The Effects of Laser with Different Parameters and Crosshatching on Fibroblast Adhesion and Proliferation to Implant Surfaces

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dc.contributor.authorCelebi, Hakki
dc.contributor.authorArpaci, Pembegul Uyar
dc.contributor.authorCelik, Ilhami
dc.contributor.authorAkman, Serhan
dc.date.accessioned2024-02-23T14:26:42Z
dc.date.available2024-02-23T14:26:42Z
dc.date.issued2017
dc.departmentNEÜen_US
dc.description.abstractPurpose: To compare the adhesion and proliferation effect of HGF-1 cells on pure titanium disks when the surfaces are unprocessed (machined surfaces after slicing) or modified with a laser. Materials and Methods: Twenty-eight titanium disks were divided into four groups. Three surface topographies were created using an erbium fiber laser: group 1 (unidirectional application [no crosshatch]), group 2 (crosshatching in two directions), and group 3 (crosshatching in three directions). The samples in group 4 were unprocessed and served as controls. The surface roughness of samples was investigated with scanning electron microscopy (SEM) and measured with a mechanical surface profilometer. HGF-1 cell line was used to analyze the adhesion of fibroblasts on the sample surfaces. The authors used SEM, XTT, and acridine orange tests to determine the adhesion of HGF-1 onto specimen surfaces and the cell morphology after incubation for 72 hours. XTT results and surface roughness values for all specimens were analyzed using the Kruskal-Wallis test. Results: Ra values of group 1 to group 4 (control) were 1.13, 0.26, 0.38, and 0.19, respectively. Crosshatching decreased the surface roughness values compared with unidirectional application (P = .003). Cell morphology observed in SEM showed that the elongation direction of cells resulted in all directions related with additional filopodia extentions within the crosshatch groups. Therefore, the highest cell viability was also detected in the three-directional crosshatch group (group 3) (P = .000) in XTT assay. According to the acridine orange test, higher cell numbers were seen in group 3, similar to the XTT findings (P = .86). Conclusion: Crosshatching significantly increased the cell-covered implant surfaces compared with the unidirectional group. Decreasing the surface Ra values via crosshatching helped spread the fibroblast over the implants in any direction, thus increasing cell proliferation and adhesion.en_US
dc.description.sponsorshipResearch Center of Selcuk University (BAP) Konya, Turkeyen_US
dc.description.sponsorshipThis work was supported in part by the Research Center of Selcuk University (BAP) Konya, Turkey. In addition, this study was a doctoral thesis. In 2014, the study was presented at a meeting of the European Prosthodontics Association in Istanbul, Turkey. The authors did not receive any financial support. The authors reported no conflicts of interest related to this study.en_US
dc.identifier.doi10.11607/jomi.5395
dc.identifier.endpage1240en_US
dc.identifier.issn0882-2786
dc.identifier.issn1942-4434
dc.identifier.issue6en_US
dc.identifier.pmid28518180en_US
dc.identifier.scopus2-s2.0-85034603792en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1231en_US
dc.identifier.urihttps://doi.org/10.11607/jomi.5395
dc.identifier.urihttps://hdl.handle.net/20.500.12452/14302
dc.identifier.volume32en_US
dc.identifier.wosWOS:000417113300006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherQuintessence Publishing Co Incen_US
dc.relation.ispartofInternational Journal Of Oral & Maxillofacial Implantsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectron Microscope Tomographyen_US
dc.subjectLasersen_US
dc.subjectTitaniumen_US
dc.titleThe Effects of Laser with Different Parameters and Crosshatching on Fibroblast Adhesion and Proliferation to Implant Surfacesen_US
dc.typeArticleen_US

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