Konya İlinde Klinik Örneklerden İzole Edilen Mycobacterium tuberculosis Kompleks Suşlarının BirinciSeçenek Anti-tüberküloz İlaçlara Direnç Oranları
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Dosyalar
Tarih
2016
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada, 2014 ve 2015 yılını içeren iki yıllık süreçte, laboratuvarımızda izole edilen Mycobacterium tuberculosis kompleks izolatlarının birinci seçenek antitüberküloz ilaçlara duyarlılıklarının retrospektif olarak araştırılması amaçlanmıştır.Gereç ve Yöntem: Tüberküloz ön tanısı ile gönderilen şüpheli örnekler Ehrlich-Ziehl-Neelsen yöntemi ile boyanarak, mikroskobik olarak değerlendirilmiş, Löwenstein-Jensen besiyeri ve otomatize kültür sistemine (Mycolor TK, Salubris A.Ş., İstanbul) ekimleri yapılmıştır. Üreme görülen kültür tüplerinde Mycobacterium tuberculosis kompleks tanımlaması ve streptomisin, izoniazid, rifampisin, etambutol duyarlılıkları çalışılmıştır. Bulgular: Çalışmaya laboratuvara gönderilen 5406 klinik örnekten, Mycobacterium tuberculosis kompleks izole edilmiş 266 suş dâhil edilmiştir. Her hasta için tek bir suş çalışmaya alınmıştır. Streptomisin, izoniazid, rifampisin ve etambutole toplam direnç oranları sırasıyla %4.5, %6.4, %9 ve %4.5 olarak bulunmuştur. Hastaların %83.1'i tüm antibiyotiklere duyarlı olup, antimikobakteriyel ilaçlar içinde en yüksek direnç rifampisin için saptanmıştır. Çok ilaca direnç oranı ise %2.6 olarak bulunmuştur. Çalışmada ayrıca Ehrlich-ZiehlNeelsen boyama yöntemi ile otomatize kültür sistemi sonuçları karşılaştırmalı olarak değerlendirilmiştir. Sonuç: Tüberkülozda direnç paternlerinin izlenmesi, uygulanacak tedavi protokollerinin belirlenmesinde önemli olduğu gibi uzun vadede direnç gelişiminin önlenmesinde de yararlı olacaktır.
Objective: In this study we aimed to retrospectively analyse primary anti-tuberculosis drug susceptibilities of Mycobacterium tuberculosis complex strains, isolated from patients with initial diagnosis of tuberculosis during the two years period between 2014 and 2015 at our laboratory. Material and Methods: Clinical samples obtained from patients with suspected tuberculosis were stained with Ehrlich-Ziehl-Neelsen method and evaluated microscopically. After processing of samples, each sample was inoculated onto Löwenstein-Jensen medium and automated culture system (Mycolor TK, Salubris A.Ş., Istanbul) medium. If bacterial growth was detected, Mycobacterium tuberculosis complex typing was made and if requested antituberculosis drug susceptibilities for streptomycin, isoniazid, rifampicin and ethambutol were tested. Results: Out of 5406 clinical samples sent to the laboratory, a total of 266 Mycobacterium tuberculosis complex iolates were included in the study and only single sample from each patient was processed. Total resistance rates to streptomycin, isoniazid, rifampicin, and ethambutol were 4.5%, 6.4%, 9% ve 4.5%, respectively. Among antimycobacterial drugs, the highest resistance rate was found for rifampicin and 83.1% of the patients were susceptible to all of the agents tested. Multidrug resistance was determined as 2.6 percent. In the study, automated culture system was also evaluated in comparison with Ehrlich-Ziehl-Neelsen staining method. Conclusion: Monitoring of resistance patterns in tuberculosis is important in determining treatment protocols and will also be useful in preventing the development of resistance in long term.
Objective: In this study we aimed to retrospectively analyse primary anti-tuberculosis drug susceptibilities of Mycobacterium tuberculosis complex strains, isolated from patients with initial diagnosis of tuberculosis during the two years period between 2014 and 2015 at our laboratory. Material and Methods: Clinical samples obtained from patients with suspected tuberculosis were stained with Ehrlich-Ziehl-Neelsen method and evaluated microscopically. After processing of samples, each sample was inoculated onto Löwenstein-Jensen medium and automated culture system (Mycolor TK, Salubris A.Ş., Istanbul) medium. If bacterial growth was detected, Mycobacterium tuberculosis complex typing was made and if requested antituberculosis drug susceptibilities for streptomycin, isoniazid, rifampicin and ethambutol were tested. Results: Out of 5406 clinical samples sent to the laboratory, a total of 266 Mycobacterium tuberculosis complex iolates were included in the study and only single sample from each patient was processed. Total resistance rates to streptomycin, isoniazid, rifampicin, and ethambutol were 4.5%, 6.4%, 9% ve 4.5%, respectively. Among antimycobacterial drugs, the highest resistance rate was found for rifampicin and 83.1% of the patients were susceptible to all of the agents tested. Multidrug resistance was determined as 2.6 percent. In the study, automated culture system was also evaluated in comparison with Ehrlich-Ziehl-Neelsen staining method. Conclusion: Monitoring of resistance patterns in tuberculosis is important in determining treatment protocols and will also be useful in preventing the development of resistance in long term.
Açıklama
Anahtar Kelimeler
Mikrobiyoloji, Antitüberküloz Duyarlılığı, Direnç, Mycobacterium Tuberculosis, Antituberculosis Susceptibility, Resistance
Kaynak
Türk Mikrobiyoloji Cemiyeti Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
46
Sayı
4
Künye
Taşbent, F E., Doğan, M. (2016). Konya İlinde Klinik Örneklerden İzole Edilen Mycobacterium tuberculosis Kompleks Suşlarının Birinci Seçenek Anti-tüberküloz İlaçlara Direnç Oranları. Türk Mikrobiyoloji Cemiyeti Dergisi. 46, 4, 165-171.
