Determination of carcinoembryonic antigen (CEA) by surface plasmon resonance-enhanced total internal reflection ellipsometry (SPRe-TIRE)
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
A surface plasmon resonance-enhanced total internal reflection ellipsometric (SPRe-TIRE) aptasensor is reported for the determination of the carcinoembryonic antigen (CEA). A gold nanofilm-coated SF10 glass slide was modified with electrochemical diazonium salt reduction and gold nanoparticles (AuNPs) to form a sensor. Next, a specific aptamer for CEA was immobilized on the AuNPs via self-assembly. The platform was characterized by X-ray photoelectron spectroscopy, ellipsometry, and cyclic voltammetry for each step of the preparation of the sensor. CEA aptamers with different affinities were used. SPRe-TIRE employed total internal reflection geometry and AuNPs to enhance plasmon resonance for the sensitive and selective determination of CEA in human serum. The working range of the aptasensor was from 0.01 to 500 ng/mL with an optimum detection limit of 0.1 pg/mL.
Açıklama
Anahtar Kelimeler
Carcinoembryonic Antigen (Cea), Surface Plasmon Resonance-Enhanced Total Internal Reflection Ellipsometry (Spre-Tire), Aptasensor, Cyclic Voltammetry (Cv)
Kaynak
Instrumentation Science & Technology
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
52
Sayı
2
