The Effects of Laser with Different Parameters and Crosshatching on Fibroblast Adhesion and Proliferation to Implant Surfaces
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Quintessence Publishing Co Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: To compare the adhesion and proliferation effect of HGF-1 cells on pure titanium disks when the surfaces are unprocessed (machined surfaces after slicing) or modified with a laser. Materials and Methods: Twenty-eight titanium disks were divided into four groups. Three surface topographies were created using an erbium fiber laser: group 1 (unidirectional application [no crosshatch]), group 2 (crosshatching in two directions), and group 3 (crosshatching in three directions). The samples in group 4 were unprocessed and served as controls. The surface roughness of samples was investigated with scanning electron microscopy (SEM) and measured with a mechanical surface profilometer. HGF-1 cell line was used to analyze the adhesion of fibroblasts on the sample surfaces. The authors used SEM, XTT, and acridine orange tests to determine the adhesion of HGF-1 onto specimen surfaces and the cell morphology after incubation for 72 hours. XTT results and surface roughness values for all specimens were analyzed using the Kruskal-Wallis test. Results: Ra values of group 1 to group 4 (control) were 1.13, 0.26, 0.38, and 0.19, respectively. Crosshatching decreased the surface roughness values compared with unidirectional application (P = .003). Cell morphology observed in SEM showed that the elongation direction of cells resulted in all directions related with additional filopodia extentions within the crosshatch groups. Therefore, the highest cell viability was also detected in the three-directional crosshatch group (group 3) (P = .000) in XTT assay. According to the acridine orange test, higher cell numbers were seen in group 3, similar to the XTT findings (P = .86). Conclusion: Crosshatching significantly increased the cell-covered implant surfaces compared with the unidirectional group. Decreasing the surface Ra values via crosshatching helped spread the fibroblast over the implants in any direction, thus increasing cell proliferation and adhesion.
Açıklama
Anahtar Kelimeler
Electron Microscope Tomography, Lasers, Titanium
Kaynak
International Journal Of Oral & Maxillofacial Implants
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
32
Sayı
6
