Yazar "Ceylan, Ramazan" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • Küçük Resim Yok
    Öğe
    Bioactive constituents of Lathyrus czeczottianus and ethyl acetate and water extracts and their biological activities: An endemic plant to Turkey
    (Elsevier, 2021) Ceylan, Ramazan; Zengin, Gokhan; Guler, Gokalp Ozmen; Aktumsek, Abdurrahman
    Lathyrus species are economically important as food, fodder, and folk medicine. The antioxidant properties (radical scavenging effectiveness, reducing power potentials, metal chelating ability, phosphomolydenum capacity) phytochemical characterization (phenolic composition), and enzyme inhibition abilities of the water and ethyl acetate extracts of Lathyrus czeczottianus (LC) were tested. The phenolic ingradients of LC extracts were evaluated by HPLC-DAD (High-performance liquid chromatography with diode array detector). Chlorogenic acid, epicatechin, and benzoic acid were found as main phenolic compounds in both extracts. Generally, the water extract revealed strong antioxidant potential. The antioxidant effectiveness could be explained by high total phenolic level in the extract. Regarding enzyme inhibitory results, the ethyl acetate extract indicated good inhibitory activities on cholinesterase (for AChE: Acetylccholinesterase 1.15 mg galathamine equivalents (GALAE/g extract) and for BChE: Butyrylcholinesterase 0.19 mg GALAE/g extrac), tyrosinase (54.48 mg kojic acid equivalents (KAE/g)), and alpha-amylase (0.34 mmol acarbose equivalents (ACEs/g)), while the water extract had just good inhibition on alpha-glucosidase (6.49 mmol ACEs/g). In conclusion, L. czeczottianus could be good valuable of bioactive molecules for pharmaceuticals, food and agriculture, and nutraceutical applications. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.
  • Küçük Resim Yok
    Öğe
    Chemical and biological fingerprints of two Fabaceae species (Cytisopsis dorycniifolia and Ebenus hirsuta): Are they novel sources of natural agents for pharmaceutical and food formulations?
    (Elsevier Science Bv, 2016) Ceylan, Ramazan; Katanic, Jelena; Zengin, Gokhan; Matic, Sanja; Aktumsek, Abdurrahman; Boroja, Tatjana; Stanic, Snezana
    In the present study, the methanolic extracts of Cytisopsis dorycniifolia and Ebenus hirsuta were analyzed for antioxidant, enzyme inhibitory, antimicrobial and antigenotoxic activities. The identification and quantification of phenolic compounds in these extracts were performed using liquid chromatography coupled with mass spectrometry (LC-MS/MS) technique, and the main components were hyperoside, rutin, hesperidin, tannic acid and p-coumaric acid. The extracts had remarkable biological effects. Generally, C. dorycniifolia had higher level of bioactive components and exhibited stronger in vitro antioxidant effects than E. hirsuta. E. hirsuta was active especially against bacteria Pseudomonas fluorescens and Agrobacterium tumefaciens and fungi Puncturella fastigiata, while C dorycniifolia was active against bacteria E. faecalis and had moderate activity against Trichoderma species. The extracts displayed strong antigenotoxic effects (98.6% for E. hirsuta and 76.3% for C dorycniifolia). Our findings suggested that C dorycniifolia and E. hirsuta could be beneficial for the preparation of high-value pharmaceuticals and functional food ingredients. (C) 2016 Elsevier B.V. All rights reserved.
  • Küçük Resim Yok
    Öğe
    Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey
    (Hindawi Ltd, 2015) Zengin, Gokhan; Guler, Gokalp Ozmen; Aktumsek, Abdurrahman; Ceylan, Ramazan; Picot, Carene Marie Nancy; Mahomoodally, M. Fawzi
    We aimed to investigate the inhibitory potential of three medicinal plants (Hedysarum varium, Onobrychis hypargyrea, and Vicia truncatula) from Turkey against key enzymes involved in human pathologies, namely, diabetes (alpha-amylase and alpha-glucosidase), neurodegenerative disorders (tyrosinase, acetylcholinesterase, and butyrylcholinesterase), and hyperpigmentation (tyrosinase). The antioxidant potential, phenolic and flavonoid content of ethyl acetate, and methanolic and aqueous extracts were investigated using in vitro assays. The total antioxidant capacity (TAC), beta-carotene/linoleic acid bleaching activity, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH center dot), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)), cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), and metal chelating activity on ferrous ions were used to evaluate the antioxidant capabilities of the extracts. The half-maximal inhibitory concentrations (IC50) of the extracts on cholinesterase, tyrosinase, and alpha-amylase were significantly higher than the references, galantamine, kojic acid, and acarbose, respectively. The half-maximal effective concentrations (EC50) of the extracts on TAC, CUPRAC, and FRAP were significantly higher than trolox. The phenol and flavonoid contents of the plant extracts were in the range 20.90 +/- 0.190-83.25 +/- 0.914mg gallic acid equivalent/g extract and 1.45 +/- 0.200-39.71 +/- 0.092mg rutin equivalent/g extract, respectively. The plants were found to possess moderate antioxidant capacities and interesting inhibitory action against key enzymes.
