Poli (laktik asit-KO-glikolik asit) (PLGA), gümüş ve tarçın emdirilmiş plga nanofiberlerin yara iyileşmesi üzerine etkisi: deneysel bir çalışma
Yükleniyor...
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Yara iyileşmesi tıbbın çözüm bekleyen konularından biridir. Yara iyileşmesini
hızlandırmak için geçmişten günümüze birçok ürün kullanılmıştır. Ancak ideal yara örtüsüne
ulaşma çabaları devam etmektedir. Mühendislik ve teknolojinin gelişmesi ile üretilen
nanomalzemeler, geniş temas yüzeyi sağlamaları ve birçok madde veya faktör ile kombine
edilmeleri nedeniyle yara iyileşmesi için ideal yara örtüsü çalışmalarında popüler hale
gelmektedirler. Bu çalışmada gümüş nanopartiküller ve tarçın özütü ile kombine edilen Poli
(Laktik Asit-Ko-Glikolik Asit) (PLGA) nanofiberlerin yara iyileşmesine etkisi incelenmiştir.
Yöntem: Bu çalışmada 24 adet sıçan her birinin sırt bölgesinde 2 cm² boyutunda ikişer adet
eksizyonel yara oluşturularak 6’şarlı gruplar halinde 4 gruba ayrılmıştır. Grup 1’de yara
pansuman yapılmadan takip edilirken, grup 2’de PLGA+AgNp+Tarçın (eAgNP/PLGA), grup
3’te saf PLGA, grup 4’te PLGA+AgNp (AgNP/PLGA) nanomalzemeler kullanılmıştır.
Yaralar 0., 3., 7., 14. ve 21. Günlerde fotoğraflanarak makroskobik açıdan değerlendirilmiştir.
7., 14. ve 21. Günlerde 6’şar adet yaradan biyopsiler alınarak Hematoksilen&Eozin(H&E)
boyama ile epitelizasyon, inflamasyon şiddeti ve tipi, CD-31 boyamada vaskülarizasyon, CD68 boyamada histiosit ve multinükleer dev hücre yoğunluğu ve MassonTrikrom (MTK) boyalı
lamlarda fibroblast proliferasyonu açısından değerlendirme yapılmıştır.
Bulgular: Makroskobik değerlendirmede 14. ve 21. günlerde grup 2 ve grup 4’te yara
iyileşme oranı anlamlı olarak grup 1 ve grup 3ten yüksek bulundu (p<0,05). 21. gün sonunda
grup 2’de üç adet, grup 4’te iki adet yara tam olarak kapandı. Histopatolojik incelemede Grup
2 ve Grup 4’ün grup 1 ve grup 3’e göre daha hızlı epitelize olduğu ve anjiogenezisin daha
fazla olduğu görüldü (p<0,05). 14.günde grup 3 inflamasyon şiddetinin grup 1’e göre anlamlı
olarak daha şiddetli olduğu saptandı (p<0,05). 21. Günde histiosit ve multinükleer dev hücre
yoğunluğu saf PLGA grubunda eAgNP/PLGA ve kontrol grubuna göre anlamlı olarak daha
yüksek bulundu (p<0,05).
Sonuç: Tarçın özütü ile indirgenmiş yeşil sentez metodu ile üretilen, gümüş nanopartiküllerin
katkılandığı PLGA nanofiberlerin anjiogenezisi artırarak epitelizasyonu hızlandırıp yara
iyileşmesini histopatolojik ve makroskobik açıdan olumlu etkileyeceğini düşünmekteyiz.
