Psöriasis hastalarında total oksidan kapasite, total antioksidan kapasite, iskemik modifiye albumin, mikrorna seviyesi ve psöriasis alan şiddet indeksi ile ilişkisi
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada psöriasis olan ve olmayan bireylerde total antioksidan kapasite (TAS), total oksidan kapasite (TOS), iskemik modifiye albumin (İMA), mikro ribo nükleik asit (MikroRNA/miRNA/miR) düzeylerinin karşılaştırılması ve vaka grubunda bu değerlerin psöriasis alan şiddet indeksi (PASI) ile ilişkisinin araştırılması amaçlanmıştır. Gereç ve yöntem: Bu araştırma vaka-kontrol çalışması olarak tasarlandı. Vaka grubu olarak Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi Deri ve Zührevi Hastalıklar polikliniğine başvuran psöriasis tanısı almış, yeni ve takipli 91 hasta alındı. Kontrol grubu olarak Aile Hekimliği polikliniğine başvuran sistemik hastalığı olmayan, ilaç kullanmayan sağlıklı 91 birey alındı. Biyokimyasal parametre analizleri kit önerilerine uygun olarak Tıbbi Biyokimya Anabilim Dalı laboratuvarında aynı gün gerçekleştirildi. Veriler Statistical Package for the Social Sciences (SPSS) versiyon 20.0 istatistik paket programında değerlendirildi. İstatistiksel anlamlılık p<0.05 olarak kabul edildi. Bulgular: Vaka grubunda serum TOS seviyesi ortalaması 27,6±12,5 μmol H202 Equiv/L, kontrol grubunda 25,3±11,7 μmol H202 Equiv/L idi, ancak bu fark istatistiksel olarak anlamlı değil idi (p=0,206). Vaka grubunda ortalama serum TAS düzeyi ise anlamlı olarak daha düşük bulundu (p<0,001). Serum İMA ortalaması psöriasis mevcut olanlarda 24,3±16,8 ng/mL, olmayanlarda 40,8±25,8 ng/mL olarak tespit edildi. Psöriasis hastalarında ortalama serum İMA değerleri anlamlı derecede daha düşük idi (p<0,001). Vaka grubunda ortalama serum miR-203 ve miR-146a seviyesi artmış olarak tespit edildi. VKİ, TAS, TOS, İMA, miR-146a ile PASI ilişkili bulunmadı. miR-203 ile PASI arasında (r=0,232, p= 0,027) pozitif yönde zayıf güçte korelasyon, İMA ile TOS arasında (r=-0,235, p=0,025) negatif yönde zayıf korelasyon bulundu. TOS ile miR-146a arasında (r=0,257, p=0,014) pozitif yönde orta güçte korelasyon saptandı. Sonuç: Psöriasis hastalarında ortalama serum TOS düzeyi artmış, TAS düzeyi azalmış olarak bulundu. Bu oksidatif stresin hastalık patogenezinde kritik bir rol oynayabileceğine dair kanıt sunmaktadır. Vaka grubunda ortalama serum miR-203 ve miR-146a düzeyi artmış olarak bulunması nedeniyle bu miRNA'ları hedef alan antagonize miRNA terapileri yeni bir tedavi seçeneği olabilir. Böylece tedavi maliyetleri ve performans kaybı azalabilir, yaşam kalitesi artabilir. miR-203 ile PASI arasında zayıf korelasyon tespit edilmesi miR-203'ün hastalığın tedavi sonucunu değerlendirmekte bir biyobelirteç olarak kullanılabileceğini düşündürmektedir. Ancak tüm bu öneriler konusunda daha büyük ölçekli epidemiyolojik çalışmalara ihtiyaç vardır.
