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    Effect of aqueous extract, hydrosol, and essential oil forms of some endemicOriganumL. (Lamiaceae) taxa on polyphenol oxidase activity in fresh-cut mushroom samples
    (Wiley-Hindawi, 2020) Tanhas, Esra; Martin, Esra; Korucu, Emine Nedime; Dirmenci, Tuncay
    OriganumL. is one of the high-value medicinal and aromatic plants generally used for various purposes in the field of food and health. This study evaluated the effect of aqueous extract, hydrosol and essential oil forms of sevenOriganumtaxa including three endemic species in fresh-cut mushroom samples in order to prevent polyphenol oxidase (PPO) activity. Food samples were treated with these natural preservatives (aqueous extract, hydrosol, and essantial oil) and stored at 4 degrees C temperature for a maximum of 5 days. It was found that the extract form ofOriganum vulgareL. subsp.hirtum(Link) Ietsw. provided maximum PPO inhibition with 64.50% of reduction on 3rd day. Similarly, 3-fold reduction in hydrosol application was observed withOriganum bilgeriP. H. Davis which is the endemic plant. These results were conducted with the extract forms containing water are more effective than essential oil form to inhibit PPO activity. Practical applications In this study, we have investigated alternative usage of someOriganumplants as natural anti-browning food additives in mushroom samples. The samples separately treated with aqueous extract, hydrosol, and essential oil forms of differentOriganumplants were stored until 5 days. PPO activities of samples on 1st, 3rd, and 5th days were used to determinate the shelf life of mushrooms associating with their browness.
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    EFFECTS OF CURCUMIN TREATMENT ON CELL ENERGY STATUS, LEVELS OF MITOCHONDRIAL ENZYMES, AND GENE EXPRESSION OF GLUCOSE-RELATED MECHANISM IN PANCREATIC CANCER CELL LINES
    (Dokuz Eylul Univ Inst Health Sciences, 2023) Korucu, Emine Nedime; Menevse, Esma; Kaya, Dudu Erkoc; Gokturk, Fatma; Arikoglu, Hilal
    Purpose: Curcumin is an active component of turmeric, has antitumor, immunomodulatory, anti-inflammatory effects. This study aimed to investigate the effects of the administration of curcumin on the energy metabolism, the abnormal redox defense mechanism profile, the malignant transformation indicator of Panc-1 and BxPC-3 pancreatic cancer cells.Material and Methods: BxPC-3 and Panc-1 cells were treated with various concentrations of curcumin (20 mu M, 40 mu M, 50 mu M, 60 mu M, 80 mu M, 100 mu M, and 125 mu M ) for 24 h. Cell lysate Adenosine triphosphate (ATP), Adenosine diphosphate (ADP), Adenosine monophosphate (AMP), Manganese superoxide dismutase (MnSOD), and cytochrome p450 reductase (CPR) concentrations were analyzed with HPLC and ELISA methods. Genes expression of Lactate dehydrogenase (LDH), mitochondrially encoded ATP synthase membrane subunit 6 (MTATP6), Glucose transporter 1 (GLUT1), and cytochrome p450 were analyzed by real-time quantitative PCR.Results: IC50 values for 24 hours were found as 47.26 mu M in BxPC-3 and 45.84 mu M in Panc-1 cells. Treatment with curcumin inhibited oxidative stress by increasing MnSOD enzyme levels. ATP levels did not change in BxPC-3 cells, but they increased in Panc-1 supplemented with curcumin. Gene expression of GLUT-1 was significantly down regulated when using at 45 mu M concentration of curcumin, which also affected glucose consumption in both cells. Conclusion: Curcumin showed anti-proliferative and antioxidant effects.
