Normal ve hiperkolesterolemik tavşanlara vitamin D ilavesinin TAS, TOS, MDA ve paraoksonaz düzeylerine etkileri
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Dosyalar
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Necmettin Erbakan Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Çalışmamızda normal ve hiperkolesterolemik erkek tavşanlara D vitamini ilavesinin total antioksidan seviye (TAS), total oksidan seviye (TOS), malondialdehid (MDA), ve paraoksonaz-1 (PON-1) değerleri üzerine etkilerini araştırmak amaçlandı. Hayvanlar rastgele dört gruba ayrıldı. 1. Kontrol (n: 6), 2. D vitamini ilave (VitD) (n: 8), 3. % 0.5 Hiperkolesterolemi+D vitamini (HC+VitD) (n: 8), 4. % 0.5 Hiperkolesterolemili (HC) (n: 8). 1. ve 2. grup normal tavşan pelet yemiyle, 3. ve 4. grup önceden hazırlanmış olan % 0.5'lik kolesterol yemi ile 8 hafta süresince beslenildi. 2. ve 3. gruba oral yoldan günlük D vitamini 300 IU/kg olarak 8 hafta boyunca verildi. Tüm tavşanlardan deney öncesi ve 2 haftada bir kan örnekleri alındı. 8. haftanın sonunda kan ve karaciğer örnekleri alındı. Total kolesterol, HDL-kolesterol (HDL-C), LDL-kolesterol (LDL-C), trigliserit (TG), kalsiyum ve 25OH D vitamini seviyeleri alınan kanlardan bakıldı. TAS, TOS, MDA ve PON-1 ölçümleri ise hem alınan kanlardan hem de karaciğer dokularından çalışıldı. HC+VitD grubuna göre; HC grubu serum total kolesterol seviyesi ortalamasındaki artış gruplar arası karşılaştırıldığında anlamlı bulundu (p<0.05). D vitamini grubu HDL-C seviyesi ortalaması grup içi karşılaştırıldığında sonuçlar anlamlı bulundu (p<0.05). HC+VitD grubuna göre; HC grubu serum OSI seviyesi ortalamasındaki artış gruplar arası karşılaştırıldığında anlamlı bulundu (p<0.05). Serum PON-1 aktivite değerleri HC+VitD grubuna göre; HC grubu serum PON-1 aktivite değerleri ortalamasındaki azalış gruplar arası karşılaştırıldığında anlamlı bulundu (p<0.05). Sonuç olarak bizim çalışmamızda D vitamini takviyesi yaptığımız erkek tavşanların total kolesterol, HDL-C (Bağımlı değişken), serum OSI seviyelerinde kontrollerine göre anlamlı bir azalış görüldü. Günümüzde D vitamininin ilave olarak alınması çok fazla artmıştır. Doğal yollarla alabildiğimiz D vitaminini, ilave almak gerektiğinde; günlük alınan miktarına, süresine, yaşa, cinsiyete göre dikkat etmek gerektiği düşüncesindeyiz. Çalışmamızda D vitamini her ne kadar OSI ve total kolesterolü azaltsada, aynı zamanda HDL-C ve PON-1 değerlerini de azaltmış olduğu görüldüğünden alınan miktarın fazlalığına ve süresine göre faydalı olacağı yerde zararlı etkileri ile karşılaşılabilineceği kanaatindeyiz.