In this study, it was aimed to comparison of total antioxidant capacity (TAS), total oxidant capacity (TOS), ischemia-modified albumin (IMA), micro ribo nucleic acid (MicroRNA/miRNA/miR) levels in individuals with and without psoriasis and investigate the relationship between these values and psoriasis area severity index (PASI) in the case group. Materials and Methods: This research was designed as a case-control study. As a case group, 91 new and followed-up patients diagnosed with psoriasis who applied to Necmettin Erbakan University Meram Medical Faculty Skin and Venereal Diseases outpatient clinic were taken. As a control group, 91 healthy individuals without systemic disease and drug use, who applied to the Family Medicine outpatient clinic were taken. Biochemical parameter analyzes were performed on the same day in the Laboratory of Department of Medical Biochemistry in accordance with the kit recommendations. The data were evaluated in Statistical Package for the Social Sciences (SPSS) version 20.0 statistical package program. Statistical significance was accepted as p <0.05. Results: In the case group, the mean of the serum TOS level was 27.6 ± 12.5 μmol H202 Equiv / L, in the control group was 25.3 ± 11.7 μmol H202 Equiv / L, but this difference was not statistically significant (p=0,206). The mean of serum TAS level was significantly lower in the case group (p<0.001). Serum IMA mean value was 24.3±16.8 ng/mL in those with psoriasis and 40.8±25.8 ng/mL in those without psoriasis. The mean of serum IMA values were significantly lower in psoriasis patients (p<0.001). In the case group, mean serum miR-203 and miR-146a levels were detected to be increased. BMI, TAS, TOS, IMA, miR-146a and PASI were not associated. A positive weak correlation was found between miR-203 and PASI (r=0.232, p=0.027) and a negative weak correlation between IMA and TOS (r = -0.235, p=0.025). A positive moderate correlation was detected between TOS and miR-146a (r= 0.257, p=0.014). Conclusions: In patients with psoriasis, the mean serum TOS levels were increased and TAS levels were decreased. This provides evidence that oxidative stress can play a critical role in disease pathogenesis. Because of the increasing in the mean serum miR-203 and miR-146a levels in the case group, antagonizing therapies targeting these miRNAs may be a new treatment option. Thus, treatment costs and loss of performance may decrease and quality of life may increase. Since weak correlation between miR-203 and PASI was detected, disease activity and severity can be used as a biomarker to evaluate as a result of a therapeutic intervention. The weak correlation between miR-203 and PASI suggests that miR-203 can be used as a biomarker to evaluate the treatment outcome of the disease. However, larger-scale epidemiological studies are needed for all these recommendations.
In this study, it was aimed to comparison of total antioxidant capacity (TAS), total oxidant capacity (TOS), ischemia-modified albumin (IMA), micro ribo nucleic acid (MicroRNA/miRNA/miR) levels in individuals with and without psoriasis and investigate the relationship between these values and psoriasis area severity index (PASI) in the case group. Materials and Methods: This research was designed as a case-control study. As a case group, 91 new and followed-up patients diagnosed with psoriasis who applied to Necmettin Erbakan University Meram Medical Faculty Skin and Venereal Diseases outpatient clinic were taken. As a control group, 91 healthy individuals without systemic disease and drug use, who applied to the Family Medicine outpatient clinic were taken. Biochemical parameter analyzes were performed on the same day in the Laboratory of Department of Medical Biochemistry in accordance with the kit recommendations. The data were evaluated in Statistical Package for the Social Sciences (SPSS) version 20.0 statistical package program. Statistical significance was accepted as p <0.05. Results: In the case group, the mean of the serum TOS level was 27.6 ± 12.5 μmol H202 Equiv / L, in the control group was 25.3 ± 11.7 μmol H202 Equiv / L, but this difference was not statistically significant (p=0,206). The mean of serum TAS level was significantly lower in the case group (p<0.001). Serum IMA mean value was 24.3±16.8 ng/mL in those with psoriasis and 40.8±25.8 ng/mL in those without psoriasis. The mean of serum IMA values were significantly lower in psoriasis patients (p<0.001). In the case group, mean serum miR-203 and miR-146a levels were detected to be increased. BMI, TAS, TOS, IMA, miR-146a and PASI were not associated. A positive weak correlation was found between miR-203 and PASI (r=0.232, p=0.027) and a negative weak correlation between IMA and TOS (r = -0.235, p=0.025). A positive moderate correlation was detected between TOS and miR-146a (r= 0.257, p=0.014). Conclusions: In patients with psoriasis, the mean serum TOS levels were increased and TAS levels were decreased. This provides evidence that oxidative stress can play a critical role in disease pathogenesis. Because of the increasing in the mean serum miR-203 and miR-146a levels in the case group, antagonizing therapies targeting these miRNAs may be a new treatment option. Thus, treatment costs and loss of performance may decrease and quality of life may increase. Since weak correlation between miR-203 and PASI was detected, disease activity and severity can be used as a biomarker to evaluate as a result of a therapeutic intervention. The weak correlation between miR-203 and PASI suggests that miR-203 can be used as a biomarker to evaluate the treatment outcome of the disease. However, larger-scale epidemiological studies are needed for all these recommendations.
Açıklama
Anahtar Kelimeler
Psöriasis, PASI, TAS, TOS, İMA, miRNA, Psoriasis, PASI, TAS, TOS, IMA, miRNA
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Uzun, L. (2020). Psöriasis hastalarında total oksidan kapasite, total antioksidan kapasite, iskemik modifiye albumin, mikrorna seviyesi ve psöriasis alan şiddet indeksi ile ilişkisi. (Yayınlanmamış tıpta uzmanlık tezi) Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi Dahili Tıp Bilimleri Aile Hekimliği Anabilim Dalı, Konya.
