Çeşitli antiseptik taşıyıcılarla karıştırılan kalsiyum hidroksitin Enterococcus faecalis üzerindeki antimikrobiyal etkinliği
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Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu in vitro çalışmanın amacı farklı antiseptik taşıyıcı -
kalsiyum hidroksit (KH) kombinasyonlarının Enterococcus
faecalis (E. faecalis) ile enfekte kök kanallarındaki antibakteriyel
etkinliğini dentin blok modeli kullanarak test etmektir.
Gereç ve Yöntemler: Altmış adet çekilmiş insan dişi örnekleri
hazırlandı. Elli-beş tane kök segmenti E. faecalis ile 2 hafta
boyunca enfekte edildi. Örnekler 5 deney grubuna ayrıldı
(n=10) ve şu KH – antiseptik taşıyıcı karışımları uygulandı: Grup
1, KH + gliserin - distile su (7:1); Grup 2, KH + Octenisept;
Grup 3, KH + %2’lik klorheksidin glukonat (CHX); Grup 4, KH +
Savlex; Grup 5, KH + %5.25’lik sodyum hipoklorit (NaOCl).
Antiseptik-KH karışımları 7 gün boyunca kök kanallarında
bekletildi. Beş örnek sterilliği kontrol etmek için (negatif
kontrol), beş örnek de pozitif kontrol olarak kullanıldı. Gates
glidden frezler (# 3, 4, 5) yardımıyla kanal duvarlarından elde
edilen dentin örnekleri fosfat-tamponlu salin (PBS) ve cam
boncuk içeren şişelere aktarıldı ve 30 sn vortekslendi. Bakteri
içeren PBS’ye seri dilüsyon yapıldı. Her bir dilüsyondan 25 μL
halinde damlalar tripton soya agar plakları üzerine ekildi ve
37°C’de 48 saat inkübe edildi. Görünür koloni sayıları incelendi
ve log10 değerlerine dönüştürüldü. İstatistiksel analiz için
Kruskal-Wallis ve Mann Whitney-U testleri kullanıldı.
Bulgular: Negatif kontrol grubunda bakteri üremesi
görülmezken pozitif kontrol grubunun bütün örneklerinde
bakteri üremesi gözlemlenmiştir (log10 3,47 CFU ml-1
). Deney
grupları arasında istatistiksel olarak anlamlı bir fark
bulunmamıştır (p>0.05). KH + CHX ve KH + Savlex
gruplarında E. faecalis biyofilmi tamamen elimine edilmiştir.
Sonuç: Savlex, Octenisept ve NaOCl gibi çeşitli antiseptik
ajanlar KH ile karıştırılarak kullanıldığında kök kanallarından E.
faecalis’i elimine etmede etkili bulunmuştur.
Background: The aim of this in vitro study was to test disinfection ability of different new antiseptic vehicle-calcium hydroxide (CH) combinations in root canals infected with Enterococcus faecalis using a dentin block model. Methods: Sixty extracted human root samples were prepared. Fifty-five root segments were infected with E. faecalis for two weeks. Then, the samples were divided into 5 groups (n10) and following calcium hydroxide and antiseptic vehicle combinations were applied: Group 1, CH glycerin with distilled water (7:1); Group 2, CH Octenisept; Group 3, CH 2% chlorhexidine gluconate (CHX), Group 4, CH Savlex; Group 5, CH 5.25% sodium hypochlorite (NaOCl). Antiseptic-calcium hydroxide mixtures were applied for seven days. Five samples were used to check the sterility (negative control) and five samples were used as positive control. Dentin samples were obtained from the walls with gates-glidden burs (# 3, 4, 5) and transferred to vials with PBS/glass beads and vortexed for 30 sec. PBS with resuspended enterococci was then diluted. Droplets of 25 ?L from each dilution were inoculated on tryptic soy agar plates and incubated at 37C for 48 hour. Visible colonies from appropriated dilutions were counted and transformed to log10. Kruskal-Wallis and Mann Whitney-U test was used for the statistical analysis. Results: The negative control group remained free of growth, whereas the positive control group showed log10 CFU ml-1 of 3.47. No significant difference was observed among the test groups (p0.05). All E. faecalis cells were eliminated from the root canals in CH CHX and CH Savlex groups. Conclusion: The present study demonstrated the effectiveness of various antiseptic agents; Savlex, Octenisept, and NaOCl when used in mixing calcium hydroxide as vehicles in the elimination of E. faecalis from the root-canals.
Background: The aim of this in vitro study was to test disinfection ability of different new antiseptic vehicle-calcium hydroxide (CH) combinations in root canals infected with Enterococcus faecalis using a dentin block model. Methods: Sixty extracted human root samples were prepared. Fifty-five root segments were infected with E. faecalis for two weeks. Then, the samples were divided into 5 groups (n10) and following calcium hydroxide and antiseptic vehicle combinations were applied: Group 1, CH glycerin with distilled water (7:1); Group 2, CH Octenisept; Group 3, CH 2% chlorhexidine gluconate (CHX), Group 4, CH Savlex; Group 5, CH 5.25% sodium hypochlorite (NaOCl). Antiseptic-calcium hydroxide mixtures were applied for seven days. Five samples were used to check the sterility (negative control) and five samples were used as positive control. Dentin samples were obtained from the walls with gates-glidden burs (# 3, 4, 5) and transferred to vials with PBS/glass beads and vortexed for 30 sec. PBS with resuspended enterococci was then diluted. Droplets of 25 ?L from each dilution were inoculated on tryptic soy agar plates and incubated at 37C for 48 hour. Visible colonies from appropriated dilutions were counted and transformed to log10. Kruskal-Wallis and Mann Whitney-U test was used for the statistical analysis. Results: The negative control group remained free of growth, whereas the positive control group showed log10 CFU ml-1 of 3.47. No significant difference was observed among the test groups (p0.05). All E. faecalis cells were eliminated from the root canals in CH CHX and CH Savlex groups. Conclusion: The present study demonstrated the effectiveness of various antiseptic agents; Savlex, Octenisept, and NaOCl when used in mixing calcium hydroxide as vehicles in the elimination of E. faecalis from the root-canals.
Açıklama
Anahtar Kelimeler
Diş Hekimliği, Antimikrobiyal, E. faecalis, Kanal-içi medikament, Kök dentini, Antimicrobial, E. faecalis, Root canal medicament, Root dentin
Kaynak
Selcuk Dental Journal
WoS Q Değeri
Scopus Q Değeri
Cilt
4
Sayı
2
Künye
Akbulut, M. B., Güneşer, M. B., Ünverdi Eldeniz, A. (2017). Çeşitli antiseptik taşıyıcılarla karıştırılan kalsiyum hidroksitin Enterococcus faecalis üzerindeki antimikrobiyal etkinliği. Selcuk Dental Journal, 4, 2, 44-51.
