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Öğe Assessment of Data Regarding Thalassemias and Hemoglobin Variants From a Tertiary Referral Hospital Laboratory in Turkey(2017) Özer, Nejla; Uysal, Saliha; Güngören, Merve Sibel; Aköz, Mehmet; Balcı, TevfikAmaç: Hemoglobinopatiler, globin fonksiyonlarını bozan bir grup hastalıktır. HPLC, hemoglobinopati taramalarında tüm dünyada en sık kullanılan tekniktir. Çalışmada, Türkiye'de bir üçüncü basamak hastane laboratuvarında 3 yıllık retrospektif hemoglobinopati sıklığı değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Üniversite hastanemize başvuran 2461 hastanın 3 yıllık laboratuvar ve hastane kayıtları incelenmiştir. Hemoglobinopati taramasında Tosoh G8 HPLC cihazı kullanılmıştır Bulgular: HbA2%3,5 olan 668, %3,5HbA2%4 olan 19 sonuç saptanmıştır.19 sonuçtan 10'u betatalasemi minör olarak kabul edilmiştir. HbA2 %4 olan 649 sonuç vardır ve sonculara göre 602 hasta beta-talassemi minör olarak kabul edilmiştir. HbA2 düzeylerine göre, hastaların %25'i beta-talasemi minör olarak değerlendirilmiştir. HbF%2 olan 391 hasta saptanmış ve HbA2%3,5 olan 138 kayıt ayrıntılı incelemeye alınmıştır. Varyant analizi veri kümesi, 53 hasta içermektedir. HbA2%3,5 ve HbF%2 olan, varyant pikleri mevcut kayıtlar incelenmiş ve 28 vaka saptanmıştır. En sık görülen Hb varyantları sırasıyla HbH, HbS, HbD, HbC, HbE ve HbO-Arab'dır. Sonuç: Çalışmada en sık varyantlar sırasıyla HbH, HbAS, HbAD, HbAC, HbAE ve HbO-Arab olarak bulunmuştur. Beta talasemi minör sıklığı ülkemizin gelen popülasyonundan yüksek olarak %25 bulunmuştur. Özellikle hematolojik malignensiler, herediter sferositoz ve demir eksikliği anemisi gibi eşlik eden durumlar hemoglobinopati değerlendirmesinde akılda tutulmalıdır.Öğe Can YKL-40 be an Inflammatory Biomarker in Vitamin D Deficiency?(Verlag Hans Huber, 2019) Can, Ummugulsum; Uysal, Saliha; Ugur, Ayse Ruveyda; Toker, Aysun; Aslan, Uysaler; Hidayetoglu, Bahauddin TahaVitamin D deficiency is associated with several non-homeostatic conditions and/or diseases like inflammation, atherosclerosis, cardiovascular disease and mortality. YKL-40 is a glycoprotein, secreted by macrophages, neutrophils and different cell types and it is also associated with inflammation and pathological tissue remodeling. In this study, we aimed to evaluate relationship between the proinflammatory biomarkers YKL-40 and hs-CRP levels and vitamin D deficiency. Our study group includes 45 subjects with vitamin D deficiency (Group 1) (20 M, 25 F; mean age 37.72 +/- 7.70 years) and 40 age and sex-matched healthy subjects with normal serum levels of vitamin D (Group 2) (19 M, 21 F; mean age 39.26 +/- 7.41 years). Plasma 25 (OH) vitamin D levels were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. Plasma YKL-40 analysis was performed by ELISA. Serum hs-CRP levels were measured by nephelometric method. Plasma vitamin D levels below 20 ng/mL were accepted as vitamin D deficiency. Although we could not find any significant differences by means of serum hs-CRP levels between Group 1 and Group 2 (2.21 (0.27-11.70); 1.79 (0.16-9.85) mg/L, p = 0.247), plasma YKL-40 levels were significantly higher in group 1 than group2 (70.47 (17.84-198.50); 47.14 (4.80-135.48) ng/mL, p = 0.047). In literature, vitamin D deficiency is associated with inflammation. In our study, we found similar hs-CRP levels between groups and higher YKL-40 levels in group 1. Vitamin D deficiency may be related to high YKL-40 levels in terms of causing chronic inflammation.Öğe Exploratory study to evaluate plasma vasopressin and apelin-13 levels in children with attention-deficit hyperactivity disorder(Wiley, 2016) Bilgic, Ayhan; Toker, Aysun; Uysal, SalihaAimVasopressin exerts robust influences on social communication and behavior in humans. Apelin is a relatively novel neuropeptide that could counteract vasopressin's actions and has been shown to be closely related with a broad range of physiological functions. Abnormalities in vasopressin and apelin have been detected in a variety of psychiatric disorders, but their relation to attention-deficit hyperactivity disorder (ADHD) is unknown. In the present study, we explored