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Öğe Investigation of CCL1 rs159294 T/A gene polymorphism in pulmonary and extrapulmonary tuberculosis patients(Turkish Assoc Tuberculosis & Thorax, 2013) Ozdemir, Fethi Ahmet; Erol, Deniz; Yuce, Hueyin; Konar, Vahit; Senli, Ebru Kara; Bulut, Funda; Deveci, FigenIntroduction: The purpose of this study is to reveal whether CCL1 rs159294 T/A polymorphism in pulmonary and extrapulmonary tuberculosis patients pose a risk to catch tuberculosis or not. Materials and Methods: In the study, peripheral blood samples from the control group, which includes 160 patients, who consulted to Firat University Faculty of Medicine, Pulmonology Policlinic in Elazig province and who were diagnosed with tuberculosis; and 160 healthy individuals, were taken and put into tubes containing EDTA. Each tube contained 2 cc blood samples. DNA isolation was made from these blood samples and CCL1 rs159294 T/A polymorphism was defined with PCR-RFLP analysis. Results: For CCL1 rs159294 T/A polymorphism, TT genotype was found in 98 (61.3%) patients, TA genotype was found in 58 (36.3%) patients, AA genotype was found in 4 (2.5%) patients among 160 patients with tuberculosis; and TT genotype was found in 50 (70.4%) patients, TA genotype in 20 (28.2%) patients, AA genotype was found in 1 (1.4%) patient among 71 patients with pulmonary tuberculosis; TT genotype was found in 48 (53.9%) patients TA genotype was found in 38 (42.7%) patients and AA genotype was found in 3 (3.4%) patients among 89 extrapulmonary tuberculosis patients. And in control group, among 160 healthy individuals, TT genotype was found in 100 (62.5%) individuals, TA genotype was found in 58 (36.3%) individuals, AA genotype was found in 2 (1.3%) individuals and no statistically significant difference was found. Conclusion: CCL1 rs159294 T/A polymorphism do not form an inclination to tuberculosis in our population.Öğe Lack of Association of 1513 A/C Polymorphism in P2X7 Gene with Susceptibility to 7 Pulmonary and Extrapulmonary Tuberculosis(Turkish Assoc Tuberculosis & Thorax, 2014) Ozdemir, Fethi Ahmet; Erol, Deniz; Konar, Vahit; Yuce, Hueyin; Senli, Ebru Kara; Bulut, Funda; Deveci, FigenIntroduction: Tuberculosis, is one of the a leading causes of death worldwide, is characterized by different clinical forms including: latent, localized pulmonary infection and extrapulmonary tuberculosis. Candidate gene association studies have implicated common polymophisms in genes that may influence the development of tuberculosis. This study, aimed to elucidate the role of P2X(7) gene in 1513A/C polymorphism the etiopathogenesis of tuberculosis. Materials and Methods: The study included 160 patients with tuberculosis (71 pulmonary and 89 extrapulmonary tuberculosis) and 160 healthy controls. Genomic DNA was isolated and 1513A/C polymorphism in P2X(7) gene was genotyped by PCR-RFLP method. Results: Frequency of P2X(7) AA genotype was 47.5% in controls and 56.87% in patients, AC frequency was 39.37% controls and 32.5% in patients, CC genotype was 13.12% in controls and 10.62% in patients. No significant difference in allele and genotype frequencies (1513A/C polymorphism) between tuberculosis patients and controls was found. Conclusion: The results suggest that 1513A/C polymorphism of P2X(7) gene is not associated with pulmonary and extrapulmonary tuberculosis in the Eastern Turkey.