Lack of Association of 1513 A/C Polymorphism in P2X7 Gene with Susceptibility to Pulmonary and Extrapulmonary Tuberculosis
Yükleniyor...
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Giriş: Latent, pulmoner ve ekstrapulmoner şeklinde farklı klinik formları olan tüberküloz, dünya genelinde ölüme sebebiyet veren
hastalıkların en önemlilerinden biridir. Aday genlerle yapılan çalışmalarda yaygın polimorfizmlerin tüberkülozun gelişmesinde etkili
olabileceği sonucuna varılmıştır. Bu çalışmada P2X7 genindeki 1513 A/C polimorfizminin tüberkülozun etyopatogenezisindeki rolünün ortaya çıkarılması amaçlanmıştır.
Materyal ve Metod: Bu çalışma 160 tüberkülozlu (71 pulmoner tüberkülozlu ve 89 ekstrapulmoner tüberkülozlu) 160 da sağlıklı
bireyi kapsamaktadır. Genomik DNA izolasyonu gerçekleştirildikten sonra P2X7 genindeki 1513 A/C polimorfizmi PCR-RFLP metoduyla genotiplendirildi.
Bulgular: AA genotipinin frekansı kontrol grubunda %47.5, hastalarda %56.87, AC genotipinin frekansı kontrol grubunda %39.37,
hastalarda %32.5, CC genotipinin frekansı kontrol grubunda %13.12, hastalarda %10.62 olarak saptandı. Hastalar ile kontrol grubunun allel ve genotip frekansları arasında anlamlı bir farklılık bulunamadı.
Sonuç: Sonuçlarımız Türkiye’nin doğu bölgesinde P2X7 genindeki, 1513 A/C polimorfizminin pulmoner ve ekstrapulmoner tüberküloz hastalığıyla ilişkisinin olmadığını gösterdi.
Introduction: Tuberculosis, is one of the a leading causes of death worldwide, is characterized by different clinical forms including: latent, localized pulmonary infection and extrapulmonary tuberculosis. Candidate gene association studies have implicated common polymophisms in genes that may influence the development of tuberculosis. This study, aimed to elucidate the role of P2X7 gene in 7 1513A/C polymorphism the etiopathogenesis of tuberculosis. Materials and Methods: The study included 160 patients with tuberculosis (71 pulmonary and 89 extrapulmonary tuberculosis) and 160 healthy controls. Genomic DNA was isolated and 1513A/C polymorphism in P2X7 gene was genotyped by PCR-RFLP method. Results: Frequency of P2X7 AA genotype was 47.5% in controls and 56.87% in patients, AC frequency was 39.37% controls and 32.5% in patients, CC genotype was 13.12% in controls and 10.62% in patients. No significant difference in allele and genotype frequencies (1513A/C polymorphism) between tuberculosis patients and controls was found. Conclusion: The results suggest that 1513A/C polymorphism of P2X7 gene is not associated with pulmonary and extrapulmonary tuberculosis in the Eastern Turkey.
Introduction: Tuberculosis, is one of the a leading causes of death worldwide, is characterized by different clinical forms including: latent, localized pulmonary infection and extrapulmonary tuberculosis. Candidate gene association studies have implicated common polymophisms in genes that may influence the development of tuberculosis. This study, aimed to elucidate the role of P2X7 gene in 7 1513A/C polymorphism the etiopathogenesis of tuberculosis. Materials and Methods: The study included 160 patients with tuberculosis (71 pulmonary and 89 extrapulmonary tuberculosis) and 160 healthy controls. Genomic DNA was isolated and 1513A/C polymorphism in P2X7 gene was genotyped by PCR-RFLP method. Results: Frequency of P2X7 AA genotype was 47.5% in controls and 56.87% in patients, AC frequency was 39.37% controls and 32.5% in patients, CC genotype was 13.12% in controls and 10.62% in patients. No significant difference in allele and genotype frequencies (1513A/C polymorphism) between tuberculosis patients and controls was found. Conclusion: The results suggest that 1513A/C polymorphism of P2X7 gene is not associated with pulmonary and extrapulmonary tuberculosis in the Eastern Turkey.
Açıklama
Anahtar Kelimeler
Tüberküloz, P2X7, Polimorfizm, Tuberculosis, Polymorphism
Kaynak
Tüberküloz ve Toraks
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
62
Sayı
1
Künye
Özdemir, F. A., Erol, D., Konar, V., Yüce, H., Karaşenli, E., Bulut, F., Deveci, F. (2014). Lack of association of 1513 a/c polymorp hism in p2x, gene with susceptibility to pulmonary and extrap ulmonary tubercu losisul. Tüberküloz ve Toraks, 62, 1, 7-11.
