HPLC-DAD profiles and pharmacological insights of Onobrychis argyrea subsp isaurica extracts
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Onobrychis argyrea Boiss. subsp. Isaurica (Fabaceae), endemic to the eastern Mediterranean region, is a poorly studied medicinal plant. This study sets out to investigate into antioxidant and inhibitory activities of O. argyrea extracts (ethyl acetate, methanol, and water) against key enzymes linked to diabetes (alpha-amylase, alpha-glucosidase), Alzheimer's disease (acetylcholinesterase, butyrylcholinesterase), and skin hyperpigmentation (tyrosinase). Phytochemical composition was determined by HPLC-DAD and in silico approach used to provide additional insight of the possible interaction of the identified phenolic compounds with the studied enzymes. The methanol extract showed potent inhibitory action against acetylcholinesterase (1.55 mg GALAE/g extract), tyrosinase (61.61 mg KAE/g extract), and glucosidase (20.17 mmol ACAE/g extract). The methanol extract of O. argyrea exhibited potent radical scavenging potential (126.51 mg TE/g extract for DPPH scavenging assay) and reducing capacities (311.36 and 200.70 mg TE/g extract, for CUPRAC and FRAP assays, respectively). Quercetin, apigenin, and benzoic acid were identified in significant amounts in the methanol extract of O. argyrea. Quercetin interacted with the catalytic pocket of glucosidase by establishing hydrogen bonds with Ser157, Ser241, Asp307, and pi-pi interactions with His280 and Tyr158. The observed inhibitory effects of O. argyrea extracts on the studied enzyme suggest that this plant could be a promising source of naturally occurring chemical compounds for the management of diabetes, Alzheimer's disease, skin hyperpigmentation disorders, as well as, oxidative stress-related complications.
Açıklama
Anahtar Kelimeler
Onobrychis Argyrea, Hplc-Dad, Natural Agentsin Silico, Computational Approaches, Phyto-Pharmaceutics, Amylase, Glucosidase, Acetylcholinesterase
Kaynak
Computational Biology And Chemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
76
