Celecoxib administration reduced mortality, mesenteric hypoperfusion, aortic dysfunction and multiple organ injury in septic rats
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier France-Editions Scientifiques Medicales Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: The cyclooxygenase (COX)-2 overexpression is associated with vascular injury and multiple organ failure in sepsis. However, constitutive COX-1 and basal COX-2 expressions have physiological effects. We aimed to investigate the effects of partial and selective COX-2 inhibition without affecting constitutive COX-1 and basal COX-2 activities by celecoxib on mesenteric artery blood flow (MABF), vascular reactivity, oxidative and inflammatory injuries, and survival in septic rats accomplished by cecal ligation and puncture (CLP). Methods: Wistar rats were allocated into Sham, CLP, Sham + celecoxib, CLP + celecoxib subgroups. 2 h after Sham and CLP operations, celecoxib (0.5 mg/kg) or vehicle (saline; 1 mL/kg) was administered orally to rats. 18 h after drug administrations, MABF and responses of isolated aortic rings to phenylephrine were measured. Tissue samples were obtained for biochemical and histopathological examinations. Furthermore, survival rate was monitored throughout 96 h. Results: Celecoxib ameliorated mesenteric hypoperfusion and partially improved aortic dysfunction induced by CLP. Survival rate was % 0 at 49th h in CLP group, but in CLP + celecoxib group it was 42.8% at the end of 96 h. Serum AST, ALT, LDH, BUN, Cr and inflammatory cytokine (tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6) levels were increased in CLP group that were prevented by celecoxib. The decreases in liver and spleen glutathione levels and the increases in liver, lung, spleen and kidney malondialdehyde levels in CLP group were blocked by celecoxib. The histopathological protective effects of celecoxib on organ injury due to CLP were also observed. Conclusions: Celecoxib has protective effects on sepsis due to its preservative effects on mesenteric perfusion, aortic function and its anti-inflammatory and antioxidative effects. (C) 2016 Elsevier Masson SAS. All rights reserved.
Açıklama
Anahtar Kelimeler
Celecoxib, Sepsis, Survival And Multiple Organ Damage, Vascular Hyporeactivity, Mesenteric Arterial Blood Flow
Kaynak
Biomedicine & Pharmacotherapy
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
86