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    Evaluation of sialic acid, malondialdehyde and glutathione levels in infertile male
    (Elsevier Espana Slu, 2022) Menevse, Esma; Dursunoglu, Duygu; Cetin, Nihal; Korucu, Emine Nedime; Erbayram, Fatma Zehra
    Introduction and objectives: Around 15% of productive couples in the world are infertile. Recent years, biochemical mechanisms leads to male infertility are started to research. Redox regulation and oxidative stress (OS) show importance in the pathogenesis of infertility in male. Malondialdehyde (MDA) and Glutathione (GSH) are biochemical indicatives of sperm damage by reactive oxygen species (ROS). In addition, sperm are coated with a thick glycocalyx rich in sialic acids. It is aimed to determine and evaluate the differences between normozoosper-mic and oligozoospermic individuals according to sialic acid, MDA and GSH concentrations and correlations between spermyogram and these parameters. Material and methods: This study was carried out on seminal plasma of individuals who admit-ted to Selcuk University Faculty of Medicine IVF Unit Andrology Laboratory. The groups were divided into two as normozoospermics (n = 30, sperm concentration >= 15 million/mL), and oligozoospermics (n = 30, sperm concentration < 15 million/mL). Spermyogram were evaluated regarding WHO (2010) Kruger criteria. GSH, MDA and sialic acid concentrations were analyzed in seminal plasma. Diagnostic performance of sialic acid has been determined with ROC curve analysis. Results: Sialic acid levels were significantly lower in Normozoospermic than Oligozoosper-mic individuals (p < 0.0001), MDA and GSH levels were not differ significantly in both groups (p > 0.05). Sialic acid correlated significantly with most of the spermyogram findings. When diagnostic performance of sialic acid was evaluated, the cut off value of sialic acid found as 4.175 nmol/mL by ROC curve. Conclusion: High seminal plasma sialic acid levels may be used as a biomarker and sialic acid is important determinant in oligozoospermia. (c) 2022 Asociacion Espan tilde ola de Andrologi acute accent a, Medicina Sexual y Reproductiva. Published by Else-vier Espan tilde a, S.L.U. All rights reserved.
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    In vitro antifungal and antibiofilm activities of novel sulfonyl hydrazone derivatives against Candida spp.
    (Masson Editeur, 2023) Aydin, Merve; Ozturk, Ali; Duran, Tugce; Ozmen, Ummuhan Ozdemir; Sumlu, Esra; Ayan, Esra Bilen; Korucu, Emine Nedime
    Background: The aim of this study was to investigate the antifungal and antibiofilm activity of the new sulfo-nyl hydrazones compound derived from sulphonamides.Methods: In this study, new sulfonyl hydrazone series were synthesized via a green chemistry method. The structures of the synthesized compounds were characterized by elemental analyses and spectroscopic meth-ods. The antifungal activities of the Anaf compounds against Candida strains under planktonic conditions were tested. The biofilm-forming ability of Candida strains was determined and the inhibitory effects of Anaf compounds on Candida biofilms compared with fluconazole were measured by MTT assay. Expression analy-sis of biofilm-related genes was investigated with qRT-PCR. The statistical analysis was performed using a one-way ANOVA test.Candida: strains was determined and the inhibitory effects of Anaf compounds on Candida biofilms compared with fluconazole were measured by MTT assay. Expression analysis of biofilm-related genes was investigated with qRT-PCR. The statistical analysis was performed using a one-way ANOVA test. Results: A total of 16 (45.7%) out of 35 Candida isolates were determined as strong biofilm producers in this study. C. albicans was the most biofilm producer, followed by C. krusei and C. lusitaniae. The Anaf compounds had a broad spectrum of activity with MIC values ranging from 4 mg/ml to 64 mg/ml. Our data indicated that the Anaf compound had a significant effect on inhibiting biofilm formation in both fluconazole-susceptible and-resistant strains. The expression levels of hypha-specific genes als3, hwp1, ece1 and sap5 were downre-gulated by Anaf compounds.Conclusions: Our study revealed that the Anaf compounds had antifungal activity and inhibited fungal bio-films, which may be related to the suppression of C. albicans adherence and hyphal formation. These results suggest that Anaf compounds may have therapeutic potential for the treatment and prevention of biofilm-associated Candida infections.(c) 2022 SFMM. Published by Elsevier Masson SAS. All rights reserved.
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    Investigation of the effects of the toll-like receptor 4 pathway on immune checkpoint vista in pancreatic cancer
    (Springer, 2022) Topcu, Kubra Sena Bas; Korucu, Emine Nedime; Menevse, Esma; Kocak, Nadir; Duran, Tugce
    Pancreatic ductal adenocarcinoma (PDAC) is one of the most common malignant tumors of the pancreas. Preclinical studies show that it evades the immune system with immune checkpoints and promotes tumor development. V-domain Ig suppressor of T cell activation (VISTA) is a new immune-check point from the B7 family and is highly expressed in cancer cells. Overexpression of toll like receptor 4 (TLR4) in pancreatic adenocarcinoma is associated with induced tumorigenesis, tumor growth, resistancy to chemotherapy. Naloxone is an opioid and inhibits TLR4-ligand association. In this study, we investigated the relation of TLR4 and downstream pathways with immune-check point VISTA in pancreatic cancer proliferation. We initially collected pancreatic cancer-related datasets using the GEPIA2 and UALCAN databases. Based on this data obtained the effect of various concentrations and incubation times of naloxone were used on PANC-1 cells proliferation. A combination of naloxone and VISTA-siRNA were applied, and the effect of both naloxone and combined treatment on TLR4, Interleukin 1 receptor associated kinase 4 (IRAK4) and VISTA gene expression were analyzed in pancreatic cancer cells. As a result of analysis with Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), gene expression levels of TLR4, IRAK4 and VISTA were significantly suppressed and cell proliferation was significantly reduced. We found that administration of naloxone and VISTA-siRNA in combination with PDAC cells suppressed signaling. Therefore, we considered that the relationship between VISTA and TLR4 signaling pathways and the other possible associated signal molecules may be an important marker in determining the response of immune checkpoint inhibitors in cancer treatment.