  • Küçük Resim
    Öğe
    In vitro multiple pharmacological targets of Colutea cilicica Boiss. & Balansa against key enzymes linked to neurodegenerative diseases, diabetes, and hyperpigmentation
    (2018) Uysal, Şengül; Ceylan, Ramazan; Abdurrahman , Aktumsek; Güler, Gökalp Özmen; Picot, Carene; Zengin, Gökhan
    Prevention and treatment of noncommunicable diseases such as neurodegenerative diseases, diabetes, and hyperpigmentationusing medicinal plants has attracted increasing attention during the past few decades. In this study, Colutea cilicicaBoiss. & Balansa extracts (ethyl acetate, methanol, and water) were evaluated against key enzymes involved in neurodegenerativediseases, diabetes, and hyperpigmentation. The antioxidant (free radical scavenging, reducing power, ?-carotene/linoleic acid, and phosphomolybdenum) and metal chelation properties were also investigated. The methanol extracts of C.cilicica vigorously inhibited the activities of acetylcholinesterase and butyrylcholinesterase (1.33 and 0.68 mg galantamineequivalents (GALAE)/g extract, respectively). It was observed that C. cilicica extracts possessed a higher inhibitory potentialfor ?-glucosidase (2.71–1.23 mmol acarbose equivalents (ACAE)/g extract) than that for ?-amylase (0.57–0.12 mmol ACAE/gextract). The water extract of C. cilicica showed potent radical scavenging capacity against DPPH (2, 2-diphenyl-1-picrylhydrazyl)and ABTS (2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (42.46 and 57.70 mg trolox equivalents (TE)/g extract,respectively). Phytochemical determination showed that C. cilicica water extract (17.26 mg rutin equivalents (RE)/g extract)was rich in flavonoids compared with ethyl acetate and methanol extracts (2.78 and 2.83 mg RE/g extract, for the respectiveextracts). These findings reveal the interesting potential of C. cilicica as a valuable source of phytochemicals that can be usedagainst common noncommunicable diseases, particularly against enzymes involved in neurodegenerative diseases.
  • Küçük Resim Yok
    Öğe
    Shedding light on the biological and chemical fingerprints of three Achillea species (A-biebersteinii, A-millefolium and A-teretifolia)
    (Royal Soc Chemistry, 2017) Zengin, Gokhan; Aktumsek, Abdurrahman; Ceylan, Ramazan; Uysal, Sengul; Mocan, Andrei; Guler, Gokalp Ozmen; Mahomoodally, M. Fawzi
    Representatives of the Achillea genus are widely used as foods or nutraceuticals. Considering the increasing demand for herbal dietary supplements with health promoting effects, the objective of this research was to evaluate the biological and chemical profiles of different extracts (ethyl acetate, methanol and water) obtained from three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). The antioxidant (free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum), enzyme inhibitory (anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glucosidase) and antimicrobial (antibacterial and antifungal) effects were assessed to investigate their biological profiles. Moreover, the total phenolic and flavonoid contents were determined and LC-MS analysis was performed for the chemical profile of the investigated extracts. The LC-MS analysis revealed the presence of several caffeoylquinic acids in these extracts. Generally, the methanol and water extracts exhibited stronger antioxidant abilities, which correlated with the higher levels of phenolic compounds when compared to the ethyl acetate extracts. In addition, the best antimicrobial activities were obtained for the ethyl acetate and methanolic extracts. However, the ethyl acetate extract had remarkable enzyme inhibitory potential. On the basis of our results, Achillea species may be promoted as promising sources of natural agents and used for the development of nutraceuticals or functional food ingredients.
  • Küçük Resim Yok
    Öğe
    Traditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L-czeczottianus and L-nissolia
    (Frontiers Media Sa, 2017) Llorent-Martinez, Eulogio J.; Zengin, Gokhan; Cordova, Maria L. Fernandez-De; Bender, Onur; Atalay, Arzu; Ceylan, Ramazan; Mollica, Adriano
    Members of the genus Lathyrus are used as food and as traditional medicines. In order to find new sources of biologically-active compounds, chemical and biological profiles of two Lathyrus species (L. czeczottianus and L. nissolia) were investigated. Chemical profiles were evaluated by HPLC-ESI-MSn, as well as by their total phenolic and flavonoid contents. In addition, antioxidant, enzyme inhibitory, and cytotoxic effects were also investigated. Antioxidant properties were tested by using different assays (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation). Cholinesterases (AChE and BChE), tyrosinase, alpha-amylase, and alpha-glucosidase were used to evaluate enzyme inhibitory effects. Moreover, vitexin (apigenin-8-C-glucoside) and 5-O-caffeoylquinic acid were further subjected to molecular docking experiments to provide insights about their interactions at molecular level with the tested enzymes. In vitro cytotoxic effects were examined against human embryonic kidney cells (HEK293) by using iCELLigence real time cell analysis system. Generally, L. czeczottianus exhibited stronger antioxidant properties than L. nissolia. However, L. nissolia had remarkable enzyme inhibitory effects against cholinesterase, amylase and glucosidase. HPLC-ESI-MSn analysis revealed that flavonoids were major components in these extracts. On the basis of these results, Lathyrus extracts were rich in biologically active components; thus, these species could be utilized to design new phytopharmaceutical and nutraceutical formulations.