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Öğe Endometrial CD56+natural killer cells in women with recurrent implantation failure: An immunohistochemical study(Galenos Yayincilik, 2020) Babayeva, Gulchin; Purut, Yunus Emre; Giray, Burak; Oltulu, Pembe; Alakus, Rabia; Colakoglu, Mehmet CengizObjective: Implantation failure is a multifactorial problem of reproductive medicine. However, the mechanism of this process is still not fully understood. There is increasing evidence that these cases of recurrent implantation failure might have an immunologic background. Uterine natural killer (NK) cells provide immune-modulation at the interface between maternal decidua and the trophoblast. The aim of this study to evaluate whether there was a significant difference in the number of endometrial CD56+ NK between women with a history of recurrent implantation failure and women who had a live birth. Materials and Methods: Patients with a history of recurrent implantation failure were included in the study. Twenty-five women with a history of recurrent implantation failure were assigned to the case group, and 25 women who had one or more live births were assigned to the control group. Endornetrial biopsies were obtained during the luteal phase on the 21st-24th clay of the menstrual cycle. Results: There was a statistically significant difference between the groups concerning the number of deliveries (p<0.001) and miscarriages (p<0.001). The mean number of uNK was 10.5 +/- 10.5 cells/mm(2) in the case group and 19.2 +/- 11.2 cells/mm(2) in the control group. There was a statistically significant difference between the two groups (p=0.003). Conclusion: Implantation failure is a multifactorial problem of reproductive medicine. The results of our study suggest that uterine NK play a role in the progress of normal pregnancy and reduced uterine NK cell numbers were associated with implantation failure.Öğe The evaluation of recombinant LH supplementation in patients with suboptimal response to recombinant FSH undergoing IVF treatment with GnRH agonist down-regulation(Taylor & Francis Ltd, 2015) Yilmaz, Fatma Yazici; Gorkemli, Huseyin; Colakoglu, Mehmet Cengiz; Aktan, Murat; Gezginc, KazimWe aimed to evaluate the clinical efficacy of r-LH supplementation to r-FSH in patients with suboptimal response to ovarian stimulation undergoing assisted reproduction with GnRH-a downregulation and stimulation with r-FSH. One-hundred thirty-seven patients were included in the study; among them 52 showed normal ovarian response to stimulation and composed the control group (Group 1), and 85 showed suboptimal response to stimulation and were divided into two groups. For Group 2 (n = 50), 75 IU/L r-LH was added to the treatment, for Group 3 (n = 35) r-FSH dose was increased by 75 IU/L. IVF results were compared between the groups. Implantation rates were 34.8% in control group, and 36.1% and 15% in LH supplementation group and increased-dose r-FSH group, respectively. Implantation rates were statistically significantly higher in Groups 1 and 2 compared to Group 3 (p < 0.02). Pregnancy rate was noticed in 64.7% of Group 1, 57.8% of Group 2 and at 32.4% of Group 3. Pregnancy rate was significantly higher in Group 2 than Group 3 (p < 0.05). r-LH supplementation is an option for improving IVF outcome in patients with suboptimal ovarian response to ovulation induction with r-FSH during GnRH agonist down-regulation. Particularly, r-LH is recommended as it may have a beneficial action on implantation in selected group.Öğe Investigation of peripheral natural killer cell activity in recurrent miscarriages of unknown cause(Bayrakol Medical Publisher, 2023) Alyazova, Elnare; Babayeva, Gulchin; Colakoglu, Mehmet Cengiz; Horasanli, Jule EricAim: Recent studies on alloimmune factors have shown that Natural Killer (NK) cells may have a role in the implantation and maintenance of pregnancy. It is unclear precisely what kind of relationship exists between uterine and peripheral blood NK cells. Our study aims to investigate the relationship between pNK cells and recurrent pregnancy loss. Material and Methods: Among the patients who applied to the outpatient clinic at University Hospital, retrospectively, women with two or more pregnancy losses constituted the RM group, and women with two or more live births and no miscarriage formed the control group. Two or more miscarriages before 20 weeks of gestation were accepted as the criterion to consider a case of recurrent pregnancy loss. Results: To investigate the etiology of miscarriage in RM patients, parameters associated with the cytotoxicity of pNK cells in women with RM and control fertile women were evaluated. Although the median pNK activity level was relatively higher in the recurrent low group compared to the control group, there was no significant difference between the groups in our study (p=0.448). Discussion: The present study found no significant difference in the percentages of CD56+dim and CD56+bright pNK cells between the patient group with unexplained RM and the healthy fertile control group. There was also no significant difference in CD8 and CD158a expressions in pNK cells between the patient group with unexplained RM and the healthy fertile control group.Öğe Role of ChREBP and SREBP-1c in gestational diabetes: two key players in glucose and lipid metabolism(Springer India, 2023) Eroglu, Nurgul; Yerlikaya, Fatma Humeyra; Onmaz, Duygu Eryavuz; Colakoglu, Mehmet CengizBackground Gestational diabetes mellitus (GDM) is any degree of glucose intolerance diagnosed during pregnancy. Metabolic abnormalities in GDM are associated with pancreatic beta-cell destruction and decreased insulin sensitivity. Carbohydrate-responsive element-binding protein (ChREBP) and sterol regulatory element-binding protein-1c (SREBP-1c) are two transcription factors that play a key role in carbohydrate and lipid metabolism homeostasis. Our aim in this study was to elucidate the role of these proteins in GDM. Methods The study included 50 pregnant women diagnosed with GDM and 52 pregnant women with normal glucose tolerance. Maternal blood samples were collected from pregnant women between the 24th and 28th weeks of pregnancy. Serum SREBP-1c and ChREBP levels were measured with commercial ELISA kits according to the manufacturer's instructions. Results Serum ChREBP (p=0.044) and SREBP1c (p=0.042) levels of pregnant women with normal glucose tolerance were found to be statistically significantly higher than women with GDM.
