In vitro study of the toxic and teratogenic effects of prednisolone, azathioprine and mycophenolate mofetile on embryological development of rats

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dc.contributor.authorFazliogullari, Zeliha
dc.contributor.authorKarabulut, Ahmet Kagan
dc.contributor.authorUnver Dogan, Nadire
dc.contributor.authorUysal, Ismihan Ilknur
dc.contributor.authorAcar, Hasan
dc.date.accessioned2024-02-23T14:17:13Z
dc.date.available2024-02-23T14:17:13Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractThis study aimed to evaluate the effects of the glucocorticoid prednisolone, the mycophenolic acid prodrug, azathioprine, and the fungi fermentation end product, mycophenolate mofetile on the embryological development of rats. Nine day-old rat embryos were cultured in rat serum containing prednisolone at varying concentrations (5-30 mu g/ml) for 48 h. The test groups were cultured separately in rat serum containing 0.3-10 mu g/ml azathioprine and 1-10 mu g/ml mycophenolate mofetile. Embryonic development parameter effects of both drugs in combination with prednisolone (20 mu g/ml) were studied using morphological methods, with special attention given to the incidence of malformations. The genotoxic effects of agents evaluated with the TUNEL test revealed that prednisolone is not a cause of developmental toxicity. The maximum safe dose of prednisolone that could be used in combination with other immunosuppressive agents was determined to be 20 mu g/ml. Azathioprine was found to be toxic and teratogenic for the rat embryos beginning at a dose of 1 mu g/ml. Dose-dependent toxic and teratogenic effects of mycophenolate mofetile were detected at doses lower than normal clinical ones.en_US
dc.description.sponsorshipScientific Research Project Commission of Selcuk University, Konya, Turkey [10401118]en_US
dc.description.sponsorshipThis project was supported by Scientific Research Project Commission of Selcuk University, Konya, Turkey [Grant no. 10401118].en_US
dc.identifier.doi10.1080/01480545.2021.1985926
dc.identifier.endpage2747en_US
dc.identifier.issn0148-0545
dc.identifier.issn1525-6014
dc.identifier.issue6en_US
dc.identifier.pmid34719311en_US
dc.identifier.scopus2-s2.0-85118445533en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2739en_US
dc.identifier.urihttps://doi.org/10.1080/01480545.2021.1985926
dc.identifier.urihttps://hdl.handle.net/20.500.12452/13002
dc.identifier.volume45en_US
dc.identifier.wosWOS:000713499800001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofDrug And Chemical Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAzathioprineen_US
dc.subjectMycophenolate Mofetileen_US
dc.subjectPrednisoloneen_US
dc.subjectTunel Assayen_US
dc.subjectWhole Embryo Cultureen_US
dc.titleIn vitro study of the toxic and teratogenic effects of prednisolone, azathioprine and mycophenolate mofetile on embryological development of ratsen_US
dc.typeArticleen_US

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