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    THE LEVELS OF ADENOSINE TRIPHOSPHATE SYNTHASE 6, MANGANESE SUPEROXIDE DISMUTASE, NADPH-CYTOCHROME P450 REDUCTASE, LACTATE DEHYDROGENASE IN SEMINAL PLASMA OF OLIGOZOOSPERMIA AND NORMOZOOSPERMIA MEN
    (Nobel Ilac, 2023) Menevse, Esma; Dursunoglu, Duygu; Korucu, Emine Nedime; Cetin, Nihal; Erbayram, Fatma Zehra
    Objective: In recent years, energy production and enzymes in mitochondrial pathways are an important in male fertilization.Material and Method: For this purpose, we tried to determine the relationship between normozoospermia and oligozoospermia individuals according to levels of some mitochondrial enzymes. The seminal plasma levels of mitochondrial encoded adenosine triphosphate synthase 6 (mtATP6), manganese superoxide dismutase (Mn-SOD), NADPH-cytochrome P450 reductase (CPR), lactate dehydrogenase (LDH) were analyzed and assessed their correlations between data of spermogram. The study was composed of two groups consisting of normozoospermic and oligozoospermic volunteers.Results: mtATP6 levels were significantly higher in normozoospermia (0.97 & PLUSMN;0.50 ng/mg protein, p=0.008) than oligozoospermia (0.64 & PLUSMN;0.32 ng/mg protein). LDH levels were higher in normozoospermia (0.25 & PLUSMN;0.074 U/ mg protein, p=0.007) than oligozoospermia (0.199 & PLUSMN;0.049 ng/mg protein). The groups did not differ in terms of MnSOD and CPR levels. mtATP6 levels were significantly correlated with sperm concentration, total number and total motility, immotility, total progressive motile sperm count (TPMSC) and long head anomaly. LDH levels were related with long head and short tail anomaly.Conclusion: We observed some of the spermatogenetic anomalies are caused by defects in energy regulation. We did not find any evidence on MnSOD and CPR activity regarded as determinative parameters in response to increased oxidative stress in oligozoospermia.
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    Regenerative effects of hyperbaric oxygen therapy and platelet-rich plasma on the osteochondral defects of rats
    (Turkish Joint Diseases Foundation, 2020) Korucu, Ismail Hakki; Kekec, Ahmet Fevzi; Arslan, Abdullah; Oltulu, Pembe; Korucu, Emine Nedime; Ozer, Mustafa
    Objectives: This study aims to investigate the effects of hyperbaric oxygen (HBO) therapy and platelet-rich plasma (PRP) on the regeneration of osteochondral defects of the rats, and the synergistic effect of this combined treatment. Materials and methods: This randomized, controlled, and interventional animal study was conducted between May 2014 and August 2014 Osteochondral regeneration was evaluated in four treatment groups (control, PRP, HBO, and HBO+PRP groups) at the 30 th day after iatrogenic injury. Thirty-two female Wistar albino rats (weighing 248-305 g) underwent arthrotomy and osteochondral surgery on left knees. The regenerations of defects were then examined histologically by the modified version of O'Driscoll score. Results: Groups that were treated with either HBO or PRP alone regenerated significantly better than the control group (p=0.01), while no significant difference was found between the HBO- and PRP-treated groups (p 0.05). The defects in group 4 (treated with both HBO and PRP) regenerated significantly better than the control group, the HBO-treated group alone, and the PRP-treated group alone (p=0.01). Conclusion: The results of this study showed a synergistic effect of HBO and PRP on knee cartilage regeneration. However, the possible underlying mechanisms should be the subject of future researches. The aggregation and activation of growth factors released from platelets whose activation is increased in the hyperbaric environment may explain this effect. This may result in a better regeneration than the effect of PRP or HBO alone.