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    The Adiponectin variants contribute to the genetic background of type 2 diabetes in Turkish population
    (Elsevier, 2014) Arikoglu, Hilal; Ozdemir, Hulya; Kaya, Dudu Erkoc; Ipekci, Suleyman Hilmi; Arslan, Ahmet; Kayis, Seyit Ali; Gonen, Mustafa Sait
    Adiponectin, an adipose tissue specific protein encoded by the Adiponectin gene, modulates insulin sensitivity and plays an important role in regulating energy homeostasis. Many studies have shown that single nucleotide polymorphisms (SNPs) in the Adiponectin gene are associated with low plasma Adiponectin levels, insulin resistance and an increased risk of type 2 diabetes mellitus. The aim of the present study was to evaluate the contribution of the Adiponectin gene polymorphisms in genetic background of type 2 diabetes in a Turkish population. In total, 169 unrelated and non-obese diabetic patients and 119 age- and BMI-matched nondiabetic individuals with no family history of diabetes were enrolled in this study. We detected a significant association between type 2 diabetes and two SNPs: SNP - 11391G>A. which is located in the promoter region of the Adiponectin gene, and SNP + 276G > T, which is found in intron 2 of the gene (P < 0.05). The silence SNP G15G ( + 45T > G) in exon 1 and SNP + 349A > G in intron 2 also showed a weak association with type 2 diabetes (P = 0.06 and P = 0.07, respectively), while SNPs - 3971A>G in intron 1 and Y111H, R112C and H241P in exon 3 showed no association (P > 0.05). In conclusion, these findings suggest that Adiponectin gene polymorphisms might be effective on susceptibility for type 2 diabetes development which emerged from the interactions between multiple genes, variants and environmental factors. (C) 2013 Elsevier B.V. All rights reserved.
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    EFFECTS OF CURCUMIN TREATMENT ON CELL ENERGY STATUS, LEVELS OF MITOCHONDRIAL ENZYMES, AND GENE EXPRESSION OF GLUCOSE-RELATED MECHANISM IN PANCREATIC CANCER CELL LINES
    (Dokuz Eylul Univ Inst Health Sciences, 2023) Korucu, Emine Nedime; Menevse, Esma; Kaya, Dudu Erkoc; Gokturk, Fatma; Arikoglu, Hilal
    Purpose: Curcumin is an active component of turmeric, has antitumor, immunomodulatory, anti-inflammatory effects. This study aimed to investigate the effects of the administration of curcumin on the energy metabolism, the abnormal redox defense mechanism profile, the malignant transformation indicator of Panc-1 and BxPC-3 pancreatic cancer cells.Material and Methods: BxPC-3 and Panc-1 cells were treated with various concentrations of curcumin (20 mu M, 40 mu M, 50 mu M, 60 mu M, 80 mu M, 100 mu M, and 125 mu M ) for 24 h. Cell lysate Adenosine triphosphate (ATP), Adenosine diphosphate (ADP), Adenosine monophosphate (AMP), Manganese superoxide dismutase (MnSOD), and cytochrome p450 reductase (CPR) concentrations were analyzed with HPLC and ELISA methods. Genes expression of Lactate dehydrogenase (LDH), mitochondrially encoded ATP synthase membrane subunit 6 (MTATP6), Glucose transporter 1 (GLUT1), and cytochrome p450 were analyzed by real-time quantitative PCR.Results: IC50 values for 24 hours were found as 47.26 mu M in BxPC-3 and 45.84 mu M in Panc-1 cells. Treatment with curcumin inhibited oxidative stress by increasing MnSOD enzyme levels. ATP levels did not change in BxPC-3 cells, but they increased in Panc-1 supplemented with curcumin. Gene expression of GLUT-1 was significantly down regulated when using at 45 mu M concentration of curcumin, which also affected glucose consumption in both cells. Conclusion: Curcumin showed anti-proliferative and antioxidant effects.
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    The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines
    (Makerere Univ, Coll Health Sciences,Sch Med, 2022) Arikoglu, Hilal; Dursunoglu, Duygu; Kaya, Dudu Erkoc; Avci, Ebru
    Background: Pancreatic cancer does not show any symptoms in the early period and metastatic process is already passed when the diagnosis is done. Therefore, in the battle with pancreatic cancer, novel treatment strategies, particularly antiinva-sive and antimetastatic strategies, are needed. The cytotoxic and anticancer effects of juglone and sodium selenite (NaSe) have been showed in various cancer cells. Objectives: In this study, it is aimed to investigate the synergistic effects of juglone and selenium on PANC-1 and BxPC-3 pancreatic cancer cells. Methods: Antimetastatic effects of juglone-NaSe were carried out by adhesion and invasion assays and the genes and pro-tein expressions. Expression analysis of the CDH1, ITGB3 and COL4A3 genes and their proteins E-cadherin, beta 3 integrin and tumstatin which play role in metastasis and angiogenesis processes, were done by qPCR and immunohistochemical analysis, respectively. Results: Study findings have provided evidences that the juglone-selenium has a cytotoxic and dose dependent suppressive effect on invasion and metastasis in PANC-1 and BxPC-3 cells. Conclusion: The juglone-NaSe has the potential to be a promising agent especially to inhibit invasion and metastasis in pan-creatic cancer treatment. However, more in depth studies are needed to more clearly demonstrate the effects of juglone-se-lenium.
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    Investigation of juglone effects on metastasis and angiogenesis in pancreatic cancer cells
    (Elsevier Science Bv, 2016) Avci, Ebru; Arikoglu, Hilal; Kaya, Dudu Erkoc
    Juglone, a natural component, is shown to have cytotoxic and apoptotic effects in several cancer cell lines. However, little is known about its effects on invasion and metastasis. In this study, we aimed to determine the antimetastatic effect of juglone in the BxPC-3 and PANC-1 pancreatic cancer cell lines. Cytotoxic effect of juglone was evaluated by using MTT (3-(4,5-Dimethylthiazo1-2-yl)-2,5-Diphenyltetrazolium Bromide) test. The cells were treated with juglone at