL1 rs159294 T/A polymorphism do not form an inclination to tuberculosis in our population
Açıklama
Anahtar Kelimeler
Pulmoner tüberküloz, Ekstrapulmoner tüberküloz, CCL1, Polimorfizm, Pulmonary tuberculosis, Extrapulmonary tuberculosis, Polymorphism
Kaynak
Tüberküloz ve Toraks
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
61
Sayı
3
Künye
Şenli Kara, E., Özdemir, F. A., Erol, D., Yüce, H., Konar, V., Bulut, F., Deveci, F. (2013). Pulmoner ve ekstrapulmoner tüberküloz hastalarında CCL1 rs159294 T/A gen polimorfizminin araştırılması. Tüberküloz ve Toraks, 61, 3, 200-208.
