Combined effect of benzalkonium chloride and ultrasound against Listeria monocytogenes biofilm on plastic surface
Küçük Resim Yok
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study was performed to evaluate the effectiveness of benzalkonium chloride combined with ultrasound in eliminating Listeria monocytogenes biofilm from polystyrene surface. The test strain of L.monocytogenes, previously classified as strong biofilm producer, was grown to form biofilm in tryptic soy broth at 20 degrees C for 6days in polystyrene specimen containers. The biofilms formed on surface of containers were treated with ultrasound alone, benzalkonium chloride (100 and 400mgl(-1)) alone and their combination for three different exposure times (1, 5 and 15min) at room temperature. Sonication was performed using an ultrasound bath at a constant ultrasound frequency of 35kHz. After treatments, levels of biofilm biomass and viable cells in biofilm were determined using crystal violet staining and XTT assays, respectively. The combined treatment of ultrasound and benzalkonium chloride resulted in significant (P<005) more decrease in the level of viable cells in the L.monocytogenes biofilm compared to individual treatments of benzalkonium chloride. Our results suggest that the combination of benzalkonium chloride with ultrasound is useful approach for the elimination of L.monocytogenes biofilms from plastic surfaces.
Açıklama
Anahtar Kelimeler
Benzalkonium Chloride, Biofilm, Listeria Monocytogenes, Plastic Surface, Ultrasound
Kaynak
Letters In Applied Microbiology
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
57
Sayı
3
