EFFECTS OF CURCUMIN TREATMENT ON CELL ENERGY STATUS, LEVELS OF MITOCHONDRIAL ENZYMES, AND GENE EXPRESSION OF GLUCOSE-RELATED MECHANISM IN PANCREATIC CANCER CELL LINES

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dc.contributor.authorKorucu, Emine Nedime
dc.contributor.authorMenevse, Esma
dc.contributor.authorKaya, Dudu Erkoc
dc.contributor.authorGokturk, Fatma
dc.contributor.authorArikoglu, Hilal
dc.date.accessioned2024-02-23T14:34:27Z
dc.date.available2024-02-23T14:34:27Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractPurpose: Curcumin is an active component of turmeric, has antitumor, immunomodulatory, anti-inflammatory effects. This study aimed to investigate the effects of the administration of curcumin on the energy metabolism, the abnormal redox defense mechanism profile, the malignant transformation indicator of Panc-1 and BxPC-3 pancreatic cancer cells.Material and Methods: BxPC-3 and Panc-1 cells were treated with various concentrations of curcumin (20 mu M, 40 mu M, 50 mu M, 60 mu M, 80 mu M, 100 mu M, and 125 mu M ) for 24 h. Cell lysate Adenosine triphosphate (ATP), Adenosine diphosphate (ADP), Adenosine monophosphate (AMP), Manganese superoxide dismutase (MnSOD), and cytochrome p450 reductase (CPR) concentrations were analyzed with HPLC and ELISA methods. Genes expression of Lactate dehydrogenase (LDH), mitochondrially encoded ATP synthase membrane subunit 6 (MTATP6), Glucose transporter 1 (GLUT1), and cytochrome p450 were analyzed by real-time quantitative PCR.Results: IC50 values for 24 hours were found as 47.26 mu M in BxPC-3 and 45.84 mu M in Panc-1 cells. Treatment with curcumin inhibited oxidative stress by increasing MnSOD enzyme levels. ATP levels did not change in BxPC-3 cells, but they increased in Panc-1 supplemented with curcumin. Gene expression of GLUT-1 was significantly down regulated when using at 45 mu M concentration of curcumin, which also affected glucose consumption in both cells. Conclusion: Curcumin showed anti-proliferative and antioxidant effects.en_US
dc.description.sponsorshipNecmettin Erbakan University Scientific Research Coordinator [181215004]en_US
dc.description.sponsorshipThis work was supported by Necmettin Erbakan University Scientific Research Coordinator (project number:181215004) .en_US
dc.identifier.doi10.30621/jbachs.1180856
dc.identifier.endpage84en_US
dc.identifier.issn2458-8938
dc.identifier.issn2564-7288
dc.identifier.issue3en_US
dc.identifier.startpage70en_US
dc.identifier.urihttps://doi.org/10.30621/jbachs.1180856
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15621
dc.identifier.volume7en_US
dc.identifier.wosWOS:001099664700008en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherDokuz Eylul Univ Inst Health Sciencesen_US
dc.relation.ispartofJournal Of Basic And Clinical Health Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAtpen_US
dc.subjectBxpc-3en_US
dc.subjectCurcuminen_US
dc.subjectCpren_US
dc.subjectMnsoden_US
dc.subjectPanc-1en_US
dc.titleEFFECTS OF CURCUMIN TREATMENT ON CELL ENERGY STATUS, LEVELS OF MITOCHONDRIAL ENZYMES, AND GENE EXPRESSION OF GLUCOSE-RELATED MECHANISM IN PANCREATIC CANCER CELL LINESen_US
dc.typeArticleen_US

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