Combined effect of benzalkonium chloride and ultrasound against Listeria monocytogenes biofilm on plastic surface

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dc.contributor.authorTorlak, E.
dc.contributor.authorSert, D.
dc.date.accessioned2024-02-23T14:24:32Z
dc.date.available2024-02-23T14:24:32Z
dc.date.issued2013
dc.departmentNEÜen_US
dc.description.abstractThis study was performed to evaluate the effectiveness of benzalkonium chloride combined with ultrasound in eliminating Listeria monocytogenes biofilm from polystyrene surface. The test strain of L.monocytogenes, previously classified as strong biofilm producer, was grown to form biofilm in tryptic soy broth at 20 degrees C for 6days in polystyrene specimen containers. The biofilms formed on surface of containers were treated with ultrasound alone, benzalkonium chloride (100 and 400mgl(-1)) alone and their combination for three different exposure times (1, 5 and 15min) at room temperature. Sonication was performed using an ultrasound bath at a constant ultrasound frequency of 35kHz. After treatments, levels of biofilm biomass and viable cells in biofilm were determined using crystal violet staining and XTT assays, respectively. The combined treatment of ultrasound and benzalkonium chloride resulted in significant (P<005) more decrease in the level of viable cells in the L.monocytogenes biofilm compared to individual treatments of benzalkonium chloride. Our results suggest that the combination of benzalkonium chloride with ultrasound is useful approach for the elimination of L.monocytogenes biofilms from plastic surfaces.en_US
dc.identifier.doi10.1111/lam.12100
dc.identifier.endpage226en_US
dc.identifier.issn0266-8254
dc.identifier.issn1472-765X
dc.identifier.issue3en_US
dc.identifier.pmid23682619en_US
dc.identifier.scopus2-s2.0-84881610653en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage220en_US
dc.identifier.urihttps://doi.org/10.1111/lam.12100
dc.identifier.urihttps://hdl.handle.net/20.500.12452/13994
dc.identifier.volume57en_US
dc.identifier.wosWOS:000322954700009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofLetters In Applied Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBenzalkonium Chlorideen_US
dc.subjectBiofilmen_US
dc.subjectListeria Monocytogenesen_US
dc.subjectPlastic Surfaceen_US
dc.subjectUltrasounden_US
dc.titleCombined effect of benzalkonium chloride and ultrasound against Listeria monocytogenes biofilm on plastic surfaceen_US
dc.typeArticleen_US

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