This study aimed to evaluate the effect of vitamin D supplementation on total antioxidant status (TAS), total oxidant status (TOS), malondialdehyde (MDA), and paraoxonase-1 (PON-1) in values in healthy and hypercholesterolemic male rabbits. Animals were divided into four groups, randomly: 1. Control (n: 6); 2. Vitamin D (VitD) supplementation (n: 8); 3. 0.5 % hypercholesterolemic+Vitamin D (HC+VitD) (n: 8); 4. 0.5 % hypercholesterolemic (HC) (n: 8). Through 8 weeks, while 1st and 2nd groups were fed with normal rabbit pellets, 3rd and 4th groups were fed with previously prepared 0.5 % cholesterol feeds. During this process, 2nd and 3rd groups were orally administered with 300 IU/kg vitamin D for each day. Blood samples were collected just before the experiment and 2 weeks periods from all rabbits. End of the 8 weeks, blood and liver samples were collected. Total cholesterol, HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C) triglyceride (TG), calcium and 25OHD vitamin levels were measured from collected blood samples. TAS, TOS, MDA and PON-1 were measured from both blood samples and liver tissues. With respect to HC+VitD group; increase in serum total cholesterol levels of HC group is statistically significant (p<0.05) when compared between the groups. HDL-C levels of VitD groups are statistically significant (p<0.05) within the groups. Increase in serum OSI levels of HC group is statistically significant (p<0.05), when compared between the groups with respect to HC+VitD group. Decrease in HC groups serum PON-1 activity is statistically significant (p<0.05) with respect to HC+VitD group, when it is analysed between the groups. As a conclusion, in our study, it was observed that there was a significant decrease in total cholesterol, HDL-C (dependent variable), serum OSI levels of vitamin D supplemented male rabbits with respect to control group. Nowadays, vitamin D supplementation is increased excessively. Although it can be obtained from natural sources, we suggest that daily dosage, take up period, age and sex should be considered, when vitamin D supplementation is needed. In our study, although administration of vitamin D decreased the OSI and cholesterol levels, it also decreased HDL-C and PON-1 in values at the same time, therefore, we aggreed on that it can show detrimental effects where it can be beneficial based on its dosage and period.
This study aimed to evaluate the effect of vitamin D supplementation on total antioxidant status (TAS), total oxidant status (TOS), malondialdehyde (MDA), and paraoxonase-1 (PON-1) in values in healthy and hypercholesterolemic male rabbits. Animals were divided into four groups, randomly: 1. Control (n: 6); 2. Vitamin D (VitD) supplementation (n: 8); 3. 0.5 % hypercholesterolemic+Vitamin D (HC+VitD) (n: 8); 4. 0.5 % hypercholesterolemic (HC) (n: 8). Through 8 weeks, while 1st and 2nd groups were fed with normal rabbit pellets, 3rd and 4th groups were fed with previously prepared 0.5 % cholesterol feeds. During this process, 2nd and 3rd groups were orally administered with 300 IU/kg vitamin D for each day. Blood samples were collected just before the experiment and 2 weeks periods from all rabbits. End of the 8 weeks, blood and liver samples were collected. Total cholesterol, HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C) triglyceride (TG), calcium and 25OHD vitamin levels were measured from collected blood samples. TAS, TOS, MDA and PON-1 were measured from both blood samples and liver tissues. With respect to HC+VitD group; increase in serum total cholesterol levels of HC group is statistically significant (p<0.05) when compared between the groups. HDL-C levels of VitD groups are statistically significant (p<0.05) within the groups. Increase in serum OSI levels of HC group is statistically significant (p<0.05), when compared between the groups with respect to HC+VitD group. Decrease in HC groups serum PON-1 activity is statistically significant (p<0.05) with respect to HC+VitD group, when it is analysed between the groups. As a conclusion, in our study, it was observed that there was a significant decrease in total cholesterol, HDL-C (dependent variable), serum OSI levels of vitamin D supplemented male rabbits with respect to control group. Nowadays, vitamin D supplementation is increased excessively. Although it can be obtained from natural sources, we suggest that daily dosage, take up period, age and sex should be considered, when vitamin D supplementation is needed. In our study, although administration of vitamin D decreased the OSI and cholesterol levels, it also decreased HDL-C and PON-1 in values at the same time, therefore, we aggreed on that it can show detrimental effects where it can be beneficial based on its dosage and period.
Açıklama
Doktora Tezi
Anahtar Kelimeler
D vitamini, Hiperkolesterolemi, Oksidatif Stres, Paraoksonaz-1, Tavşan, Hypercholesterolemia, Oxidative Stress, Paraoxonase-1, Rabbit, Vitamin D
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Kocabaş, R. (2016). Normal ve hiperkolesterolemik tavşanlara vitamin D ilavesinin TAS, TOS, MDA ve paraoksonaz düzeylerine etkileri. (Yayımlanmamış doktora tezi). Necmettin Erbakan Üniversitesi, Sağlık Bilimleri Enstitüsü Tıbbi Biyokimya Anabilim Dalı, Konya.
