Genetic Diversity and Population Structure in Bread Wheat Germplasm from Turkiye Using iPBS-Retrotransposons-Based Markers
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study investigated the genetic diversity and population structure of 63 genotypes from Turkish bread wheat germplasm using iPBS-retrotransposons primers. The thirty-four iPBS primers produced a total of 1231 polymorphic bands, ranging from 8 (iPBS-2375) to 60 (iPBS-2381) alleles per marker, with an average number of 36.00 alleles. The polymorphism information content (PIC) per marker varied between 0.048 (iPBS 2087) and 0.303 (iPBS 2382), with an average of 0.175. The numbers of effective alleles (ne), genetic diversity of Nei (h), and Shannon's information index (I) value were calculated as 1.157, 0.95, and 0.144, respectively. The greatest genetic distance (0.164) was between Eastern Anatolia Agricultural Research Institute genotypes and cukurova Agricultural Research Institute genotypes. The unweighted pair-group method with arithmetic mean (UPGMA) dendrogram placed the 63 wheat genotypes into three clusters. The percentage of genetic diversity explained by each of the three main coordinates of the basic coordinate analysis was determined to be 44.58, 12.08, and 3.44, respectively. AMOVA (Analysis of Molecular Variance) showed that the variation within populations was 99% and that between populations was 1%. The result of genetic structure analysis suggests that the greatest value of K was calculated as 3. The F-statistic (Fst) value was determined as 0.4005, 0.2374, and 0.3773 in the first to third subpopulations, respectively. Likewise, the expected heterozygosity values (He) were determined as 0.2203, 0.2599, and 0.2155 in the first, second, and third subpopulations, respectively. According to the information obtained in the study, the most genetically distant genotypes were the G1 (Aksel 2000) and G63 (Karasu 90) genotypes. This study provided a deep insight into genetic variations in Turkish bread wheat germplasm using the iPBS-retrotransposons marker system.
Açıklama
Makale
WOS:000914297800001
WOS:000914297800001
Anahtar Kelimeler
Wheat, Germplasm, Genetic Diversity, Environmental Conditions, Genotypes, Inter-primer Binding Site, Varieties, PCoA, AMOVA
Kaynak
Agronomy-Basel
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
13
Sayı
1
Künye
Haliloğlu, K., Türkoğlu, A., Öztürk, A., Niedbala, G., Niazian, M., Wojciechowski, T., Piekutowska, M. (2023). Genetic diversity and population structure in bread wheat germplasm from Turkiye using iPBS-retrotransposons-based markers. Agronomy-Basel, 13, 1, 1-15.