Objective: Wound healing is one of the outstanding issues of medicine. Many products have been used for years to accelerate wound healing. However, seeking for the ideal wound dressing has not ended. With the development of engineering and technology, nanomaterials have become more popular as wound dressing material that are used in wound healing studies, thanks to the fact that they provide large contact surfaces and are combined with many substances or factors. In this study, the effect of Poly (Lactic Acid-Co-Glycolic Acid) (PLGA) nanofibers, which were combined with silver nanoparticles and cinnamon extract, on wound healing was investigated. Method: In this study, 24 rats were divided into 4 groups, each compsed of 6 rats, by creating two excisional wounds in the size of 2 cm² on the dorsal region of each. While the wound was followed without dressing in group 1, PLGA+AgNp+Cinnamon (eAgNP/PLGA) nanomaterials were used in group 2, pure PLGA was used in group 3, and PLGA+AgNp (AgNP/PLGA) were used in group 4. Wounds were photographed and evaluated macroscopically on the days 0, 3, 7, 14 and 21. After skin biopsies were taken from each of 6 wounds on the 7th, 14th and 21st days; epithelialization and the severity and type of inflammation were evaluated from Hematoxylin&Eosin (H&E) stained specimens. Vascularization was evaluated from CD-31 stained specimens. Histiocyte and multinuclear giant cell density were evaluated from the CD-68 stained and fibroblast proliferation was evaluated from the Masson's Trichrome (MTK)stained slides. Result: In the macroscopic evaluation on the 14th and 21st days, the wound healing rate in group 2 and group 4 was found to be significantly higher than group in 1 and 3 (p<0.05). At the end of the 21st day, three wounds in group 2 and two wounds in group 4 were completely healed. In the histopathological examination, it was observed that the wounds in Group 2 and Group 4 epithelialized faster than the wounds in group 1 and group 3 and angiogenesis was higher, as well (p<0.05). On the 14th day, the severity of inflammation in group 3 was found to be significantly more severe than group 1 (p<0.05). On day 21, histiocyte and multinuclear giant cell density were found to be significantly higher in the pure PLGA group than in the eAgNP/PLGA and control groups (p<0.05). Conclusion: We think that the PLGA nanofibers which were produced by using the green synthesis method that reduced by cinnamon extract and were enriched with silver nanoparticles will increase angiogenesis, accelerate epithelialization and positively affect wound healing histopathologically and macroscopically.
Objective: Wound healing is one of the outstanding issues of medicine. Many products have been used for years to accelerate wound healing. However, seeking for the ideal wound dressing has not ended. With the development of engineering and technology, nanomaterials have become more popular as wound dressing material that are used in wound healing studies, thanks to the fact that they provide large contact surfaces and are combined with many substances or factors. In this study, the effect of Poly (Lactic Acid-Co-Glycolic Acid) (PLGA) nanofibers, which were combined with silver nanoparticles and cinnamon extract, on wound healing was investigated. Method: In this study, 24 rats were divided into 4 groups, each compsed of 6 rats, by creating two excisional wounds in the size of 2 cm² on the dorsal region of each. While the wound was followed without dressing in group 1, PLGA+AgNp+Cinnamon (eAgNP/PLGA) nanomaterials were used in group 2, pure PLGA was used in group 3, and PLGA+AgNp (AgNP/PLGA) were used in group 4. Wounds were photographed and evaluated macroscopically on the days 0, 3, 7, 14 and 21. After skin biopsies were taken from each of 6 wounds on the 7th, 14th and 21st days; epithelialization and the severity and type of inflammation were evaluated from Hematoxylin&Eosin (H&E) stained specimens. Vascularization was evaluated from CD-31 stained specimens. Histiocyte and multinuclear giant cell density were evaluated from the CD-68 stained and fibroblast proliferation was evaluated from the Masson's Trichrome (MTK)stained slides. Result: In the macroscopic evaluation on the 14th and 21st days, the wound healing rate in group 2 and group 4 was found to be significantly higher than group in 1 and 3 (p<0.05). At the end of the 21st day, three wounds in group 2 and two wounds in group 4 were completely healed. In the histopathological examination, it was observed that the wounds in Group 2 and Group 4 epithelialized faster than the wounds in group 1 and group 3 and angiogenesis was higher, as well (p<0.05). On the 14th day, the severity of inflammation in group 3 was found to be significantly more severe than group 1 (p<0.05). On day 21, histiocyte and multinuclear giant cell density were found to be significantly higher in the pure PLGA group than in the eAgNP/PLGA and control groups (p<0.05). Conclusion: We think that the PLGA nanofibers which were produced by using the green synthesis method that reduced by cinnamon extract and were enriched with silver nanoparticles will increase angiogenesis, accelerate epithelialization and positively affect wound healing histopathologically and macroscopically.
Açıklama
Anahtar Kelimeler
Enkapsüle Gümüş Nanopartikül, PLGA Nanofiber, Tarçın, Yara İyileşmesi, Cinnamon, Encapsulated silver nanoparticles, PLGA nanofibers, Wound healing
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Baycar. Z. (2022). Poli (laktik asit-KO-glikolik asit) (PLGA), gümüş ve tarçın emdirilmiş plga nanofiberlerin yara iyileşmesi üzerine etkisi: deneysel bir çalışma. (Yayınlanmamış tıpta uzmanlık tezi) Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi Cerrahi Tıp Bilimleri Bölümü Plastik Cerrahi Anabilim Dalı, Konya.
