Otoimmün hastalıklarda antinötrofil sitoplazmik antikorların araştırılması
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Dosyalar
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Antinötrofil sitoplazmik antikorlar, monositlerin lizozomları ve nötrofillerin sitoplazmik granüllerinde bulunan antijenlere karşı oluşan otoantikorlardır. Özellikle Wegener granulomatozis olmak üzere pek çok sistemik vaskülitte tanı değeri olan değerli bir prognostik belirteçtir. Çalışmada 2009 yılında otoimmün hastalık ön tanısı alan hastaların ANCA test sonuçlarının retrospektif olarak irdelenmesi amaçlanmıştır. Yöntem: Bu çalışmada hastanemizde 2009 yılında otoimmün hastalık ön tanısıyla Mikrobiyoloji Laboratuvarına gönderilen 1040 hastanın serum örneklerinde antinötrofil sitoplazmik antikorlar indirekt immunfloresans yöntemi ile incelenmiş, hedef antijenleri ve nötrofillerde oluşturdukları paternlere göre sitoplazmik (cANCA) veya perinükleer (pANCA) varlığı kalitatif olarak belirlenmiştir. Bulgular: Toplam 1040 hastanın 44’ünde (% 4,2) antinötrofil sitoplazmik antikorlar pozitif olarak bulunmuştur. Bunlar içinde 34’ü (% 3,2) pANCA, 10’u (% 1,0) cANCA’dır. Bu 44 hastanın 10’u (% 22,7) kronik akciğer hastalığı, 5’i (% 11,3) bağ dokusu hastalığı, 5’i (% 11,3) artrit, 5’i (% 11,3) sistemik hastalıklar, 4’ü (% 9,09) romatoid artrit, 4’ü (% 9,09) vaskülit, 4’ü (% 9,09) iridosiklit, 2’si (% 4,5) sistemik lupus eritematosus (SLE), 2’si (% 4,5) mikroskopik polianjitis, 2’si (% 4,5) ülseratif kolit, 1’i (% 2,2) lokalize skleroderma tanısı almıştır. Sonuç: ANCA pozitifliğinin özellikle vaskülitler başta olmak üzere otoimmün hastalıklarda tanı açısından önemli bir yeri vardır. ANCA tayini klinik tanıya yardımcı olarak araştırılması gereken önemli bir otoantikordur. Elde ettiğimiz veriler, hastanemizde ANCA testlerinin maliyet etkin kullanım politikalarının gözden geçirilmesi gerekliliğini ortaya koymaktadır.
Objective: Anti neutrophil cytoplasmic antibodies (ANCA) are autoantibodies directed against lisosomes of monocytes and antigens present in the cytoplasmic granules of neutrophils. ANCA is a prognostic marker which is typically found in many forms of systemic vasculitis especially Wegener’s granulomatosis. This study was aimed to evaluate retrospective analysis of test results for ANCA in patients presumptive diagnosed with autoimmune disease during the year of 2009. Methods: In this study, 1040 blood sera were collected from patients presumptive diagnosed as autoimmune disease. Sera were tested for anti neutrophil cytoplasmic antibodies (ANCA) with indirect immunofluorescence assay method. ANCA was determined according to their target antigens and indirect immunofluorescence (IIF) patterns that produce on normal neutrophils and assessed qualitatively. Results: 44 (4.2%) of 1040 patients were ANCA positive. Out of 44 patients, 34 (3.2%) of ANCA patients were pANCA positive and 10 (1.0%) were cANCA positive. 10 ( 22.7%) patients have chronic lung disease, 5 (11.3%) have connective tissue disease, 5 (11.3%) have arthritis, 5 (11,3%) have systemic diseases, 4 (9.09%) have vasculitis, 4 (9.09%) have rheumatoid arthritis, 4 (9.09%) have iridocyclitis. 2 (4.5%) patients were diagnosed systemic lupus erythematosus (SLE), 2 (4.5%) have microscopic poliangitis, 2 (4.5%) have ulcerative colitis and 1 (2.2%) patient has localized scleroderma. Conclusion: As the results indicate, the positivity of ANCA in autoimmune disease has important clinical significance. ANCA assays are important in the diagnosis and prognosis of many autoimmune disease especially vasculitis. Our results suggest that cost-effective use of ANCA tests should be reviewed in our hospital.
Objective: Anti neutrophil cytoplasmic antibodies (ANCA) are autoantibodies directed against lisosomes of monocytes and antigens present in the cytoplasmic granules of neutrophils. ANCA is a prognostic marker which is typically found in many forms of systemic vasculitis especially Wegener’s granulomatosis. This study was aimed to evaluate retrospective analysis of test results for ANCA in patients presumptive diagnosed with autoimmune disease during the year of 2009. Methods: In this study, 1040 blood sera were collected from patients presumptive diagnosed as autoimmune disease. Sera were tested for anti neutrophil cytoplasmic antibodies (ANCA) with indirect immunofluorescence assay method. ANCA was determined according to their target antigens and indirect immunofluorescence (IIF) patterns that produce on normal neutrophils and assessed qualitatively. Results: 44 (4.2%) of 1040 patients were ANCA positive. Out of 44 patients, 34 (3.2%) of ANCA patients were pANCA positive and 10 (1.0%) were cANCA positive. 10 ( 22.7%) patients have chronic lung disease, 5 (11.3%) have connective tissue disease, 5 (11.3%) have arthritis, 5 (11,3%) have systemic diseases, 4 (9.09%) have vasculitis, 4 (9.09%) have rheumatoid arthritis, 4 (9.09%) have iridocyclitis. 2 (4.5%) patients were diagnosed systemic lupus erythematosus (SLE), 2 (4.5%) have microscopic poliangitis, 2 (4.5%) have ulcerative colitis and 1 (2.2%) patient has localized scleroderma. Conclusion: As the results indicate, the positivity of ANCA in autoimmune disease has important clinical significance. ANCA assays are important in the diagnosis and prognosis of many autoimmune disease especially vasculitis. Our results suggest that cost-effective use of ANCA tests should be reviewed in our hospital.
Açıklama
Anahtar Kelimeler
Genel ve Dahili Tıp, Antinötrofil Sitoplazmik Antikor, Otoimmün Hastalık, Wegener Granulomatozis, Antineutrophil Cytoplasmic Antibodies, Autoimmune Disease, Wegener Granulomatosis
Kaynak
Genel Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
22
Sayı
1
Künye
Özdemir, M., Feyzioğlu, B., Gündem, N. S., Baykan, M., Baysal, B. (2012). Otoimmün hastalıklarda antinötrofil sitoplazmik antikorların araştırılması. Genel Tıp Dergisi, 22, 1, 1-5.
