İmmünsupresif hastalarda cryptosporıdıum SPP. sıklığının çeşitli tanı yöntemleri ile araştırılması
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Tarih
2023
Yazarlar
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Yayıncı
Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada ishal şikayeti ile başvuran immünsupresif hastalarda Cryptosporidium spp. sıklığının mikroskobik, serolojik ve moleküler yöntemlerle araştırılması ve kullanılan yöntemlerin karşılaştırmalı olarak değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntem: İmmünsupresif ishalli hastalardan Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi Hastanesi Mikrobiyoloji Laboratuvarına gönderilen 90 dışkı örneği çalışmaya alınmıştır. Cryptosporidium spp. tespiti amacıyla dışkı örneklerine modifiye asit fast boyama, PZR, ELISA testi uygulanmıştır. Pozitif örneklerin de dahil olduğu 51 örnekte DFA yöntemiyle Cryptosporidium spp. araştırılmıştır. Bu yöntemlerden herhangi biriyle pozitif saptanan örneklerde ayrıca immünokromatografik kart test çalışılmıştır.
Bulgular: Çalışmaya alınan 90 örneğin 44 (%48,8)’ü erkek, 46 (%51,2)’sı kadın hastalara aittir. Hastaların yaşları 0 ile 81 yaş aralığında olup, yaş ortalaması 37,8 (standart sapma: ±27,5) olarak bulunmuştur. Çalışmada çocuk hastalara ait 31 (%34,4) numune bulunmaktadır ve bu hastaların yaş ortalamaları 5,3 (standart sapma: ±4,34) olarak tespit edilmiştir. Kullanılan yöntemlerin hiçbirinde hastaların pozitiflik durumu ile yaş arasında ve pozitiflik durumu ile cinsiyet arasında istatistiksel olarak anlamlı bir farklılık bulunmamıştır.
Çalışılan dışkı örneklerinde modifiye asit fast yöntemiyle 5 (%5,6), PZR ile 2 (%2,2), ELISA yöntemiyle 1 (%1,1) örnekte pozitiflik tespit edilmiştir. DFA yöntemi ile çalışılan örneklerin 6’sı pozitif bulunmuştur. Bu yöntemlerden herhangi biri ile pozitif bulunan 7 örnek immünokromatografik kart test ile araştırılmış ve 4 örnekte Cryptosporidium spp. pozitif saptanmıştır.
Sonuç: Çalışma immünsupresif ishalli hastalarda Cryptosporidium spp.’nin önemli bir etken olduğunu ortaya koymaktadır. DFA yöntemi altın standart yöntem olarak baz alındığında modifiye asit fast yöntemi yüksek duyarlılık ve özgüllükle, rutin tanıda kullanılabilecek bir yöntem olarak öne çıkmaktadır. Ayrıca yoğun iş akışı olan laboratuvarlarda immünokromatografik tanı testlerinin, pratik kullanımı ve hızlı sonuç alınması nedeniyle faydalı olacağı düşünülmüştür.
Objective: In this study, it was aimed to investigate the frequency of Cryptosporidium spp. in immunosuppressive patients with diarrhea by microscopic, serological and molecular methods and to evaluate the methods used comparatively. Materials and Methods: Ninety stool samples sent to Necmettin Erbakan University Meram Medical Faculty Hospital Microbiology Laboratory from immunosuppressive patients with diarrhea were included in the study. For the detection of Cryptosporidium spp., modified acid fast staining, PCR and ELISA test was applied to stool samples. Cryptosporidium spp was investigated by DFA method in 51 samples including positive samples. Immunochromatographic card test was also studied in cases that were found to be positive by any of these methods. Results: Of the 90 samples included in the study, 44 (48,8%) were male and 46 (51,2%) were female patients. The ages of the patients ranged from 0 to 81 years, with a mean age of 37,8 (standard deviation: ±27,5). There were 31 (34,4%) samples of pediatric patients in the study, and the mean age of these patients was 5.3 (standard deviation: ±4.34). No statistically significant difference was found between the positivity status of the patients and their age, and between the positivity status and gender in any of the methods used. In the stool samples studied, positivity was detected in 5 (5,6%) samples with the modified acid fast method, in 2 (2,2%) samples with PCR, and in 1 (1,1%) sample with the ELISA method. Positive results were found in 6 of the samples studied with the DFA method. 7 samples that were found positive by any of these methods were investigated with the immunochromatographic card test and Cryptosporidium spp. was positive in 4 samples. Conclusion: The study reveals that Cryptosporidium spp. is an important agent in patients with immunosuppressive diarrhea. Based on the DFA method as the gold standard method, the modified acid fast method stands out as a method that can be used in routine diagnosis with its high sensitivity and specificity.. In addition, it is thought that immunochromatographic diagnostic tests will be useful in laboratories with intensive workflow due to their practical use and rapid results.
Objective: In this study, it was aimed to investigate the frequency of Cryptosporidium spp. in immunosuppressive patients with diarrhea by microscopic, serological and molecular methods and to evaluate the methods used comparatively. Materials and Methods: Ninety stool samples sent to Necmettin Erbakan University Meram Medical Faculty Hospital Microbiology Laboratory from immunosuppressive patients with diarrhea were included in the study. For the detection of Cryptosporidium spp., modified acid fast staining, PCR and ELISA test was applied to stool samples. Cryptosporidium spp was investigated by DFA method in 51 samples including positive samples. Immunochromatographic card test was also studied in cases that were found to be positive by any of these methods. Results: Of the 90 samples included in the study, 44 (48,8%) were male and 46 (51,2%) were female patients. The ages of the patients ranged from 0 to 81 years, with a mean age of 37,8 (standard deviation: ±27,5). There were 31 (34,4%) samples of pediatric patients in the study, and the mean age of these patients was 5.3 (standard deviation: ±4.34). No statistically significant difference was found between the positivity status of the patients and their age, and between the positivity status and gender in any of the methods used. In the stool samples studied, positivity was detected in 5 (5,6%) samples with the modified acid fast method, in 2 (2,2%) samples with PCR, and in 1 (1,1%) sample with the ELISA method. Positive results were found in 6 of the samples studied with the DFA method. 7 samples that were found positive by any of these methods were investigated with the immunochromatographic card test and Cryptosporidium spp. was positive in 4 samples. Conclusion: The study reveals that Cryptosporidium spp. is an important agent in patients with immunosuppressive diarrhea. Based on the DFA method as the gold standard method, the modified acid fast method stands out as a method that can be used in routine diagnosis with its high sensitivity and specificity.. In addition, it is thought that immunochromatographic diagnostic tests will be useful in laboratories with intensive workflow due to their practical use and rapid results.
Açıklama
Anahtar Kelimeler
Cryptosporidium, İmmünsupresif, DFA, İshal, Cryptosporidium, Immunosuppressive, DFA, Diarrhea
Kaynak
WoS Q Değeri
Scopus Q Değeri
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Künye
Beder. D. (2023). İmmünsupresif hastalarda cryptosporıdıum SPP. sıklığının çeşitli tanı yöntemleri ile araştırılması. (Yayınlanmamış tıpta uzmanlık tezi) Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi Temel Tıp Bilimleri Bölümü Tıbbi Mikrobiyoloji Anabilim Dalı, Konya.
