A rapid responsive coumarin-naphthalene derivative for the detection of cyanide ions in cell culture
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cyanide ion (CN-) is widely used in many industrial processes; however, it causes several diseases in humans. Therefore, rapid and accurate detection of CN- is very important and urgent. In this study, a CN- sensor (MH-2) which was capable of detecting CN- ions in living cell was developed. MH-2 gives a rapid color change, absorbance and fluorescence response to CN- in the presence of the anions tested in the working system. The binding ratio between the sensor and CN- was demonstrated by some spectrophotometric methods and the sensing mechanism was investigated by NMR titration experiments, suggesting that MH-2 gives response to CN- via the nucleophilic addition reaction. The fluorescence detection limit and the absorbance detection limit were calculated as 0.056 mu M and 0.11 mu M, respectively. Both of these detection limits are below the tolerable limit recommended by WHO for CN- in the drinking water (1.9 mu M). MH-2 was also applied to living cells for bio-imaging and the results showed that the sensor penetrates the cells and can detect cyanide ions in living cells.
Açıklama
Anahtar Kelimeler
Colorimetric Sensor, Fluorescent Sensor, Cyanide Sensing, Bioimaging
Kaynak
Analytical Biochemistry
WoS Q Değeri
Q2
Scopus Q Değeri
Q3
Cilt
654
