İdiyopatik pulmoner fibrozisli hastalarda mikrorna, CCN2 ve TGF-β ekspresyon düzeylerinin değerlendirilmesi
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Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: MiRNA’lar biyolojik süreçlerde ve İPF gibi fibrotik hastalıklarda rol alan küçük
RNA molekülleridir. Son yıllarda yapılan çalışmalar; vücut sıvılarında tespit
edilebileceğini, hastalıkların tanı ve takiplerinde belirteç olarak kullanılabileceğini
göstermektedir. Bu projede amaç; İPF hastalarında fibrotik süreçten sorumlu olabilecek
CCN2 ve TGF-β genlerinin ve 21 miRNA’nın ekspresyon düzeylerinin periferik kanda
qPCR yöntemiyle değerlendirilmesidir.
Yöntem: Göğüs hastalıkları ana bilim dalı multidisipliner interstisyel akciğer hastalıkları
konseyinde değerlendirilmiş ve İPF tanısı almış 20 kişilik hasta grubu ile 20 sağlıklı
gönüllüden alınan periferik kan örneklerinde qPCR ile CCN2, TGF-β ve miRNA
ekspresyon analizleri yapıldı.
Bulgular: CCN2, TGF-β genlerinin ve miR’lerin ekspresyon düzeyleri belirlenerek hasta
ve kontrol grubu arasındaki fark istatistiksel yöntemler ile incelendi. TGF-β, miR29a/29b/29c-3p, miR-19a, miR-130a-3p, miR-140-5p, miR-320a, miR-155-5p, miR-21-5p,
miR-205-5p ekspresyonları hasta grubunda downregüle bulundu, miR-143-3p ekspresyonu
ise upregüle bulundu. CCN2, miR-145, miR-30a/30b/30d/30e-5p, miR-26a-5p, miR-32-5p,
miR-19b-1, miR-133 ve let-7d gruplar arasında ekspresyon farkı göstermedi. Hastalarda
miR-29a-3p ve miR-140-5p’nin sigara kullanımı ile; miR-155-5p, miR-140-5p ve miR320a’nın cinsiyete göre ekspresyon farkı sergilediği saptandı. miR-29a ve miR-143 ile
lojistik regresyon modeli oluşturuldu, ROC analizi ile miR-29a ve miR-143’ün hasta ve
kontrol grubunu ayırt etme gücü istatistiksel olarak yüksek bulundu.
Sonuç: Çalışmamızda, gen ve miRNA disregülasyonunun İPF hasta serumlarında RTqPCR gibi kolay ve rutin bir yöntemle tespit edilebileceği gösterilmiş; tanı, takip ve ilaç
yanıtı değerlendirmede kullanımının mümkün olabileceği tartışılmıştır. Literatüre katkı
sağlayacak yeni bulgular tespit edilmiş olup, bu bulgular ışığında yapılacak yeni
çalışmalara yön verecektir
Background/Aim: MiRNA’s are small RNA molecules that participate many biological processes and fibrotic diseases like IPF. Studies carried out in recent years; shows that it can be detected in body fluids and can be used as a marker in the diagnosis and follow-up of diseases. Aim in this project is evaluation of expression levels of CCN2 and TGF-β genes and 21 miRNA’s that can be responsible for the fibrotic process of IPF, using RTqPCR in peripheral blood samples. Methods: CCN2, TGF-β and miRNA expression levels were analyzed using qPCR in peripheral blood samples of 20 patients and 20 healthy volunteers. All of the patients were diagnosed of IPF in the multidiciplinary interstitial pulmonary diseases counsil of the chest diseases department. Result: Any alteration of the expression levels of CCN2, TGF-β genes and miRNA’s were statistically analyzed. It is revealed that; TGF-β, miR-29a/b/c-3p, miR-19a, miR-130a-3p, miR-140-5p, miR-320a, miR-155-5p, miR-21-5p, miR-205-5p expression levels were downregulated in the patients; in contrast to miR-143-3p expression, which was upregulated. CCN2, miR-145, miR-30a/b/d/e-5p, miR-26a-5p, miR-32-5p, miR-19b-1, miR-133 and let-7d showed no statistically significant difference between patient and control groups. It was demonstrated; miR-29a and miR-140 expressions were altered due to whether the patient was a smoker, while miR-155, miR-140 and miR-320a expressions showed gender differences. Logistical regression model with miR-29a and miR-143 expression profiles were created. It was demonstrated that; strength of differentiating case and control groups were statistically higher in miR-29a and miR-143, with ROC analysis. Conclusion: In this study; we demonstrated that, gene and miRNA dysregulations in blood samples of IPF patients could easily be detected by routinely used RT-qPCR method. The potentiality of them being used in diagnosis, follow-up and drug responsiveness was discussed. Our project will contribute to the literature with new findings and guide the other invetigators in this field.
Background/Aim: MiRNA’s are small RNA molecules that participate many biological processes and fibrotic diseases like IPF. Studies carried out in recent years; shows that it can be detected in body fluids and can be used as a marker in the diagnosis and follow-up of diseases. Aim in this project is evaluation of expression levels of CCN2 and TGF-β genes and 21 miRNA’s that can be responsible for the fibrotic process of IPF, using RTqPCR in peripheral blood samples. Methods: CCN2, TGF-β and miRNA expression levels were analyzed using qPCR in peripheral blood samples of 20 patients and 20 healthy volunteers. All of the patients were diagnosed of IPF in the multidiciplinary interstitial pulmonary diseases counsil of the chest diseases department. Result: Any alteration of the expression levels of CCN2, TGF-β genes and miRNA’s were statistically analyzed. It is revealed that; TGF-β, miR-29a/b/c-3p, miR-19a, miR-130a-3p, miR-140-5p, miR-320a, miR-155-5p, miR-21-5p, miR-205-5p expression levels were downregulated in the patients; in contrast to miR-143-3p expression, which was upregulated. CCN2, miR-145, miR-30a/b/d/e-5p, miR-26a-5p, miR-32-5p, miR-19b-1, miR-133 and let-7d showed no statistically significant difference between patient and control groups. It was demonstrated; miR-29a and miR-140 expressions were altered due to whether the patient was a smoker, while miR-155, miR-140 and miR-320a expressions showed gender differences. Logistical regression model with miR-29a and miR-143 expression profiles were created. It was demonstrated that; strength of differentiating case and control groups were statistically higher in miR-29a and miR-143, with ROC analysis. Conclusion: In this study; we demonstrated that, gene and miRNA dysregulations in blood samples of IPF patients could easily be detected by routinely used RT-qPCR method. The potentiality of them being used in diagnosis, follow-up and drug responsiveness was discussed. Our project will contribute to the literature with new findings and guide the other invetigators in this field.
Açıklama
Anahtar Kelimeler
İPF, MikroRNA, CCN2, TGF-β, RT-qPCR, Serum, IPF, MicroRNA, CCN2, TGF-β, RT-qPCR, Serum
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Metli, K. (2021). İdiyopatik pulmoner fibrozisli hastalarda mikrorna, CCN2 ve TGF-β ekspresyon düzeylerinin değerlendirilmesi. (Yayınlanmamış tıpta uzmanlık tezi) Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi Dahili Tıp Bilimleri Bölümü Tıbbi Genetik Anabilim Dalı, Konya.
