Multipl skleroz hastalığı etiyopatogenezinde, İnsan Endojen Retrovirüs-W (HERV-W)'nın rolü
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Dosyalar
Tarih
2023
Yazarlar
Dergi Başlığı
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Cilt Başlığı
Yayıncı
Necmettin Erbakan Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Multipl skleroz (MS), merkezi sinir sisteminin nörodejeneratif bir hastalığıdır. Erken yaşta gelişir ve sıklıkla sekel oluşturur. Hastalığın etiyolojik nedeni tam olarak aydınlatılamamıştır ve henüz etkili bir tedavi mevcut değildir. Araştırmamız, İnsan endojen retrovirüslerinin ekspresyonu ile multipl sklerozun patogenezi arasındaki ilişkiyi ortaya koymayı amaçladık. Araştırmamızda Human Endogenous Retrovirus-W (HERV-W) env gen ekspresyon düzeyini belirlemeyi ve yine aynı gruplarda, HERV-W env gen ekspresyonunu sınırlama/düzenleme potansiyeli olan TRIM5 ve BST2 konak genlerinin ekspresyon düzeyini belirlemeyi hedefledik Bu çalışma Necmettin Erbakan Üniversitesi (Konya, Türkiye) Tıp Fakültesi Hastanesi Nöroloji Anabilim Dalı'nda McDonald (2017) tanı kriterlerine göre Relapsing-Remitting (RRMS) tanısı alan 50 takipli hasta ile nörodejeneratif hastalık dışlanmış yaş ve cinsiyet yönünden benzer 50 kişilik gönüllü kontrol olmak üzere 100 kişiyi dahil ettik. 50 RRMS tanısı almış hastaların yaşları 33.1 ortalamasındadır. Bu çalışma grubun hastalıklarının başlangıç yaşı 25,64 ortalaması, hastaların tanı alma yaş ortalaması 27,1 dir. Nöroloji kliniğinde rutin tanı, takip ve tedavi işlemleri devam eden hastalardan ve sağlıklı kan donörlerinden tam kan örnekleri kullanıldı. Gen ekspresyon düzeyleri, Real Time PCR yöntemi ile, CT değerleri ve referans gen CT değerleri kullanılarak fold-change analizi ile belirlendi. HERW-W ekspresyon düzeyleri için çalışma grupları arasında önemli bir farklılık saptanmadı. HERV-W gen ekspresyon düzeylerinin oldukça dinamik ve sofistike etkileşimlerden etkilenebileceği gerçeği, araştırmamızda gerçekleştirdiğimiz ölçümlerin birden fazla zamanlı ölçüm ve bir süreç analizi ile yapılmasının daha sağlıklı olacağı kanaati uyandırdı. BTS-2 gen ekspresyonu kontrol grubuna göre yaklaşık 3,5 kat artış göstermiştir. Sağlıklı kişiler ile hasta grubu arasında TRIM5α proteinin ekspresyonu açısından anlamlı bir fark tespit edilememiştir TRIM5 gen ekspresyon düzeyleri ile hastalık ve hastalığın seyrine yönelik anlamlı bir ilişki kurulamamıştır. BTS-2 gen ekspresyon düzeylerinin kontrol grubuna göre yüksek çıkması doğal bağışıklık yanıtın MS hastalığı üzerindeki etkisinin bir sonucu olabileceği yönünde değerlendirilmiştir.