the plasma levels of vasopressin and apelin-13 in children with ADHD. MethodsThirty-four children with ADHD and 36 healthy controls were enrolled in this study. The severity of ADHD symptoms was assessed via Conners' Parent Rating Scale and Conners' Teacher Rating Scale. Plasma levels of vasopressin and apelin-13 were measured using commercial enzyme-linked immunosorbent assay kits. ResultsThe mean plasma apelin-13 levels were significantly higher in male children with ADHD than in male control subjects; no significant difference was found between the groups for plasma apelin-13 levels in girls or in the entire subject cohort. Plasma vasopressin levels did not show any significant differences between groups. There were no significant correlations between plasma levels of these neuropeptides and scores for Conners' Parent Rating Scale and Conners' Teacher Rating Scale. ConclusionOur results suggest a sex-specific association between plasma apelin-13 levels and ADHD. Apelin-13 may play a role in the etiopathogenesis of ADHD either with a direct impact on the apelin receptor or via its opposing effect on the vasopressinergic system.Öğe Ischemia modified albumin Changes During OGTT in Pregnant Women with Gestational Diabetes Mellitus(Canadian Soc Clinical Investigation, 2015) Uysal, Saliha; Toker, Aysun; Akcay, Merve; Gokce, Hacer R.Purpose: Published results regarding the oxidative status in gestational diabetes mellitus (GDM) are conflicting. As a biomarker for acute ischemia, ischemia modified albumin (IMA) is associated with pregnancy, especially complicated pregnancies. Our aim was to investigate serum IMA, malondialdehyde (MDA) and total antioxidant status (TAS) levels in patients with GDM. Changes in serum IMA levels after glucose loading during oral glucose tolerance test (OGTT) in pregnant women were also analysed. Methods: OGTT were performed on age-matched participants. Pregnant with abnormal glucose challenge test (GCT), normal glucose tolerance (NGT) (Group 1, n=32) and GDM (Group 2, n=28), defined by ADA criteria, were also performed. Serum IMA, MDA and TAS levels were determined by colorimetric methods. Results: Serum MDA levels in group 2 were significantly higher compared with group 1 (p<0.05) There were no significant differences in serum TAS and IMA levels between two groups (p>0.05). Similar serum IMA levels were obtained in both groups, both at baseline and in the following serum samples during OGTT. In GDM group, there were significant correlations between MDA levels with baseline IMA levels (r=0.51, p=0.005) and first hour IMA levels (r=0.39, p=0.039). Conclusions: Increased oxidative stress may play important role in GDM pathogenesis. Glucose loading does not appear to affect IMA formation; however, larger studies are necessary to understand a wider range of biochemical parameters in large population-based studies of patients with GDM.Öğe Ischemia-Modified Albumin and Atherosclerosis in Patients With Familial Mediterranean Fever(Sage Publications Inc, 2016) Kucuk, Adem; Uslu, Ali Ugur; Arslan, Sevket; Balta, Sevket; Ozturk, Cengiz; Uysal, Saliha; Yilmaz, RamazanThe constriction of vessels due to atherosclerotic lesions causes hypoxia/ischemia and oxidative changes resulting in transformation of free albumin to ischemia-modified albumin (IMA) in the circulation and increased carotid intima-media thickness (cIMT). We investigated the reliability of IMA increase in evaluating atherosclerosis in patients with familial Mediterranean fever (FMF) compared with cIMT. Patients with FMF (n = 58) diagnosed by the Tel-Hashomer criteria in attack-free period and 38 healthy people were included in the study. Patient demographics as well as the clinical and laboratory characteristics of the healthy controls and patients with FMF were noted. The IMA levels and cIMT in patients with FMF were 0.30 +/- 0.09 absorbance units (ABSUs) and 1.12 +/- 0.27 mm, respectively, and in the control group, IMA levels and cIMT were 0.25 +/- 0.07 ABSU and 0.74 +/- 0.26 mm, respectively. The IMA levels and cIMT were significantly higher in patients with FMF than in controls (P = .020 and P < .0001, respectively). The IMA values showed positive correlation with cIMT in patients with FMF(r = .302, P = .041). Our results reveal that IMAan oxidative stress markermay be an indicator of atherosclerosis in patients with FMF. This finding deserves further investigation.