In vitro study of the toxic and teratogenic effects of prednisolone, azathioprine and mycophenolate mofetile on embryological development of rats
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study aimed to evaluate the effects of the glucocorticoid prednisolone, the mycophenolic acid prodrug, azathioprine, and the fungi fermentation end product, mycophenolate mofetile on the embryological development of rats. Nine day-old rat embryos were cultured in rat serum containing prednisolone at varying concentrations (5-30 mu g/ml) for 48 h. The test groups were cultured separately in rat serum containing 0.3-10 mu g/ml azathioprine and 1-10 mu g/ml mycophenolate mofetile. Embryonic development parameter effects of both drugs in combination with prednisolone (20 mu g/ml) were studied using morphological methods, with special attention given to the incidence of malformations. The genotoxic effects of agents evaluated with the TUNEL test revealed that prednisolone is not a cause of developmental toxicity. The maximum safe dose of prednisolone that could be used in combination with other immunosuppressive agents was determined to be 20 mu g/ml. Azathioprine was found to be toxic and teratogenic for the rat embryos beginning at a dose of 1 mu g/ml. Dose-dependent toxic and teratogenic effects of mycophenolate mofetile were detected at doses lower than normal clinical ones.
Açıklama
Anahtar Kelimeler
Azathioprine, Mycophenolate Mofetile, Prednisolone, Tunel Assay, Whole Embryo Culture
Kaynak
Drug And Chemical Toxicology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
45
Sayı
6
