HPLC-DAD profiles and pharmacological insights of Onobrychis argyrea subsp isaurica extracts

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dc.contributor.authorGuler, Gokalp Ozmen
dc.contributor.authorZengin, Gokhan
dc.contributor.authorKaradag, Firat
dc.contributor.authorMonica, Adriano
dc.contributor.authorPicot, Carene Marie Nancy
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.date.accessioned2024-02-23T14:02:36Z
dc.date.available2024-02-23T14:02:36Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractOnobrychis argyrea Boiss. subsp. Isaurica (Fabaceae), endemic to the eastern Mediterranean region, is a poorly studied medicinal plant. This study sets out to investigate into antioxidant and inhibitory activities of O. argyrea extracts (ethyl acetate, methanol, and water) against key enzymes linked to diabetes (alpha-amylase, alpha-glucosidase), Alzheimer's disease (acetylcholinesterase, butyrylcholinesterase), and skin hyperpigmentation (tyrosinase). Phytochemical composition was determined by HPLC-DAD and in silico approach used to provide additional insight of the possible interaction of the identified phenolic compounds with the studied enzymes. The methanol extract showed potent inhibitory action against acetylcholinesterase (1.55 mg GALAE/g extract), tyrosinase (61.61 mg KAE/g extract), and glucosidase (20.17 mmol ACAE/g extract). The methanol extract of O. argyrea exhibited potent radical scavenging potential (126.51 mg TE/g extract for DPPH scavenging assay) and reducing capacities (311.36 and 200.70 mg TE/g extract, for CUPRAC and FRAP assays, respectively). Quercetin, apigenin, and benzoic acid were identified in significant amounts in the methanol extract of O. argyrea. Quercetin interacted with the catalytic pocket of glucosidase by establishing hydrogen bonds with Ser157, Ser241, Asp307, and pi-pi interactions with His280 and Tyr158. The observed inhibitory effects of O. argyrea extracts on the studied enzyme suggest that this plant could be a promising source of naturally occurring chemical compounds for the management of diabetes, Alzheimer's disease, skin hyperpigmentation disorders, as well as, oxidative stress-related complications.en_US
dc.identifier.doi10.1016/j.compbiolchem.2018.07.016
dc.identifier.endpage263en_US
dc.identifier.issn1476-9271
dc.identifier.issn1476-928X
dc.identifier.pmid30081342en_US
dc.identifier.scopus2-s2.0-85050870592en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage256en_US
dc.identifier.urihttps://doi.org/10.1016/j.compbiolchem.2018.07.016
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11750
dc.identifier.volume76en_US
dc.identifier.wosWOS:000446282500027en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofComputational Biology And Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOnobrychis Argyreaen_US
dc.subjectHplc-Daden_US
dc.subjectNatural Agentsin Silicoen_US
dc.subjectComputational Approachesen_US
dc.subjectPhyto-Pharmaceuticsen_US
dc.subjectAmylaseen_US
dc.subjectGlucosidaseen_US
dc.subjectAcetylcholinesteraseen_US
dc.titleHPLC-DAD profiles and pharmacological insights of Onobrychis argyrea subsp isaurica extractsen_US
dc.typeArticleen_US

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