Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study
Yükleniyor...
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: The purpose of this study was to investigate the effects of the omentum, peritoneum, paratenon and skeletal muscle on the proliferation of the cartilage tissue using rabbit model as an in vivo
culture medium.
Methods: 6 months old forty-five New Zealand rabbits were randomized into omentum, peritoneum,
muscle, and Achilles paratenon groups. Standard sized osteochondral grafts were harvested from right
knees and immediately placed into the specified tissues. Control group was fresh cartilage at the end of
follow-up. After five months, samples were collected and evaluated macroscopically by measuring their
dimensions (vertical ¼ D1, horizontal ¼ D2, and depth ¼ D3) and volumes, and histologically by counting
the chondrocyte number using camera lucida method.
Results: Macroscopically, increase in mean values for D1 and D2 dimensions of specimens from paratenon and omentum compared to pretransplant dimensions was statistically significant (p < 0.05).
Although, volume measurements were higher in omentum and peritoneum group compared to pretransplant dimensions, increase was not significant (p > 0.05). Histologically, mean chondrocyte count
was 14.0 ± 0.6 in fresh articular cartilage. Mean chondrocyte counts were 14.4 ± 0.9 in omentum group,
15.4 ± 1.0 in peritoneum group, 9.7 ± 1.3 in muscle group and 9.2 ± 0.4 in Achilles paratenon group
respectively. However, mean chondrocyte counts were higher in samples of omentum and peritoneum
group compared to fresh articular cartilage, increase was not statistically significant (p > 0.05).
Discussion: Transplantation of the cartilage grafts into mesothelium enhanced the chondrocyte counts
and volumes compared with the pretransplant measurements. Mesothelium may have the potential to
be used as an in vivo culture medium for osteochondral tissue growth.
Açıklama
WOS:000392918000010
PubMed ID:27717559
PubMed ID:27717559
Anahtar Kelimeler
Cerrahi, Cartilage preservation, Mesothelium, Chondrocyte, Knee
Kaynak
Acta Orthopaedica et Traumatologica Turcica
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
50
Sayı
5
Künye
Bilge, O., Büyükdoğan, K., Doral, M. N., Turhan, E., Huri, G., Sargon, M. F. (2016). Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study. Acta Orthopaedica Et Traumatologica Turcica, 50, 5, 539-543.