Öğe Kronik böbrek ve renal transplant hastalarında decoy reseptör 3´ ün inflamasyon ve aterosklerozdaki rolü(Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi, 2016) Uysal, Saliha; Toker, AysunKronik Böbrek Hastalığında, kronik inflamasyon ve aterosklerozla ilişkili olarak kardiyovasküler hastalık riski artmaktadır. Hemodiyaliz ve periton diyaliz alan hastalara göre belirgin bir düzelme görülse de renal transplantasyon sonrası inflamatuvar süreç devam etmektedir. TNF reseptör ailesinin bir üyesi olan Decoy reseptör 3 (DcR3), inflamasyon ve ateroskleroz ile ilişkili bulunmuştur. Bu çalışmadaki amacımız, kronik böbrek ve renal transplant hastalarında; DcR3 seviyeleri ile inflamasyon ve ateroskleroz arasındaki ilişkiyi göstermektir. Bu amaçla, serum DcR3, hücreler arası adezyon molekülü-1 (intercellular adhesion molecule-1 (ICAM-1)), vasküler hücre adezyon molekülü-1 (vascular cell adhesion molecule-1 [VCAM-1]), interlökin-8 (interleukin-8 [IL-8]) seviyelerinin ölçülmesi, endotelyal disfonksiyonun karotid intima-media kalınlığı (KİMK) ve karotid arter plak varlığı ile değerlendirilmesi hedeflenmiştir. Gereç ve Yöntem: Çalışmaya 50 renal transplantasyon yapılan (Grup 1), 40 hemodiyaliz (HD) alan (Grup 2), 30 pre-diyaliz KBY (Grup 3) ve 30 sağlıklı kontrol (Grup 4) olmak üzere toplam 150 birey dahil edildi. Serum DcR3, VCAM-1, ICAM-1 ve IL-8 seviyeleri ELISA yöntemiyle çalışıldı. KİMK ve karotid arter plak varlığı non-invaziv olarak ultrason probuyla gerçekleştirildi. Bulgular: Serumda çalışılan tüm parametreler ve KİMK, HD ve pre-diyaliz KBY gruplarında renal transplantasyon ve kontrol gruplarına kıyasla yüksek bulundu. Renal transplantasyon grubunun sonuçları ise kontrol grubundan yüksekti. HD ve pre-diyaliz KBY grupları arasında fark bulunmadı. Hasta gruplarının plak varlıkları arasında fark yoktu. Kontrol grubunda plağa rastlanmadı. Pre-diyaliz KBY grubu kendi içinde değerlendirildiğinde KİMK dışında hiçbir parametrede evreler arasında fark saptanmadı. En yüksek KİMK değerlerinin evre 3'te olduğu görüldü. Sonuç: Renal transplantasyon, inflamatuvar süreçte belirgin bir düzelme sağlasa da inflamasyon sürecini tamamen geri döndürememektedir. Pre-diyaliz KBY ve HD grubunda üremik ortamla ilişkilendirilebilecek inflamatuvar sürecin ateroskleroza zemin hazırladığı söylenebilir. Pre-diyaliz KBY ve HD gruplarında artmış KİMK ve yüzde plak varlıkları bu durumu desteklemektedir. Anahtar kelimeler: DcR3, inflamasyon, ateroskleroz, KİMK, renal transplantasyonÖğe PLASMA ARGININE VASOPRESSIN AND APELIN LEVELS IN CHILDREN WITH ATTENTION-DEFICIT/HYPERACTIVITY DISORDER(Elsevier Science Inc, 2016) Bilgi, Ayhan; Toker, Aysun; Uysal, Saliha[Abstract Not Availabe]Öğe The role of decoy receptor 3 in inflammation and atherosclerosis in patients with chronic kidney disease and renal transplant patients(Soc Espanola Nefrologia Dr Rafael Matesanz, 2023) Uysal, Saliha; Toker, Aysun; Turkmen, Kultigin; Keskin, SuatIntroduction: The cardiovascular risk has been increased in chronic kidney disease associated with chronic inflammation and atherosclerosis. Decoy receptor 3, is a member of the TNF receptor superfamily and associated with inflammation and atherosclerosis. The aim of our study is to determine the relationship, between serum DcR3 levels and inflammatory markers in patients with renal transplantation, those receiving dialysis treatment and cases with chronic renal failure that did not receive replacement therapy, and to evaluate their correlation with USG findings. Material and methods: A total of 150 patients aged between 22-86 years, consisting of 4 groups, namely renal transplantation, dialysis, predialysis chronic kidney disease and control groups, were included in the study. Serum decoy receptor 3, VCAM-1, ICAM-1 and IL-8 measured with ELISA method. Carotid intima-media thickness and presence of carotis arter plaque performed by ultrasound probe, non-invasively. Results: All