Effects of forskolin and rolipram on serum leptin, resistin and adiponectin levels in diet induced obesity in Wistar rats
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Dosyalar
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bir adenilat siklaz aktivatörü olan forskolin ve bir selektif fosfodiesteraz 4 inhibitörü olan rolipram siklik adenozin monofosfat (cAMP) seviyesini artırarak lipolizi stimule ve vücut ağırlık artışını inhibe ederler. Bu çalışmada forskolin ve rolipramın sıçanlarda diyetle indüklenen obezitede leptin, rezistin and adiponektin üzerine olan etkilerinin araştırılması amaçlanmıştır.Metod: 50 sıçan randomize olarak 5 gruba ayrıldı. Grup I standart pellet diyet ile diğer gruplar ise yüksek yağlı diyetle 10 hafta beslendi. Çalışmanın son iki haftasında grup II yüksek yağlı diyet ile beslenmeye devam ederken, grup III, grup IV ve grup V'e yüksek yağlı diyete ilave olarak sırası ile forskolin, rolipram ve forskolin ile rolipram orogastrik tüp ile verildi. Sonra sıçanlar sakrifiye edildi ve serum leptin, rezistin and adiponektin seviyeleri ölçüldü.Bulgular: Grup I ile kıyaslandığında Grup II (adiponektin için p0.001, leptin için p0.01), grup III (p0.01) ve grup V'in (p0.05) adiponektin seviyeleri anlamlı olarak azalmış, leptin seviyeleri ise anlamlı olarak artmıştı. Grup IV'ün leptin seviyesi grup II ile kıyaslandığında anlamlı olarak azalmıştı (p0.05). Grupların rezistin seviyeleri arasında anlamlı bir fark bulunamadı.Sonuç: Bulgularımız, rolipramın, cAMP seviyeleri üzerine olan etkisine ilave olarak diyetle indüklenen obezitede leptin ve adiponektin seviyelerinde herhangi bir değişiklik olmasını engellediğini göstermiştir. Ayrıca forskolin ve rolipram grupların rezistin seviyelerine etki göstermemiştir. Bu bulguları altında yatan mekanizmaların nedeni bilinmemekte ve daha sonraki çalışmalarda araştırılması gerekmektedir.
Objective: Forskolin, an activator of adenylate cyclase and rolipram a selective inhibitor of phosphodiesterase 4, stimulate lipolysis and inhibit body weight increase by increasing cyclic adenosine monophosphate (cAMP) levels. This study was aimed to investigate the effects of them on leptin, resistin and adiponectin levels in diet induced obesity in rats.Methods: Totally 50 rats were randomly divided into five groups. The Group I was fed with standard pellet diet and the other groups were fed with high-fat diet for 10 weeks. During the last two weeks of the study, group II continued to fed with high-fat diet whereas group III, group IV and group V were administered forskolin, rolipram and forskolin plus rolipram respectively by orogastric tube in addition to their high-fat diet. Then, rats were sacrificed and serum leptin, resistin and adiponectin levels were measured.Results: Although adiponectin levels of group II (p>0.001 for adiponectin, p>0.01 for leptin), group III (p>0.01), group V (p>0.05) were significantly decreased, leptin levels were significantly increased compared to that of the group I. Also, leptin levels of group IV were significantly reduced compared to those of group II (p>0.05). There were no significant differences between resistin levels of the groups. Conclusion: Our results showed that rolipram prevented any alteration in the levels of leptin and adiponectin in addition to its effect on cAMP levels. However, forskolin and rolipram showed no effect on resistin levels of the groups. The underlying mechanism of these findings is not known and needs to be more investigated.
Objective: Forskolin, an activator of adenylate cyclase and rolipram a selective inhibitor of phosphodiesterase 4, stimulate lipolysis and inhibit body weight increase by increasing cyclic adenosine monophosphate (cAMP) levels. This study was aimed to investigate the effects of them on leptin, resistin and adiponectin levels in diet induced obesity in rats.Methods: Totally 50 rats were randomly divided into five groups. The Group I was fed with standard pellet diet and the other groups were fed with high-fat diet for 10 weeks. During the last two weeks of the study, group II continued to fed with high-fat diet whereas group III, group IV and group V were administered forskolin, rolipram and forskolin plus rolipram respectively by orogastric tube in addition to their high-fat diet. Then, rats were sacrificed and serum leptin, resistin and adiponectin levels were measured.Results: Although adiponectin levels of group II (p>0.001 for adiponectin, p>0.01 for leptin), group III (p>0.01), group V (p>0.05) were significantly decreased, leptin levels were significantly increased compared to that of the group I. Also, leptin levels of group IV were significantly reduced compared to those of group II (p>0.05). There were no significant differences between resistin levels of the groups. Conclusion: Our results showed that rolipram prevented any alteration in the levels of leptin and adiponectin in addition to its effect on cAMP levels. However, forskolin and rolipram showed no effect on resistin levels of the groups. The underlying mechanism of these findings is not known and needs to be more investigated.
Açıklama
Anahtar Kelimeler
Biyokimya ve Moleküler Biyoloji, Forskolin, Rolipram, Leptin, Resistin, Adiponectin, Obesity, Wistar Rat, Rezistin, Adiponektin, Obezite, Wistar Sıçan
Kaynak
Türk Biyokimya Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
40
Sayı
3
Künye
Mehmetoğlu, İ., Döşeyici, S., Kurban, S., Taşyürek, E. (2015). Effects of forskolin and rolipram on serum leptin, resistin and adiponectin levels in diet induced obesity in Wistar rats. Türk Biyokimya Dergisi, 40, 3, 234-240.
