The effects of botulinum toxin A and papaverine on human saphenous vein and internal mammary artery grafts: an in vitro study
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Termedia Publishing House Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Introduction: Autologous saphenous vein (SV) and internal mammary ar-tery (IMA) are used as bypass conduits during coronary artery bypass graft surgery. Vasospasm of the arterial and venous grafts may constitute a sig-nificant clinical problem. Pretreatment with a vasodilator drug of the graft ex vivo or intraluminal injection before implantation may be used for spasm prophylaxis. This in vitro study was designed to assess the vasoactive ef-fects and time-dependent changes of botulinum toxin A (BTX-A) and pa-paverine pretreatment on vasospasm of human SV and IMA grafts. Also, histomorphology of the vessels was assessed.Material and methods: SV and IMA segments were suspended in organ baths, and isometric contraction responses to 2 different concentrations of 5-hydroxytryptamine (5-HT) and endothelin-1 (ET-1) were recorded after in-cubation with 2 different concentrations of BTX-A and papaverine at 2 time points (0 h and 2 h).Results: The results revealed the following: 1) incubation with BTX-A and papaverine relaxes both SV and IMA rings contracted with 5-HT and ET-1; 2) the duration of the relaxant effect of BTX-A lasts longer than papaverine; and 3) no apparent histomorphological changes were observed in the grafts under light microscopy.Conclusions: This study demonstrates that in human SV and IMA grafts, pretreatment with both BTX-A and papaverine are safe and have a potent inhibitory effect depending on the vessel and vasoconstrictor agent. The long-lasting vasodilatory effect of BTX-A on vascular smooth muscle may provide promising results in the prevention of venous and arterial graft spasm.
Açıklama
Anahtar Kelimeler
In Vitro, Papaverine, Botulinum Toxin A, Saphenous Vein, Human Internal Mammary Artery
Kaynak
Archives Of Medical Science
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
18
Sayı
6