Multiple sclerosis (MS) is a neurodegenerative disease of the central nervous system. It develops at an early age and often causes sequelae. The etiological cause of the disease has not been fully elucidated and there is no effective treatment yet. Our research aimed to reveal the relationship between the expression of human endogenous retroviruses and the pathogenesis of multiple sclerosis. In our study, we aimed to determine the Human Endogenous Retrovirus-W (HERV-W) env gene expression level and to determine the expression level of TRIM5 and BST2 host genes, which have the potential to limit/regulate HERV-W env gene expression. This study was conducted in Necmettin Erbakan University (Konya, Turkey) Medical Faculty Hospital Neurology Department with 50 follow-up patients diagnosed with Relapsing-Remitting (RRMS) according to McDonald (2017) diagnostic criteria, neurodegenerative disease excluded, and 50 volunteer controls who were similar in terms of age and gender. We included 100 people. The mean age of 50 patients diagnosed with RRMS is 33.1. The mean age of onset of the diseases in this study group was 25.64, and the mean age of diagnosis of the patients was 27.1. Whole blood samples with a volume of at least 2 ml, from healthy blood donors and patients whose routine diagnosis, follow-up and treatment procedures were carried out in the neurology clinic, were used. The expression level of genes was obtained for each sample by Real Time PCR, and was determined by fold-change analysis using CT values and reference gene CT values. No significant difference was found between the study groups for the HERW-W expression . The fact that HERV-W gene expression levels can be affected by highly dynamic and sophisticated interactions led to the conclusion that it would be healthier to make the measurements we performed in our research with more than one time measurement and a process analysis. Onthe other hand, we found that the expression of the BTS-2 gene increased approximately 3.5 times compared to the control group. However, no significant difference was found between the healthy subjects and the patient group in terms of expression of TRIM5α protein. A significant relationship could not be established between TRIM5 gene expression levels and the disease and its course. High levels of BTS-2 gene expression were evaluated as a result of the effect of innate immune response on MS disease.
Multiple sclerosis (MS) is a neurodegenerative disease of the central nervous system. It develops at an early age and often causes sequelae. The etiological cause of the disease has not been fully elucidated and there is no effective treatment yet. Our research aimed to reveal the relationship between the expression of human endogenous retroviruses and the pathogenesis of multiple sclerosis. In our study, we aimed to determine the Human Endogenous Retrovirus-W (HERV-W) env gene expression level and to determine the expression level of TRIM5 and BST2 host genes, which have the potential to limit/regulate HERV-W env gene expression. This study was conducted in Necmettin Erbakan University (Konya, Turkey) Medical Faculty Hospital Neurology Department with 50 follow-up patients diagnosed with Relapsing-Remitting (RRMS) according to McDonald (2017) diagnostic criteria, neurodegenerative disease excluded, and 50 volunteer controls who were similar in terms of age and gender. We included 100 people. The mean age of 50 patients diagnosed with RRMS is 33.1. The mean age of onset of the diseases in this study group was 25.64, and the mean age of diagnosis of the patients was 27.1. Whole blood samples with a volume of at least 2 ml, from healthy blood donors and patients whose routine diagnosis, follow-up and treatment procedures were carried out in the neurology clinic, were used. The expression level of genes was obtained for each sample by Real Time PCR, and was determined by fold-change analysis using CT values and reference gene CT values. No significant difference was found between the study groups for the HERW-W expression . The fact that HERV-W gene expression levels can be affected by highly dynamic and sophisticated interactions led to the conclusion that it would be healthier to make the measurements we performed in our research with more than one time measurement and a process analysis. Onthe other hand, we found that the expression of the BTS-2 gene increased approximately 3.5 times compared to the control group. However, no significant difference was found between the healthy subjects and the patient group in terms of expression of TRIM5α protein. A significant relationship could not be established between TRIM5 gene expression levels and the disease and its course. High levels of BTS-2 gene expression were evaluated as a result of the effect of innate immune response on MS disease.
Açıklama
Yüksek Lisans Tezi
Anahtar Kelimeler
BTS2, HERW-W, Multipl Skleroz (MS), TRİM5, BTS2, HERW-W, Multiple Sclerosis (MS), TRIM5
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Taşkın, Z. (2023). Multipl skleroz hastalığı etiyopatogenezinde, İnsan Endojen Retrovirüs-W (HERV-W)'nın rolü. (Yayımlanmamış yüksek lisans tezi). Necmettin Erbakan Üniversitesi, Sağlık Bilimleri Enstitüsü Tıbbi Mikrobiyoloji Anabilim Dalı, Konya.
