Pterjiyum etyopatogenezinde plasental büyüme faktörü, nöropilin-1 ve nöropilin-2 nin rolü
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Tarih
2022
Yazarlar
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Yayıncı
Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Pterjiyum etyopatogenezinde Plasental Büyüme Faktörü (PLGF), Nöropilin-1 (NP-1) ve Nöropilin-2’nin rolünü (NP-2) immunhistokimyasal olarak değerlendirmek.
Metot: Meram Tıp Fakültesi Göz Hastalıkları kliniğine başvuran ve daha önce pterjiyum cerrahisi geçirmiş olgular ile konjonktival nevüs eksizyonu yapılan olguların komşu temiz konjonktiva dokularından elde edilen preparatlar ve patolojik bloklar arşivden çıkarıldı. Tüm kesitlere, primer antikorlar olan 1/100 dilüe PLGF (abcam Cambridge Science Park, UK), %1/100 dilüe NP-1 ve NP-2 (abcam Cambridge Science Park, UK) immunhistokimyasal boyaları uygulandı. İmmunhistokimyasal boyama sonrası lamlar ışık mikroskobunda (Olympus BX53) değerlendirildi. Lam üzerindeki tüm pterjiyum doku alanı tarandı. Pterjiyum hücrelerinin epitel, endotel, stroma ve iltihap hücre elemanların boyanma şiddeti skorlaması ve ekspresyon yüzdesi değerlendirildi.
Bulgular: Çalışmaya dahil edilen retrospektif olarak incelenen 42 pterjiyum hastasının 19’u erkek, 23’ü kadındı. Kontrol grubu olarak çalışmaya dahil edilen 20 normal konjonktiva dokusunun 10’u erkek, 10’u kadın hastalardan elde edilmiştir. Gruplar arasında demografik özellikler açısından benzer sonuçlara ulaşılmıştır. Pterjiyum dokusunda epitel, endotel, stroma ve iltihap hücrelerindeki PLGF ve NP-2 düzeyleri normal konjonktiva dokusundaki aynı katmanlarla karşılaştırıldığında istatiksel olarak anlamlı derecede daha geniş alanda (p<0.001) ve yoğunluk olarak daha yüksek seviyede boyandığı görüldü (p<0.001). Ancak NP-1 ile boyanan pterjiyum ve normal konjonktiva dokusunun epitel, endotel, stroma ve iltihap hücreleri arasında hem yüzdesel alan olarak (p= 0.730, 0.121, 0.524, 0.624) hem de boyanma yoğunluğu olarak anlamlı bir fark saptanmadı (p= 0.716, 0.147, 0.147, 0.780).
Sonuç: Pterjiyum dokusunda yüksek düzeyde tespit edilen PLGF ve NP-2 pterjiyum progresyonu ve postoperatif nüksü önlemede gelecekte birer terapötik hedef olabilir.
To evaluate the role of Placental Growth Factor (PLGF), Neuropilin-1 (NP-1) and Neuropilin-2 (NP-2) in the etiopathogenesis of pterygium immunohistochemically. Method: Preparations and pathological blocks obtained from patients applied to Meram Medical Faculty Ophthalmology outpatient clinic who had previously undergone pterygium surgery and conjunctival nevus excision were removed from the archive. Immunohistochemical stains of the primary antibodies 1/100 diluted PLGF (abcam Cambridge Science Park, UK), 1/100% diluted NP-1 and NP-2 (abcam Cambridge Science Park, UK) were applied to all sections. After immunohistochemical staining, the slides were evaluated under an Olympus BX53 light microscope. The entire pterygium tissue area on the slide was scanned. Staining intensity scoring and expression percentage of epithelial, endothelial, stroma and inflammatory cell elements of pterygium cells were evaluated. Results: Of the 42 patients with pterygium included in the study, who were examined retrospectively, 19 were male and 23 were female. Of the 20 normal conjunctival tissues included in the study as the control group, 10 were taken from male and 10 from female patients. Similar results were obtained between the groups in terms of demographic characteristics. PLGF and NP-2 levels in epithelial, endothelial, stroma and inflammatory cells in pterygium tissue were statistically significantly larger (p<0.001) and higher in intensity when compared to the same layers in normal conjunctival tissue (p<0.001). However, no significant difference was found between the epithelium, endothelium, stroma and inflammatory cells of the pterygium and normal conjunctival tissue stained with NP-1, both in terms of percent area (p= 0.730, 0.121, 0.524, 0.624) and staining intensity. (p= 0.716, 0.147, 0.147, 0.780) Conclusion: High levels of PLGF and NP-2 detected in pterygium tissue may be therapeutic targets in the future to prevent pterygium progression and postoperative recurrence.
To evaluate the role of Placental Growth Factor (PLGF), Neuropilin-1 (NP-1) and Neuropilin-2 (NP-2) in the etiopathogenesis of pterygium immunohistochemically. Method: Preparations and pathological blocks obtained from patients applied to Meram Medical Faculty Ophthalmology outpatient clinic who had previously undergone pterygium surgery and conjunctival nevus excision were removed from the archive. Immunohistochemical stains of the primary antibodies 1/100 diluted PLGF (abcam Cambridge Science Park, UK), 1/100% diluted NP-1 and NP-2 (abcam Cambridge Science Park, UK) were applied to all sections. After immunohistochemical staining, the slides were evaluated under an Olympus BX53 light microscope. The entire pterygium tissue area on the slide was scanned. Staining intensity scoring and expression percentage of epithelial, endothelial, stroma and inflammatory cell elements of pterygium cells were evaluated. Results: Of the 42 patients with pterygium included in the study, who were examined retrospectively, 19 were male and 23 were female. Of the 20 normal conjunctival tissues included in the study as the control group, 10 were taken from male and 10 from female patients. Similar results were obtained between the groups in terms of demographic characteristics. PLGF and NP-2 levels in epithelial, endothelial, stroma and inflammatory cells in pterygium tissue were statistically significantly larger (p<0.001) and higher in intensity when compared to the same layers in normal conjunctival tissue (p<0.001). However, no significant difference was found between the epithelium, endothelium, stroma and inflammatory cells of the pterygium and normal conjunctival tissue stained with NP-1, both in terms of percent area (p= 0.730, 0.121, 0.524, 0.624) and staining intensity. (p= 0.716, 0.147, 0.147, 0.780) Conclusion: High levels of PLGF and NP-2 detected in pterygium tissue may be therapeutic targets in the future to prevent pterygium progression and postoperative recurrence.
Açıklama
Anahtar Kelimeler
Plasental Büyüme Faktörü, Nöropilin-1, Nöropilin-2, Vasküler Endotelyal Büyüme Faktörü, Pterjiyum, Kornea, Konjonktiva, Placental Growth Factor, Neuropilin-1, Neuropilin-2, Vascular Endothelial Growth Factor, Pterygium, Cornea, Conjunctiva
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Künye
Gündoğan, A. O. (2022). Pterjiyum etyopatogenezinde plasental büyüme faktörü, nöropilin-1 ve nöropilin-2 nin rolü. (Yayınlanmamış tıpta uzmanlık tezi) Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi Cerrahi Tıp Bilimleri Bölümü Göz Hastalıkları Anabilim Dalı, Konya.
