Diyabetik ve Diyabetik Olmayan Bireylerde Konjunktiva Florası
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Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Diyabetik ve diyabetik olmayan olgularda konjunktival bakteri florasının incelenmesidir.Gereç ve Yöntem: Elli üç diyabetik hasta ve 43 diyabetik olmayan sağlıklı kişi çalışmaya dahil edildi. Çalışma için katılımcıların sağ göz alt fornikslerinden steril eküvyonla sürüntü alındı. Örnekler mikrobiyoloji laboratuvarında kanlı agar, çikolata agar, eosin metilen mavisi laktoz sukroz agar ve sabouraud %4 dekstroz agara ekildi. İzole edilen mikroorganizmalar rutin mikrobiyolojik yöntemlerle tanımlandı.Bulgular: Bakteri izolasyon oranı diyabetik hastalarda %38,5 ve diyabetik olmayanlarda %34,9 idi. Diyabetik grupta olguların %30'unda Staphylococcus aureus, %20'sinde Escherichia coli, %10'unda koagülaz negatif Staphylococcus, %10'unda Klebsiella pneumoniae ve %30'unda birden fazla bakteri üremesi saptandı. Diyabetik olmayan grupta %53,3'ünde Staphylococcus aureus, %26,7'sinde koagülaz negatif Staphylococcus, %6,7'sinde Klebsiella pneumoniae ve %13,3'ünde birden fazla bakteri üremesi gözlendi. Her iki grupta izole edilen bakterilerin sayısında istatistiki fark bulunmazken, diyabetik olgularda gram-negatif bakteri kolonizasyonu daha yüksekti (sırasıyla ?20,129, p0,719 ve ?25,60, p0,018).Sonuç: Gram-negatif bakteriler diyabetik hastaların konjunktiva florasında daha sık olarak bulunmaktadır. Bu durum diyabetik hastalarda göz enfeksiyonlarını tedavi ederken dikkate alınmalıdır.
Objectives: To evaluate the conjunctival bacterial flora in diabetic patients and nondiabetic subjects.Materials and Methods: Fifty-three diabetic patients and 43 nondiabetic healthy individuals were included in the study. A specimen was taken from each participant for the study by rubbing a sterile cotton-tipped swab on the inferior palpebral conjunctiva of the right eye. Samples were incubated in blood agar, chocolate agar, eosin methylene-blue lactose sucrose agar and sabouraud 4% dextrose agar. Isolated microorganisms were identified using routine microbiological methods.Results: Rates for bacterial isolations were determined as 38.5% in diabetic patients and 34.9% in nondiabetic controls. Staphylococcus aureus was isolated in 30% of cases in the diabetic patient group, while 20% tested positive for Escherichia coli, 10% for coagulase-negative Staphylococcus, 10% for Klebsiella pneumoniae and 30% for multiple bacteria. In the non-diabetic group, 53.3% of patients were positive for Staphylococcus aureus while coagulase-negative Staphylococcus was isolated in 26.7%, Klebsiella pneumoniae in 6.7% and multiple bacteria in 13.3% of patients. Although there was no statistically significant difference in the number of isolated bacteria between the diabetic and nondiabetic groups, gram-negative bacterial colonization was significantly higher in diabetic patients (?20.129, p0.719 and ?25.60, p0.018, respectively).Conclusion: Gram-negative bacteria are more common in the conjunctival flora of diabetic patients. This should be considered by clinicians when treating ocular infections in diabetic patients.
Objectives: To evaluate the conjunctival bacterial flora in diabetic patients and nondiabetic subjects.Materials and Methods: Fifty-three diabetic patients and 43 nondiabetic healthy individuals were included in the study. A specimen was taken from each participant for the study by rubbing a sterile cotton-tipped swab on the inferior palpebral conjunctiva of the right eye. Samples were incubated in blood agar, chocolate agar, eosin methylene-blue lactose sucrose agar and sabouraud 4% dextrose agar. Isolated microorganisms were identified using routine microbiological methods.Results: Rates for bacterial isolations were determined as 38.5% in diabetic patients and 34.9% in nondiabetic controls. Staphylococcus aureus was isolated in 30% of cases in the diabetic patient group, while 20% tested positive for Escherichia coli, 10% for coagulase-negative Staphylococcus, 10% for Klebsiella pneumoniae and 30% for multiple bacteria. In the non-diabetic group, 53.3% of patients were positive for Staphylococcus aureus while coagulase-negative Staphylococcus was isolated in 26.7%, Klebsiella pneumoniae in 6.7% and multiple bacteria in 13.3% of patients. Although there was no statistically significant difference in the number of isolated bacteria between the diabetic and nondiabetic groups, gram-negative bacterial colonization was significantly higher in diabetic patients (?20.129, p0.719 and ?25.60, p0.018, respectively).Conclusion: Gram-negative bacteria are more common in the conjunctival flora of diabetic patients. This should be considered by clinicians when treating ocular infections in diabetic patients.
Açıklama
Anahtar Kelimeler
Konjunktival flora, Diyabetes mellitus, Gram-negatif bakteri, Conjunctival flora, Diabetes mellitus, Gram-negative bacteria
Kaynak
Türk Oftalmoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
45
Sayı
5
Künye
Adam, M., Balcı, M., Bayhan, H. A., İnkaya A. Ç., Uyar, M., Gürdal, C. (2015). Diyabetik ve Diyabetik Olmayan Bireylerde Konjunktiva Florası. Türk Oftalmoloji Dergisi, 45, 5, 193-196.