serum markers were higher in dialysis and pre-dialysis chronic kidney disease groups compared to renal transplant and control groups (p < 0.05). Serum decoy receptor 3 level (median(min-max)) of renal transplant group (0.49 ng/mL (0.19-1.65)) was higher than control group (0.35 ng/mL (0.19-2.22)). There was no difference between patients receiving dialysis (0.89 ng/mL (0.41-4.98)) and patients with pre-dialysis chronic kidney disease (0.71 ng/mL (0.29-1.68)). There was no difference between patient groups in terms of the presence of plaque. Conclusion: Although renal transplantation provides a significant improvement in the inflammatory process, not return completely. Inflammatory process associated with uremic milieu may predispose to atherosclerosis in patients with pre-dialysis chronic kidney disease and hemodialysis patients. (c) 2022 Sociedad Espanola de Nefrologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Öğe Serum ischemic modified albumin (IMA) concentration and IMA/albumin ratio in patients with hepatitis B-related chronic liver diseases(2017) Yavuz, Fatma; Bıyık, Murat; Asıl, Mehmet; Dertli, Ramazan; Demir, Ali; Polat, Hakkı; Uysal, Saliha; Ataseven, HüseyinBackground/aim: Albumin is the most important protein synthesized by the liver. Posttranscriptional changes occur in the molecular structure of albumin due to various factors and isoforms arise. Ischemic modified albumin (IMA) is one such isoform. This study was conducted to evaluate serum IMA concentrations in patients with hepatitis B virus (HBV)-related chronic liver diseases. Materials and methods: This study included 74 treatment-naive chronic hepatitis B patients, 25 patients with HBV-related cirrhosis, and 49 healthy controls. Serum IMA concentration was measured spectrophotometrically using the albumin cobalt binding test. Results: The mean IMA concentrations in the chronic hepatitis B group and healthy controls were 0.33 ± 0.11 ABSU and 0.27 ± 0.70 ABSU, respectively, and the difference was statistically significant. Mean IMA/albumin ratios (IMAR) in the chronic hepatitis B and control groups were 0.08 ± 0.04 and 0.06 ± 0.17, respectively, and the difference was also statistically significant (P > 0.001). Higher serum IMA concentrations and IMAR were detected in patients with advanced fibrosis. Conclusion: Serum IMA concentration and IMAR are increased in patients with HBV-related chronic liver diseases and IMA and IMAR are associated with the degree of liver fibrosis. IMA and IMAR may have potential use as noninvasive markers of fibrosis in chronic hepatitis B patients.Öğe Serum soluble TWEAK levels in non-alcoholic fatty liver disease(2019) Dertli, Ramazan; Yolaçan, Ramazan; Keskin, Muharrem; Kayar, Yusuf; Ataseven, Hüseyin; Polat, Hakkı; Ünver, Nurcan; Demir, Ali; Asıl, Mehmet; Bıyık, Murat; Uysal, SalihaAim: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. The exact pathogenesis of NAFLD has not been fully elucidated. Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a member of TNF superfamily and it hasbeen implicated in the pathogenesis of several diseases including liver inflammation and fibrosis. Current study was conducted to evaluate serum sTWEAK levels in patients with NAFLD. Material and Methods: Seventeen patients with biopsy proven non-alcoholic steatohepatitis (NASH), 22 patients with simple hepatosteatosis and 30 healthy controls were included in the study and serum sTWEAK concentrations were measured using commercial ELISA kits. Results: Mean serum sTWEAK concentration was significantly lower in the NASH group when compared to the simple hepatosteatosis group and healthy controls (199.6101.2 pg/mL, 246.165.7 pg/mL and 277.6117.6 pg/mL respectively, p0.029). ROC analysesfor sTWEAK to differentiate NASH patients from healthy controls and from simple hepatosteatosis revealed that AUC for sTWEAK was 0.712 (%95 CI, 0.543-0.880). For the specified cut off value, 171.1 pg/mL positive and negative predictive values calculated were 64.3% and 85.5% respectively. Conclusion: Serum sTWEAK concentration is decreased in patients with NASH when compared to patients with simple hepatosteatosis and healthy controls.