Türkiye'deki bazı kedi ırklarının mitokondrial DNA D-Loop polimorfizminin araştırılması
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Dosyalar
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada Van kedileri ile diğer bazı kedi ırklarındaki mitokondrial DNA (mtDNA) polimorfizmi ortaya koyulmuş, Van kedilerinde elde edilen mtDNA analiz sonuçları ile göz renkleri arasında filogenetik bir ilişkinin varlığı değerlendirilmiştir.Gereç ve Yöntem: Çalışılan 65 adet kan örneğinde bölgenin polimorfik olması, literatür bilginin yetersizliği ve farklı yöntemler kullanılması neticesinde örnek sonuçlarından sağlıklı bilgi alınamadığı için çalışmada 39 adet kedinin (25 Van kedisi, 3 İran kedisi, 3 Tekir kedisi, 7 Siyam kedisi ve 1 Ankara kedisi) analiz sonuçları sunuldu. Bazı yayınlardan aldığımız ve kendi dizayn ettiğimiz 15 primer denenmiş, 6 iyi çalışan primer ile çalışma tamamlanmıştır. Polimeraz Zincir Reaksiyonu ürünleri (PZR) CEQ-8000 Beckman Coulter Genetik Analiz Sistemi kullanılarak kapiller elektroforez ile ayrıştırılmış sekans dizilimleri belirlenmiştir.Bulgular: Van Kedilerinin her biri kendi grubu içerisinde tek gözlülük/çift gözlülük durumlarına göre gruplar arasında istatistiki fark (P0.022) tespit edildi. Elde edilen bu dizi analiz verilerine bakılarak Van kedilerinde %80.00 oranıyla tek gözlülük söylenebilir. MtDNA dizi analizinden seçilen 99 bç lik dar bir bölge değerlendirildi ve az sayıda örneğe rağmen elde edilen istatistiki fark değerlendirildi.
Yılmaz Sahin E, Altunok V, Kurar E. Investigation of genetic structure of various cat breeds by using D-Loop polimorphism in Turkey. Aim: In this study, mitochondrial DNA (mtDNA) polymorphism between the Van cats and the other cat breeds is revealed. It is evaluated whether there is any relationship between the result of the mtDNA analyses obtained from Van cats and eye colours.Materials and Methods: Although it is studied with 65 blood samples and different methods, polymorphic of the place and inadequate information of literature lead to unreliable sample results. For this reason, 39 analyses of cats (25 Van cats, 3 Iran cats 3 Tekir cats, 7 siyam cats and 1 Ankara cat) are presented. 15 primers which is designed by ourselves and gained by some publication are experimented. The study is completed with 6 good working primers. The products of Polymerase Chain Reaction (PCR) CEQ-8000 are determined by using the System of Genetic Analysis of Beckman Coulter and sequence of sequences resoluted with capillary electrophoresis are determined.Results: Each of every Van cats were evaluated in their groups according to one eye or double eye and statistical differentiation was found (P>0.022) between groups. By looking at the data of analysis, one eye at the rate of 80.00% can be said in Van cats. In this study, the selection of 99 bç, a narrow location obtained from mtDNA sequence analysis is evaluated and in spite of a small number of samples the acquired statistical difference is significant.Conclusion: We found significant differences in this study. This should be study with more samples and a large mtDNA D-Loop region.
Yılmaz Sahin E, Altunok V, Kurar E. Investigation of genetic structure of various cat breeds by using D-Loop polimorphism in Turkey. Aim: In this study, mitochondrial DNA (mtDNA) polymorphism between the Van cats and the other cat breeds is revealed. It is evaluated whether there is any relationship between the result of the mtDNA analyses obtained from Van cats and eye colours.Materials and Methods: Although it is studied with 65 blood samples and different methods, polymorphic of the place and inadequate information of literature lead to unreliable sample results. For this reason, 39 analyses of cats (25 Van cats, 3 Iran cats 3 Tekir cats, 7 siyam cats and 1 Ankara cat) are presented. 15 primers which is designed by ourselves and gained by some publication are experimented. The study is completed with 6 good working primers. The products of Polymerase Chain Reaction (PCR) CEQ-8000 are determined by using the System of Genetic Analysis of Beckman Coulter and sequence of sequences resoluted with capillary electrophoresis are determined.Results: Each of every Van cats were evaluated in their groups according to one eye or double eye and statistical differentiation was found (P>0.022) between groups. By looking at the data of analysis, one eye at the rate of 80.00% can be said in Van cats. In this study, the selection of 99 bç, a narrow location obtained from mtDNA sequence analysis is evaluated and in spite of a small number of samples the acquired statistical difference is significant.Conclusion: We found significant differences in this study. This should be study with more samples and a large mtDNA D-Loop region.
Açıklama
Anahtar Kelimeler
Veterinerlik, mtDNA, Sequence Analysis, Cat, Van Cat, Dizi Analizi, Kedi, Van Kedisi
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
32
Sayı
4
Künye
Yılmaz Şahin, E., Altunok, V., Kurar, E. (2016). Türkiye'deki bazı kedi ırklarının mitokondrial DNA D-Loop polimorfizminin araştırılması. Eurasian Journal of Veterinary Sciences, 32, 4, 229 - 235.