Öğe Lack of Association of 1513 A/C Polymorphism in P2X7 Gene with Susceptibility to Pulmonary and Extrapulmonary Tuberculosis(2014) Özdemir, Fethi Ahmet; Erol, Deniz; Konar, Vahit; Yüce, Hüseyin; Karaşenli, Ebru; Bulut, Funda; Deveci, FigenGiriş: Latent, pulmoner ve ekstrapulmoner şeklinde farklı klinik formları olan tüberküloz, dünya genelinde ölüme sebebiyet veren hastalıkların en önemlilerinden biridir. Aday genlerle yapılan çalışmalarda yaygın polimorfizmlerin tüberkülozun gelişmesinde etkili olabileceği sonucuna varılmıştır. Bu çalışmada P2X7 genindeki 1513 A/C polimorfizminin tüberkülozun etyopatogenezisindeki rolünün ortaya çıkarılması amaçlanmıştır. Materyal ve Metod: Bu çalışma 160 tüberkülozlu (71 pulmoner tüberkülozlu ve 89 ekstrapulmoner tüberkülozlu) 160 da sağlıklı bireyi kapsamaktadır. Genomik DNA izolasyonu gerçekleştirildikten sonra P2X7 genindeki 1513 A/C polimorfizmi PCR-RFLP metoduyla genotiplendirildi. Bulgular: AA genotipinin frekansı kontrol grubunda %47.5, hastalarda %56.87, AC genotipinin frekansı kontrol grubunda %39.37, hastalarda %32.5, CC genotipinin frekansı kontrol grubunda %13.12, hastalarda %10.62 olarak saptandı. Hastalar ile kontrol grubunun allel ve genotip frekansları arasında anlamlı bir farklılık bulunamadı. Sonuç: Sonuçlarımız Türkiye’nin doğu bölgesinde P2X7 genindeki, 1513 A/C polimorfizminin pulmoner ve ekstrapulmoner tüberküloz hastalığıyla ilişkisinin olmadığını gösterdi.Öğe Pulmoner ve ekstrapulmoner tüberküloz hastalarında CCL1 rs159294 T/A gen polimorfizminin araştırılması(2013) Özdemir, Fethi Ahmet; Erol, Deniz; Yüce, Hüseyin; Konar, Vahit; Kara Şenli, Ebru; Bulut, Funda; Deveci, FigenGiriş: Bu çalışmada amaç, pulmoner ve ekstrapulmoner tüberküloz hastalarındaki, CCL1 rs159294 T/A polimorfizminin tüberküloza yakalanma riski oluşturup oluşturmadığını ortaya koymaktır. Materyal ve Metod: Çalışmamızda Elazığ ili yöresinde Fırat Üniversitesi Tıp Fakültesi Göğüs Hastalıkları Polikliniğine başvuran, tüberküloz teşhisi konulmuş 160 hasta ve 160 sağlıklı bireyden oluşan kontrol grubuna ait kişilerden periferal kan örnekleri alınarak EDTA içeren tüplere 2 cc olacak şekilde konulmuştur. Bu kan örneklerinden DNA izolasyonu gerçekleştirilerek CCL1 rs159294 T/A polimorfizmi PCR-RFLP analizi yapılarak belirlenmiştir. Bulgular: CCL1 rs159294 T/A polimorfizmi için 160 tüberkülozlu hastanın 98 (%61.3)’inde TT genotipi, 58 (%36.3)’inde TA genotipi, 4 (%2.5)’ünde de AA genotipi, 71 pulmoner tüberkülozlu hastanın 50 (%70.4)’sinde TT genotipi, 20 (%28.2)’sinde TA genotipi, 1 (%1.4)’inde de AA genotipi, 89 ekstrapulmoner tüberkülozlu hastanın 48 (%53.9)’inde TT genotipi, 38 (%42.7)’inde TA genotipi, 3 (%3.4)’ünde de AA genotipi tespit edilmiştir. Kontrol grubunda ise 160 sağlıklı bireyin 100 (%62.5)’ünde TT genotipi, 58 (%36.3)’inde TA genotipi, 2 (%1.3)’sinde de AA genotipi belirlenmiş olup, istatistiksel olarak anlamlı bir farklılık saptanamamıştır. Sonuç: CCL1 rs159294 T/A polimorfizmi, popülasyonumuz da tüberküloz hastalığına yatkınlık oluşturmamaktadır
