EFFECTS OF CURCUMIN TREATMENT ON CELL ENERGY STATUS, LEVELS OF MITOCHONDRIAL ENZYMES, AND GENE EXPRESSION OF GLUCOSE-RELATED MECHANISM IN PANCREATIC CANCER CELL LINES
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dokuz Eylul Univ Inst Health Sciences
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose: Curcumin is an active component of turmeric, has antitumor, immunomodulatory, anti-inflammatory effects. This study aimed to investigate the effects of the administration of curcumin on the energy metabolism, the abnormal redox defense mechanism profile, the malignant transformation indicator of Panc-1 and BxPC-3 pancreatic cancer cells.Material and Methods: BxPC-3 and Panc-1 cells were treated with various concentrations of curcumin (20 mu M, 40 mu M, 50 mu M, 60 mu M, 80 mu M, 100 mu M, and 125 mu M ) for 24 h. Cell lysate Adenosine triphosphate (ATP), Adenosine diphosphate (ADP), Adenosine monophosphate (AMP), Manganese superoxide dismutase (MnSOD), and cytochrome p450 reductase (CPR) concentrations were analyzed with HPLC and ELISA methods. Genes expression of Lactate dehydrogenase (LDH), mitochondrially encoded ATP synthase membrane subunit 6 (MTATP6), Glucose transporter 1 (GLUT1), and cytochrome p450 were analyzed by real-time quantitative PCR.Results: IC50 values for 24 hours were found as 47.26 mu M in BxPC-3 and 45.84 mu M in Panc-1 cells. Treatment with curcumin inhibited oxidative stress by increasing MnSOD enzyme levels. ATP levels did not change in BxPC-3 cells, but they increased in Panc-1 supplemented with curcumin. Gene expression of GLUT-1 was significantly down regulated when using at 45 mu M concentration of curcumin, which also affected glucose consumption in both cells. Conclusion: Curcumin showed anti-proliferative and antioxidant effects.
Açıklama
Anahtar Kelimeler
Atp, Bxpc-3, Curcumin, Cpr, Mnsod, Panc-1
Kaynak
Journal Of Basic And Clinical Health Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
7
Sayı
3
